怎样学习英语好一点

发誓牛津/柯林斯词典，尽在百度翻译APP详细释义双语例句词语用例英英释义词典释义讲解0:59v.咒骂;诅咒;说脏话;郑重承诺;发誓要;表示决心要;赌咒发誓地说;肯定地说高考 / CET4 / 考研 / IELTS / TEM4变形动词第三人称单数:swears 现在分词:swearing 过去分词:sworn 过去式:swore 双语例句全部发誓咒骂宣誓1.It"s wrong to swear and shout破口大骂是不对的。《柯林斯高阶英汉双解学习词典》2.Alan swore that he would do everything in his power to help us艾伦许诺要尽其所能帮助我们。《柯林斯高阶英汉双解学习词典》3.I swear I"ve told you all I know我发誓我已经把我所知道的全都告诉你了。《柯林斯高阶英汉双解学习词典》查看更多例句词语用例词语搭配短语动词习语swear与以下词性连用n.1.swear words说脏话2.swear allegiance宣誓效忠3.swear an oath发誓adv.1.solemnly swear郑重保证

swear有脏话的意思。swear的意思指咒骂，说脏话，诅咒发誓；保证；诅咒；咒骂；宣誓；推荐；宣誓就职；宣誓保证；使宣誓；命…宣誓。第三人称单数是swears。现在分词是swearing。过去式是swore。过去分词是sworn。短语搭配：swear word诅咒语；咒骂语。swear like a trooper破口大骂。swear something out经宣誓保证所控属实后发给。swear to发誓。swear by以…的名义起誓。swear at咒骂；骂；与…冲突。swear的例句如下：1、I swear there"s a female conspiracy against men！He complained, tongue-in-cheek.我敢保证有一个女人反对男人的阴谋！他故作正经地说。2、He forced them to swear an oath of loyalty to him.他强迫他们宣誓要对他效忠。3、I asked him if he would swear a statement to this effect.我问他是否愿意发誓作出大意如此的声明。4、I couldn"t swear to it, but I"m pretty sure it"s his writing.我不能保证，但我相当肯定这是他的笔迹。5、Don"t swear. / Don"t use dirty language.说话别带脏字。6、I swear by all I hold dear that I had nothing to do with it.我以我所有最珍贵的东西起誓我和这件事情毫无关联。7、It"s not right to swear at people.可不作兴骂人。

swearv.发誓; 咒骂n.发誓，宣誓; 咒骂过去式： swore过去分词： sworn现在分词： swearing第三人称单数： swears

可译为，“诅咒，发誓”。

发誓;宣誓TheyrefusedtoswearonaBible.他们拒绝手按《圣经》发誓。2.诅咒;骂脏话[(+at)]Whatisthewomanswearingat?那女人在咒骂什么?及物动词vt.1.发(誓);宣(誓)Beforegivingevidenceyouhavetoswearanoath.在作证之前你得先发誓。2.发誓要;起誓保证[+to-v][+(that)]Thewitnesssworetotellthetruth.证人起誓保证讲真话。3.【口】断言;保证说[W][Y][+(that)]Hesworethathehadnevertalkedwiththegirl.他发誓说,他从未与那个姑娘说过话。4.使宣誓[(+to)]Youmustswearhimtosilence.你一定要他发誓保持沉默。5.使宣誓就任[H][(+in)]ThenewpresidentwillbesworninJanuary.新总统将在一月份宣誓就职。6.宣誓对(罪行)作证Hesworetreasonagainsthisfriend.他宣誓对他的朋友犯有叛国罪作证。

宣誓换言之，也称为诅咒，总之，是fownded在每一个语言和文化在世界上。 虽然它们的起源仍然有点神秘的，大部分研究人员相信，宣誓词演变出一anciene议案发言的话有神奇的力量。 如今，很少人相信，在这个概念，但人的反应（相关听证会或书面宣誓字）仍是强大的。 宣誓就职，往往是本能的情绪反应，以意想不到的或痛苦的事件。 当人们宣誓，思想和感觉，通过他们的大脑几乎深深沉迷。因此，站在该neroaction *宣誓就职。 科学家们正在获得深入了解不同regious脑沟通。 宣誓就职，有其他的职能，以及除了本能的情绪反应。 举例来说，在证据的情况下宣誓就职，其实是一种方式*列表示，它有帮助的作用，防止身体暴力。 尽管宣誓就职，可能有其用途，它始终是进攻性的人。 因此，在大多数情况，这是很重要的发现更多的respectical的方式来表达自己。

okk many choose maybe you more like slang？

vow 和 swear 有同样的意思但是vow 绝对不常用swear 经常用来说人骂人的比如在我学校里老师经常说stop swearing!or no swearing words!就是不要骂人的意思

You can tell your old man you"ll do some largemouth fishing another time你可以和你老爸说下次再去钓大鱼You just got too much on your plate to bait and cast a line因为你要做的事情太多了，所以你没有办法收鱼线You can always put a rain check in his hand till you can"t你可以一直和你老爸说等一会儿，直到你不能You can keep putting off forever with that girl who"s heart you hold你可以永远和你深爱的女孩子在一起Swearing that you"ll ask some day further down the road保证你会在之后的某一天会想起的You can always put a diamond on her hand till you can"t你可以一直把钻石戴在那个女孩手上If you got a chance, take it, take it while you got a chance如果你有机会，抓住它，一定要抓住它If you got a dream, chase it, "cause a dream won"t chase you back如果你有梦想，那就去追逐它，因为梦想不会追逐你If you"re gonna love somebody, hold "em as long and as strong and as close as you can如果你爱着一个人，就尽自己可能长时间的紧紧抱住她/他Till you can"t直到你不能There"s a box of greasy parts sitting in the trunk of that "65那辆65年汽车的后备箱还有一盒油腻腻的零件Still waiting on you and your granddad to bring it back to life还在等着你和爷爷去把他们复活使用呢e16061f06577599a3c01c1f40a20f31a意公东irfc4

一点都不好，不是我说的

does do did why

《疯狂外星人》主题曲：Feel The Night

誓师大会 institution; [例句]参加北京远南运动会的日本代表团举行誓师大会The Japanese delegation participating in the Beijing Far South Sports Meet holds swearing in ceremony

fergie的《Labelsorlove》shoppingforlabels,shoppingforlovemanoloandlouis,it"salli"mthinkingofshoppingforlabels,shoppingforlovemanoloandlouis,it"salli"mthinkingofialreadyknowwhatmyaddictionisibelookingforlabels,iain"tlookingforloveishopforpurseswhileiwalkoutthedoordon"tcry,buyabagandthengetoveritand,i"mnotconcernedwithallthepoliticsit"salotofmeniknowicouldfindanotherone.whatiknowisthati"malwayshappywheniwalkoutthestore,storei"mguessingsupercalifragi-sexy,nothingtobeplayingwithilovehim,hatehim,kisshim,disshim,trynatowalkamileinmykickswww.feiyes.netlove"slikearunwaybutwhichonedoilovemorenoemotionalbaggage,justbigbagsfilledwithdiorlove"slikearunway,sowhat"sallthefussingforlet"sstopchasingthemboysandshopsomemoreiknowimightcomeoffasnegativeibelookingforlabels,iain"tlookingforloverelationshipsareoftensohardtotameapradadresshasneverbrokenmyheartbeforeand,ballin"ssomethingthati"mfedupwithi"mmadothedamnthing,watchmedothedamnthingcauseiknowthatmycreditcardwillhelpmeputouttheflamesi"mguessingsupercalifragi-sexy,nothingtobeplayingwithilovehim,hatehim,kisshim,disshim,trynatowalkamileinmykickslove"slikearunwaybutwhichonedoilovemorenoemotionalbaggage,justbigbagsfilledwithdiorlove"slikearunway,sowhat"sallthefussingforlet"sstopchasingthemboysandshopsomemoregucci,fendi,pradapurses,purchasingthemfinerthingsmentheycomeadimeadozen,justgivemethemdiamondringsi"mintoalotofbling,cadallic,chanelandcoachfellasboastbuttheycan"treallyhandlemyfemaleapproachbuyingthingsishardtosayrockingchristianaudigier,manolo,polo,takingphotosinmycartiersowecan"tgoalltheway,iknowyoumighthateitbuti"mashopforlabelswhilethemladieslayandwaitforlovelove"slikearunwaybutwhichonedoilovemorenoemotionalbaggage,justbigbagsfilledwithdiorlove"slikearunway,sowhat"sallthefussingforlet"sstopchasingthemboysandshopsomemoreshoppingforlabels,shoppingforlovemanoloandlouis,it"salli"mthinkingofshoppingforlabels,shoppingforlovemanoloandlouis,it"salli"mthinkingof

swearKK: []DJ: []vi.1. 发誓;宣誓They refused to swear on a Bible.他们拒绝手按《圣经》发誓。2. 诅咒;骂脏话[(+at)]What is the woman swearing at?那女人在咒骂什么?vt.1. 发(誓);宣(誓)Before giving evidence you have to swear an oath.在作证之前你得先发誓。2. 发誓要;起誓保证[+to-v][+(that)]The witness swore to tell the truth.证人起誓保证讲真话。3. 【口】断言;保证说[W][Y][+(that)]He swore that he had never talked with the girl.他发誓说,他从未与那个姑娘说过话。4. 使宣誓[(+to)]You must swear him to silence.你一定要他发誓保持沉默。5. 使宣誓就任[H][(+in)]The new president will be sworn in January.新总统将在一月份宣誓就职。6. 宣誓对(罪行)作证He swore treason against his friend.他宣誓对他的朋友犯有叛国罪作证

在英语合成词中，合成名词（compound nouns）数量很多，而且可以按照既定规律自行构造新词。 　　合成名词构造法主要有下列八种： 　　①名词+名词，如 　　daybreak, sunrise, cattleshed, house-keeper, shoe-maker, headache, night-club, pocket-knife, arm-chair, wine-glass, ink-stand, man-servant, maid-servant, steamboat, goldsmith, newspaper, lawsuit等。 　　②形容词+名词，如 　　blackboard, commonweath, highway, stronghold, sweetheart, easy-chair, grandson, blueprint, deadline, high-brow, lazy-bones等。 　　③动词+名词，如 　　drawbridge, grindstone, playground, pickpocket, breakfast, cut-throat, makeshift, sing-song, turn-coat, washbasin 等。 　　④副词虚词+名词，如 　　afterthought, offshoot, outbreak, byway, bylaw, outpost, overcoat, underclothes, outgrowth, downpour, upkeep, bystander等。 　　⑤副词虚词+动词，如 　　income, outlet, outlook, onset, outfit, upstart, inlet, offspring, outburst, offset等。 　　⑥动词+副词，如 　　die-hard, breakdown, drawback, set-up, break-up, farewell, standstill, feedback等。 　　⑦名词+动名词，如 　　book-keeping, town-planning, letter-writing, word-building, tiger-hunting, paper-manufacturing, bill-collecting, car-repairing, television-advertising, window-shopping, day-dreaming, photocopying, handwriting等。 　　⑧其他，如 　　man-of-war, forget-me-not, son-in-law, mother-in-law, commander-in-chief, editor-in-chief, merry-go-round, hide-and-seek, stay-at-home, jack-in-the-box等。 　　在这8种构词法中，第7类“名词+动名词”最富生命力。此外，动名词还可以下列三形式构成合成名词： 　　一动名词+名词，如 　　drinking water, listening material, fishingrod, waiting-room, looking-glass, stepping-stone, running mate 等。 　　二动名词+副词虚词，如 　　swearing-in, going-over 　　三副词+动名词，如 　　uprising, well-being 等。

更新1: 真系 急!急!急! 你的问题: 动词只言及加 ing 转名词 不是转形 加多或减去字母 例如 write 要减去e 才加 ing writing swim 要加多个 m swimming 不知道是否只准加 ing ? 下列动词只是加 ing. 列出的名词是正式名词 字典可查到 可数名词 (countable noun) 可加 s 例如finding (singular) – findings (plural) 1. build — building (countable noun) 2. carve — carving countable noun) 3. draw — drawing (countable noun) 4. dwell — dwelling (countable noun) 5. feel — feeling (countable noun) 6. find — finding (countable noun) 7. hold —holding (countable noun) 8. kill — killing (countable noun) 9. meet – meeting (countable noun) 10. open — opening (countable noun) 11. read — reading (countable noun / uncountable noun) 12. shoot — shooting (countable noun) 13. spell — spelling (countable noun / uncountable noun) 14. teach — teaching (countable noun / uncountable noun) 15. warn — warning (countable noun) According to what you said verb to noun(+ing) meaning gerund. A gerund v to n(grammar) me a noun in the form of the present participle of a verb(ending in -ing)eg:-travelling in eg:-I preferred travelling to reading. All other eg.should be done by yourself. (1)read---reading (2)speak--speaking (3)spell--spelling (4)stand--standing (5)swear--swearing (6)swim--swimming (7)take--taking (8)teach--teaching (9)tell--telling (10)think--thinking (11)typeset--typesetting (12)understand--understanding (13)win--winning (14)write--writing (15)listen--listening

注意你的言辞英文为：watch you mouth。Watch your mouth！是一句很实用的英语口语表达。这个口语表达的意思是 Pay attention to what you are saying! Do not say anything rude! 就是“注意言辞，不要说脏话，嘴巴放干净点儿”。具体可见以下例句：1、Hey, keep your shirt on.Watch your mouth! This is not the right occasion.嗨，耐心点儿，注意你的言辞。这不是合适的场合。2、Hey, watch your mouth. No swearing.嘿，嘴巴放干净点儿，别说脏话。3、Watch your mouth! How would you feel if someone said that about you?注意你的言辞！如果别人这样说你，你会怎么想？另外，这个口语表达还可以说成：Watch your tongue! Watch your language! 意思是一样的。

You can tell your old man you"ll do some largemouth fishing another time你可以和你老爸说下次再去钓大鱼You just got too much on your plate to bait and cast a line因为你要做的事情太多了，所以你没有办法收鱼线You can always put a rain check in his hand till you can"t你可以一直和你老爸说等一会儿，直到你不能You can keep putting off forever with that girl who"s heart you hold你可以永远和你深爱的女孩子在一起Swearing that you"ll ask some day further down the road保证你会在之后的某一天会想起的You can always put a diamond on her hand till you can"t你可以一直把钻石戴在那个女孩手上If you got a chance, take it, take it while you got a chance如果你有机会，抓住它，一定要抓住它If you got a dream, chase it, "cause a dream won"t chase you back如果你有梦想，那就去追逐它，因为梦想不会追逐你If you"re gonna love somebody, hold "em as long and as strong and as close as you can如果你爱着一个人，就尽自己可能长时间的紧紧抱住她/他Till you can"t直到你不能There"s a box of greasy parts sitting in the trunk of that "65那辆65年汽车的后备箱还有一盒油腻腻的零件Still waiting on you and your granddad to bring it back to life还在等着你和爷爷去把他们复活使用呢3b6c77ec1b27b76ce73e154580e8332a志流新认样b059

You can tell your old man you"ll do some largemouth fishing another time你可以和你老爸说下次再去钓大鱼You just got too much on your plate to bait and cast a line因为你要做的事情太多了，所以你没有办法收鱼线You can always put a rain check in his hand till you can"t你可以一直和你老爸说等一会儿，直到你不能You can keep putting off forever with that girl who"s heart you hold你可以永远和你深爱的女孩子在一起Swearing that you"ll ask some day further down the road保证你会在之后的某一天会想起的You can always put a diamond on her hand till you can"t你可以一直把钻石戴在那个女孩手上If you got a chance, take it, take it while you got a chance如果你有机会，抓住它，一定要抓住它If you got a dream, chase it, "cause a dream won"t chase you back如果你有梦想，那就去追逐它，因为梦想不会追逐你If you"re gonna love somebody, hold "em as long and as strong and as close as you can如果你爱着一个人，就尽自己可能长时间的紧紧抱住她/他Till you can"t直到你不能There"s a box of greasy parts sitting in the trunk of that "65那辆65年汽车的后备箱还有一盒油腻腻的零件Still waiting on you and your granddad to bring it back to life还在等着你和爷爷去把他们复活使用呢3b6c77ec1b27b76ce73e154580e8332a志流新认样b059

swear 是动词 不及物动词 vi. 1.发誓;宣誓They refused to swear on a Bible. 他们拒绝手按《圣经》发誓。2.诅咒;骂脏话[(+at)]What is the woman swearing at? 那女人在咒骂什么?及物动词 vt. 1.发(誓);宣(誓)Before giving evidence you have to swear an oath. 在作证之前你得先发誓。2.发誓要;起誓保证[+to-v][+(that)]The witness swore to tell the truth. 证人起誓保证讲真话。3.【口】断言;保证说[W][Y][+(that)]He swore that he had never talked with the girl. 他发誓说,他从未与那个姑娘说过话。4.使宣誓[(+to)]You must swear him to silence. 你一定要他发誓保持沉默。5.使宣誓就任[H][(+in)]The new president will be sworn in January. 新总统将在一月份宣誓就职。6.宣誓对(罪行)作证He swore treason against his friend. 他宣誓对他的朋友犯有叛国罪作证。CUSS 即可作名词，也可作动词 。。名词 n. 1.诅咒;坏话2.古怪的家伙及物动词 vt. 不及物动词 vi. 1.乱骂;咒作动词时，CUSS 主要指咒骂 ，swear则可以宣誓、诅咒、保证等 ；

Westlife: Walk AwayAlbum:World Of Our OwnTitle:Walk AwayHere we are Just a little older Time goes by But did I ever tell you why I want you so much It came to me When you were not around You"ll always be The one to understand that"s why I can"t miss your touch All my life I waited for someone And all this time You were the one So If you want a man that is here to stay Swearing he"s forever true I"ll never walk away I"ll never give up on you And if you want a love that will save the day No matter what you"re going through I"ll never walk away I"ll never walk out on you I"ve been a fool I didn"t see behind The friend in you And baby don"t you ask me why It just couldn"t be love Cause from the start You would be the one To cure my heart But I just didn"t look this way And now I can"t get enough All my life I waited for someone And all this time You were the one, so If you want a man that is here to stay Swearing he"s forever true I"ll never walk away I"ll never give up on you And if you want a love that will save the day No matter what you"re going through I"ll never walk away I"ll never walk out on you And with a kiss you turned my world around The greatest rush of all was to be found Oh yeah You changed my whole life Coloured up the dark skies And this I promise you If you want a man that is here to stay Swearing he"s forever true I"ll never walk away I"ll never give up on you And if you want a love that will save the day No matter what you"re going through I"ll never walk away I"ll never walk out on you

The fox and the crowThere are good big very big tree in the forest, the tree lived a raven. Under the tree there is a hole, the hole lived a fox. One day, the crow flies away a piece of meat, standing on a tree to rest, the fox saw it. The fox"s mouth water, really want to get that piece of meat from the crow mouth. Due to the crow on the branch meat in mouth, the fox can"t under the tree. Eager for meat and made the fox would not give up easily. It had a turn to say: "dear crow, how are you?" The crow did not answer. The fox had to compensate with smile and said: "my dear crow, your child good?" The crow saw the fox one eye, still no answer. Fox shake tail, and a third time talked: "dear crow, your beautiful feathers, sparrows compared with you, will. That"s very kind of your throat, who loves to hear you sing, you sing a few words?" Said the crow was so satisfied: my throat good, to hear me sing but only you, fox, sang happily. Just one mouth, the meat just fell out of his mouth. Master fox meat have to drill into the hole, leaving only a crow "singing" there.Little fox and the crowThat only to be taken in by the old fox away old crow, meat is very hard to spoke to the young ravens own cheated cheated after, to the young ravens forever remember the crow family disgrace, against the may hide in a grain of intrigue. Young ravens nodded and said, "see."One day, the little crow with a piece of meat, parked in a tree and thought to myself: "if I hit the fox, you don"t fall for it."Very opportunely, then a fox came along and saw the young ravens, then walked over to the tree, playful raillery in sweet words, and now the old fox that old crow singing trick. But the young ravens in the teaching of the ancestors to ignore it.Fox some depressed, but it turned to turn eyeball, immediately in a public face, swearing: "damn little crow, you listen, the world no more bad things than your family, you are evil, who speak to you, who will be out of luck, who hear you cry, who will suffer..."Hear little fox so impudently cursing the crow family, young ravens immediately the fire emit three zhangs, "quack" open, just wanted to reprimand angrily to fox: "shut your mouth! You fox is one of the world"s most hateful guy!" But, in that instant, meat suddenly fell from the mouth to the ground, small, like the old fox, fox immediately fell to the ground meat rely on swallowed down.Fox licked mouth, infinite regret little crow on the trees and wiped to wipe your mouth, crowded a crowded eye, triumphant said: "thank you, my friend! Your vigilance is high enough, but this time you what to learn a lesson again?"

The one to understand明白我That"s why为何I can"t miss your touch不能失去你的抚慰的人All my life我的一生I waited for someone只为等待一个人And all this time而长久以来You were the one你就是唯一的人选So...所以….If you want a man that is here to stay如果你需要一个人陪伴Swearing he"s forever true我发誓将永远真诚 I"ll never walk away决不离开I"ll never give up on you决不遗弃你And if you want a love that will save the day如果你要化险为夷的爱情No matter what you"re going through无论你经历了什么I"ll never walk away决不离开你I"ll never walk out on you我决不遗弃你I"ve been a fool我曾经是个呆瓜 I didn"t see behind the friend in you看不清你内心的友善But baby don"t you ask me why宝贝别问我It just couldn"t be love为何那不可能死爱情Cos from the start因为自打一开始You would be the one to cure my heart你就是那个治疗我伤口的人Well I just be to look this way但当时我不这么想Now I can"t get enough如今我却感觉爱得不够All my life我的一生I waited for someone只为等待一个人And all this time而长久以来You were the one你就是唯一的人选So...所以….If you want a man that is here to stay如果你需要一个人陪伴Swearing he"s forever true我发誓将永远真诚 I"ll never walk away决不离开I"ll never give up on you决不遗弃你And if you want a love that will save the day如果你要化险为夷的爱情No matter what you"re going through无论你经历了什么I"ll never walk away决不离开你I"ll never walk out on you我决不遗弃你And with a kiss you turned my world around你的吻让我的世界天旋地转The greatest rush of all that"s to be found oh yeah最紧要的关头即将到来哦耶You change my whole life你改变了我的一生 Covered up the dark skies彩绘了黑色的天空And this I promise you而我将对你承诺Oh哦If you want a man that is here to stay如果你需要一个人陪伴Swearing he"s forever true我发誓将永远真诚 I"ll never walk away(never walk away) 决不离开你（决不离开你）I"ll never give up on you(I"ll never walk out on you) 决不遗弃你（决不遗弃你）And if you want a love that will save the day (saving the day) 如果你要化险为夷的爱情No matter what you"re going through无论你经历了什么I"ll never walk away(never walk away) 决不离开你（决不离开你）I"ll never walk, I"ll never walk out on you决不，决不遗弃你If you want a man that is here to stay如果你需要一个人陪伴

它的意思就是,拿细针把中指刺破,滴血到碗里,大家喝.要是喝了,大家就代表的是每个人身体里都有共同的血液,就亲如一家,就可以同生死,古代还有说法是磕头三次就可入祖坟.它代表的是一种坚定不移的决心.

1.she must has passed the driving test ,for she looks happy.（由于她看上去很高兴,她一定是通过驾驶考试了）为什么不是 drive 而是 driving? 答案解析：driving 是动名词做定语修饰后面的名词,驾驶考试,所以需要driving 2.she admitted （to）having done wrong （她承认她做错了）为什么又有ing又有过去分词?是关于不定式的语法吗? 答案解析：admit to doing sth 后面to 是介词标志, 根据动作发生在谓语之前,因此需要动名词完成形式,也就是having done ,同时前面介to 后面现在完成也需要变为having 3.i don"t like to hear children swearing （我不喜欢听孩子门发誓）为什么swear要加ing? 答案解析：上面句子结构,hear Sb doing sth , 听见某人正在说某时,现在分词doing 做宾语补足语用法 4.he usually can e up with good ideas in time of difficulty（在通常在困难的时候都会想到一个好主意/想法）he不是三单吗,为什么e不加s? 答案解析：因为句子谓语由：情态度动词+（不带to 动词不定式；也就是动词原形）所以e不能后不能加s 5.note how i do it （注意 我是怎么做这个事情的）祈使句为什么不是noting? 答案解析：其实祈使句首个单词需要用动词原形,表示一种命令,请求,省略主语第二人称 6.he ran rather than walked （他宁可跑也不走路）ran为什么不加s? 答案解析：He ran rather than walked , 都是用一般过去式,保持时态一致, ran 是 run 过去式形式 以上回答你满意么?,4,答非所问啊，不带这么混分的。。。。就你们这么搞以后不在这提问了,英语语法问题 There is a river between two hills. There is the river between two hills. 上面这两句都对吗? 经常分不清什么情况下用a,an什么情况下用THE. 书上讲的THE可以表示上一句提到过的东西,A,AN是不定冠词.总觉得实际运用中还是有好多例外啊. 比如The two children are jumping off the tree.这里为啥用THE,不用行不行. 而Some books are falling off the shelf.这句又不用the. 头晕啊谁来指点下迷津?

vow is more like 一个承诺 swear 就系发誓 vow同swear既分别就系承诺同发誓既分别 .两者意思差不多 用法就唔一样... 分别如下 swear 比较多D平时用 e.g. I swore I was telling the truth. 我发誓我说的是真相. 唔会话 I vowed I was telling the truth. 好似中文噤 平时都唔会用 "我承诺我说的是真相." 啦 随非系法庭 or etc vow 就比较 formal 系好认真既场合先用 (虽然swear都可以系认真场合用) 好似头先题过既法庭. 或者 e.g. 婚礼 They said their vows infront of the altar. 他们在圣坛作出承诺. N.B. vow 可以用做noun(名词) swear就唔可以啦 希望帮到你 =) 2008-01-12 00:14:34 补充： ah p.s. swear can also mean 讲粗口容易被人误会小心唔好用错! vow me n.[C] 1. 誓 誓言 誓约[(+of)][+to-v] We made a vow to stay friends forever. 我们发誓永远做朋友。 2. 郑重宣言;许愿 My parents are under a vow to go to church every Sunday. 我父母许愿 每星期日都去做礼拜。 3. 立誓出家;遁入空门 vt. 1. 发誓要(做)[+to-v][+(that)] I vowed never to reveal the secret. 我发誓决不泄漏这一秘密。 2. 郑重宣告[+(that)] She vowed that she would take the matter to court. 她郑重宣布要把这事诉诸法律。 vi. 1. 发誓 许愿 2. 郑重宣告 swear me vi. 1. 发誓;宣誓 They refused to swear on a Bible. 他们拒绝手按《圣经》发誓。 2. 诅咒;骂脏话[(+at)] What is the woman swearing at? 那女人在咒骂什么? vt. 1. 发(誓);宣(誓) Before giving evidence you have to swear an oath. 在作证之前你得先发誓。 2. 发誓要;起誓保证[+to-v][+(that)] The witness swore to tell the truth. 证人起誓保证讲真话。 3. 【口】断言;保证说[W][Y][+(that)] He swore that he had never talked with the girl. 他发誓说 他从未与那个姑娘说过话。 4. 使宣誓[(+to)] You must swear him to silence. 你一定要他发誓保持沉默。 5. 使宣誓就任[H][(+in)] The new president will be sworn in January. 新总统将在一月份宣誓就职。 6. 宣誓对(罪行)作证 He swore treason against his friend. 他宣誓对他的朋友犯有叛国罪作证。

是不是swear

The song:Nothing belongs to The script.

1、绝不同意为了成功而不择手段，刻薄成家，理无久享。 Never agree that in order to succeed without resorting to any means, be mean to start a family and enjoy it for a long time. 2、让磨难打掉脆弱的铁屑，铸成锋利的钢刀。 Let hardships break away fragile iron chips and cast sharp steel knives. 3、在顺境中执着，在逆境中沉着。 Persistence in prosperity and posure in adversity. 4、你失去了金钱，可以再挣；你失去了一生，便再也不能回头。 If you lose money, you can earn it again; if you lose your life, you can never go back. 5、青春要放肆一点，微笑要灿烂一些。 Youth should be bold and *** ile more brilliantly. 6、苦于现在，甜与未来，没有克服不了的困难。 Suffering from the present, the sweet and the future, there are no insurmountable difficulties. 7、即使上帝也无法改变过去。 Even God can"t change the past. 8、只知刚的人，难免会被折断；只有柔的人，到头来终是懦夫。 A man who knows nothing but righteousness will inevitably be broken; a man who knows nothing but gentleness will be a coward in the end. 9、伪装的善良比真实的凶残更可怕。 Kindness in disguise is more terrible than real cruelty. 10、行路人，用足音代替叹息吧！ Pedestrians, substitute footnotes for sighs. 11、强者不是没有眼泪，只是可以含着眼泪向前奔跑。 The strong is not without tears, but can run forward with tears. 12、在所有的批评家中，最伟大最正确最天才的是时间。 Of all the critics, the greatest, the most correct and the most genius is time. 13、我们可以没钱，可以没事业，但就不能没信心。 We can have no money, no career, but we can"t lose confidence. 14、既靠天，也靠地，还靠自己。 It depends not only on heaven, but also on the earth, and on oneself. 15、把感恩刻在石头上，把仇恨写在沙滩上。 Gratitude is engraved on stone and hatred on the beach. 16、无论梦想有多么疯狂，都要试着去接近它。 No matter how crazy the dream is, try to get close to it. 17、凡百事之成也在敬之，其败也必在慢之。 Every success of Pepsi is respected, and its failure will also slow down. 18、哪儿有勤奋，哪儿就有成功。 Where there is diligence, there is success. 19、爱其子而不教，犹为不爱也；教而不以善，犹为不教也。 If you love your son but don"t teach him, you still don"t love him; if you teach him but don"t teach him good, you still don"t teach him. 20、人类被赋予了一种工作，那就是精神的成长。 Mankind has been given a job, that is, spiritual growth. 21、人生最大的错误是不断担心会犯错。 The greatest mistake in life is to constantly worry about making mistakes. 22、用宽容的心征服别人无往不胜。 Conquer others with tolerance. 23、拼搏的汗水，能浇开理想的花朵。 The sweat of struggle can water the ideal flowers. 24、放手一博去创业，不要给你的人生留下遗憾。 Let go of a blog to start a business, don"t leave regrets for your life. 25、永远不要跟任何人解释你自己。 Never explain yourself to anyone. 26、为未来投资的人是对现实忠实的人。 People who invest in the future are loyal to reality. 27、学习就是等于给一个普通人装备上武器，而不是大船上的瞄。 Learning means equipping an ordinary person with weapons, not aiming at a large ship. 28、生命不等于是呼吸，生命是活动。 Life is not breathing, life is activity. 29、骆驼走得慢，但终能走到目的地。 Camels walk slowly, but they eventually reach their destination. 30、路不该是疑问句，要比谁都清晰。 The road should not be an interrogative sentence, but clearer than anyone else. 31、你要以乐观的态度看待这个世界，你会发现世界是如此得美好！ If you look at the world with optimi *** , you will find that the world is so beautiful! 32、经常不断地学习，你就什么都知道。你知道得越多，你就越有力量。 Keep learning and you will know everything. The more you know, the more powerful you are. 33、让我们在热血沸腾的年代，奏响青春旋律。 Let"s play the youth melody in the age of boiling blood. 34、人若软弱就是自己最大的敌人；人若勇敢就是自己最好的朋友。 If a man is weak, he is his greatest enemy; if he is brave, he is his best friend. 35、有时你的梦想达到是一种幸福，有时梦想破灭也是一种幸福。 Sometimes your dream is a kind of happiness, sometimes it is a kind of happiness that your dream is broken. 36、希望，治疗人间百疾的良药，它即便宜又有效。 Hope, the good medicine for all kinds of human diseases, it is even appropriate and effective. 37、人生在世，应该这样，在芬芳别人的同时美丽自己。 Life, should be so, while fragrant others at the same time beautiful themselves. 38、只有脚踏实地的人，才能够说：路，就在我的脚下。 Only a down-to-earth person can say: the road is under my feet. 39、相信别人，放弃自己，这是许多人失败人生的开始！ Believe in others, give up oneself, this is the beginning of many people"s failed life! 40、青春到底是什么，你问自己这个问题，却一直没有得到答案。 What is youth? You ask yourself this question, but you never get the answer. 41、研究碰到困难，别退缩，相信自己，一定能行！ Don"t flinch from difficulties in research. Believe in yourself, you can do it! 42、诸恶莫做，众善奉行，莫以善小而不为，莫以恶小而为之。 Don"t do evil, do good, don"t do good in a *** all way, don"t do evil in a *** all way. 43、与其当一辈子乌鸦，莫如当一次鹰。 It is better to be an eagle than to be a crow all one"s life. 44、伟大的作品不只是靠力量完成，更是靠坚定不移的信念。 Great works are acplished not only by strength, but also by unswerving faith. 45、每天告诉自己一次，“我真的很不错”。 Tell yourself once a day, "I"m really good." 46、彩虹总在风雨后，阳光总在乌云后，成功总在失败后。 Rainbows are always after storms, sunshine is always after dark clouds, and success is always after failure. 47、只要认真学习，就一定会有进步。 As long as we study hard, we will make progress. 48、我甘心用十分耕耘，换取哪怕一分收获，为了梦，一切都值得。 I am willing to work very hard, in exchange for even a harvest, in order to dream, everything is worth it. 49、人生不是受环境的支配，而是受自己习惯思想的恐吓。 Life is not dominated by the environment, but by the intimidation of their habitual thinking. 50、莫向不幸屈服，应该更大胆、更积极地向不幸挑战！ Don"t give in to misfortune, we should be bolder and more active to challenge misfortune! 51、目标和信念是战胜困难和不幸的利剑，可以使人发挥出超常的潜能。 Goals and beliefs are powerful swords to overe difficulties and misfortunes and enable people to develop their extraordinary potential. 52、爱情是一种幸福的体验，而不应该是担心和煎熬。 Love is a happy experience, not a worry and suffering. 53、生活可以漂泊，可以孤独，但灵魂必须有所归依。 Life can be wandering, lonely, but the soul must have some dependence. 54、一个人炫耀什么，说明内心缺少什么。 What a person shows off shows what he lacks in his heart. 55、我们的生命必须开花。道德无私心就是人生之花。 Our lives must blossom. Moral selflessness is the flower of life. 56、在人之上要自重，在人之下要自尊。 Be self-respectful above and below. 57、目标不是都能达到的，但它可以作为瞄准点。 Targets are not always achievable, but they can be used as aiming points. 58、树的方向，由人决定，人的方向，自己决定。 The direction of the tree is decided by man, and the direction of man is decided by himself. 59、不懈奋斗，生命才有辉煌；努力学习，思想才有灵光。 Only by unremitting struggle can life be brilliant; only by studying hard can ideas be brilliant. 60、失败的尽头是成功，努力的终点是辉煌。 The end of failure is success, and the end of effort is glory. 61、用自己最大的努力，要自己最小的满足。 With their best efforts, to their *** allest satisfaction. 62、骄傲是胜利下的蛋，孵出来的却是失败。 Pride is the egg of victory, but failure hatches. 63、愚痴的人，一直想要别人了解他。有智慧的人，却努力地了解自己。 A fool always wants others to know him. A wise man tries to understand himself. 64、你可以选择这样的“三心二意”：信心、恒心、决心；创意、乐意。 You can choose to be ambitious: confidence, perseverance and determination; creativity and willingness. 65、在我们所具有的一切缺点中，最为粗鲁的乃是轻视我们的存在。 Of all our shortings, the most rude is to despise our existence. 66、读一切好的书，就是和许多高尚的人说话。 Reading all good books is talking to many noble people. 67、与其脸红脖子的骂人，不如有用优雅的方式，看起来比较不失礼。 Instead of blushing and swearing, it"s better to use elegant ways to look more polite. 68、世上没有一件工作不辛苦，没有一处人事不复杂。 There is no place in the world where work is not hard and personnel are not plicated. 69、对书本知识只有自觉的推敲，才能透过书的词语而获得内在的精力。 Only by consciously deliberating on the knowledge of books can we acquire the inner energy through the words of books.

1.不画大饼，永远真心，真心没错，错的是人，所有的爱都可贵，希望都可以和真诚的人硬碰硬，不会和差点的人周旋。 Don"t draw big cakes, always be sincere, really right, wrong is people, all love is valuable, I hope that I can meet hard with sincere people, and will not deal with people who are almost. 2.情绪的尽头，不是脏话，不是发泄，是沉默。The end of emotions is not swearing, not venting, but silence. 3.有人为了睡去骗爱，有人为了钱去出卖，而我只对你真诚，希望你也是。 Some people cheat love for sleep, some people sell for money, and I am only sincere to you, I hope you are the same. 4.你见证我的发型换了又换，从幼嫩到慢慢长大，从恋爱到分手，安慰和出主意，没人能替代你。 You witnessed that my hair style changed and changed, from young to growing up slowly, from love to breaking up, comforting and giving ideas, no one can replace you. 5.虽然我满口脏话手里掐着烟，但我也能把温柔的一面给你。 Although I am full of dirty words and smoke in my hand, I can also give you the gentle side. 6.可惜世上没有反方向的钟，这交替的四季，新年都在告诫你，不要回头，所有受过的委屈都在提醒你要好好争气。 Unfortunately, there is no clock in the opposite direction in the world. In this alternating four seasons and New Year, you are warned not to look back. All the grievances you have suffered remind you to live up to expectations. 7.当你觉得累的时候就靠在我的身上吧，我是说挫折苦难的时候，我陪你，只要你对我真心。When you feel tired, lean on me. I mean, when you are frustrated and suffering, I will accompany you as long as you are sincere to me. 8.平底鞋给不了高跟鞋的骄傲，高跟鞋也给不了平底鞋的安全感。 Flat shoes can"t give high heels pride, and high heels can"t give flat shoes a sense of security. 9.总忙着给别人解释必定会忘掉自己是什么位置，喜欢沉默不代表我软弱，就像喜欢和平，但我从不畏惧战争。 Always busy explaining to others will definitely forget where I am. Like silence does not mean that I am weak, just like I like peace, but I am never afraid of war. 10.你现在身边的人可能是你今后日思夜想都见不到的人。 The people around you now may be people you won"t see day and night in the future.

英语作文《how to develop good habits》：Develop good behavior habits and control your mouth.Some students speak freely, swearing and swearing come out at random, which makes people extremely disgusted and hurts the harmony of students.Some students do not pay attention to protecting the environment and often spit everywhere, which affects indoor hygiene. It is easy to infect diseases in crowded classrooms.Some students behave even worse. They practice "shooting" with spittle from upstairs at the "enemy" below.How bad these behaviors are. I advise all students to control their mouths and let our mouths really become a window to publicize our civilized image.译文：养成良好的行为习惯要管住自己的口。有的同学说话口无遮拦，脏话、粗话随口而出，让人听了极为反感，也伤了同学的和气。有的同学不注意保护环境，经常随地吐痰，影响室内卫生不说，在人数拥挤的教室里还容易传染疾病。还有的同学行为更糟糕，从楼上对准下面的“敌人”练习用唾沫“射击”。这些行为是多么的不好，我奉劝所有的同学都管住自己的口，让我们的嘴巴真正成为宣传我们文明形象的窗口。

1. 以“on change”为题,写一篇作文 范文两篇：How I Change My life has changed a lot in the past three years.When I was in Grade 7,I was a short and shy girl with long hair.I often listen to music at home.But now I like to make friends and take part in all kinds of activities.I prefer running with my clas *** ates.So I"m being much healthier than before.Not only my parents but also my teachers are satisfied with me. How I Change My life has changed a lot in the past three years.I was fat and short when I came to the middle school.At that time I was interested in puter games and I spent most of time on it.My parents were worried about me. Now,I like sports and I often play football with my clas *** ates after school.So I bee taller and stronger than before.I like reading,too.I usually go to the library to read some interesting books.Good habits help me to study better and keep healthier. 2. my life change为题写作文 Change in my life With the development of the economy.lots of things changed in my life . For example,the way go to school.We went to school by bus ereryday before,but now we always go to school by subway.It easier and faster .For this ,we don"t have to get up so early .And there are so many different subjects can choose.We can also join the physical activities.Such as Taekwondo ,Karate,and so on. There are so many changes in my life . Do you see it? 3. 用How I Change写英语作文 How can i change myself? Today i made a mistake.Because this is my first time to deal with the shipping document after my back,and meet some problem,so i didn"t check it with my leader,and then i made it.But sometimes i confused,somebody who has already deal with it for one year,he should know how to deal with the case like that,he didn"t mention,just said don"t know.Really strange.So now the trouble work here is they just do the work without thinking ,just like what our boss told us.And this is still his headache point.How can lead the other people thinking well before they do the work?I have no idea either.Just say good luck,Eliza,and try to do if you can do. 4. 用changes to me 写一篇文章 How time flies! It has been o years since I came to XXX Middle school. In the o years, many things have happened. I also meet a lot of friends here. Two years is not too long for man"s life. But the o years I lived on campus has changed me a lot. For example, from the day I was born to the day I went to school, I had never left home alone for a long time and do housework by myself. How to deal with my roommates and clas *** ates? These questions were all difficult for me to work out at that time. Today I get on well with them and do everything in life calmly. But all these changes were not easy for me. Sometimes I feel lonely, angry and also homesick. But fortunately I meet a lot of good friends. They helped me spend these sad days. I find words cannot express my sincere thanks to them. When they need help, I will do everything I can to help those who helped me before. 5. 作文《My change》 Now,I am a junior 3 student,and I have changed a lot.My biggest change is that I don"t have enough time anymore.When I was in grade 7,I used to have a little homework to do and I could have a lot of time to chat with friends.But now,even though I do homework or exercises all the time ,I can"t find much free time.In the evening,I used to play the piano and watch TV after I go back home,but now I have to study and read books till very late.Although I am very busy now,I like the busy junior 3 school life.Another change is that I used to be very crazy and wild,but now I"m quiet and friendly,so although I am busy getting ready for next year"s entrance exam to high school,I am happy and feel good about my progress.现在，我是一名初中三学生，我已经发生了很大的变化.我国最大的变化是，我没有足够的时间了.当我在7年级，我曾经有一点功课要做，我可以有很多时间来与朋友聊天.但现在，即使我做功课或演习的时间，我找不到多少空闲时间.晚上，我用弹钢琴和收看电视节目后，我回家，但现在我必须学习和读书到很晚.虽然我现在很忙，我喜欢忙碌的初中3学校生活.另一个变化是，我曾经非常疯狂和野生，但是现在我安静的，友好的，所以虽然我忙着准备明年的高考到高中，我很高兴和感觉很好，我的进步.。 6. 以Changes写一篇英语作文,不少于50词 How time flies ! One years passed by . I"m 12 years old . I think i change a lot in these years . For example , i begin to like studying math . And my hair was long and blank . What is the most , i put on weight . Sometimes , i think i look like a panda . In all , i begin to understand my parents why they always ask me to do more homework . In fact , they hope me have a good future.。 7. 《My change》英语作文 Change has been the one constant in my life. While staring out at the bleak Wisconsin winter, I think back to my beginnings on a warm tropical island. The biggest change was probably the first — moving from that buzzing Spanish-speaking isle to the sleepy sea-side town that was Tampa in 1978. It took me some time to realize that the other pre-schoolers could not understand my native tongue. Before long, I too was speaking their language.Five years later I, an excited eight-year-old girl, boarded a school bus in New Jersey. The excitement quickly turned to fear as I heard rampant swearing in the back of the bus. I was truly shocked when the bus driver did nothing to stop the vulgarity. In my schools in Florida such behavior would have met with a bar of soap and a visit to the principal"s office. A year later, I had a "Jersey" accent, and had started swearing too.After nine years my family then moved to a place called "a whole "nother country": Texas. I discovered that everything is bigger in Texas, from the size of a glass of ice tea to the distances on the road. My mother added barbecued brisket to the regular menu of turkey and Idaho potatoes on Monday and arroz con pollo on Tuesday. The incredibly friendly Texans, wearing cowboy boots and going to high school football games on Friday nights, seemed a totally different breed from my friends in New Jersey. A slight drawl entered my speech.In o years time, I found myself in the mountains of rural Bolivian. As part of a team of doctors and students researching hypertension on a group of African- Bolivian villagers, I quickly learned a new vocabulary that included medical and anthropological terms. The greatest test of my linguistic abilities came when a villager accused me of drinking blood samples in some kind of vampire-like witchcraft ritual. I had to bridge a vast cultural gulf to explain a DNA isolation and *** ysis protocol in Spanish to someone who had never heard of a gene much less a double helix.A year later I stood in a line at a McDonalds outside Buenos Aires asking for a sorbeto with a Puerto Rican accent and receiving a blank stare in return. I did not realize that in Argentina the word for straw was papote. Working at the U.S. embassy, I could clearly see the obvious differences beeen the U.S. and Argentina, but being out among the people and actually experiencing the culture helped me begin to understand and appreciate the subtle differences which, when taken together, make up a people.Each place I have lived has its differences, from the obvious distinctions of Wisconsin and Texas weather, to the regional variations of the Spanish language. I bring with me wherever I go a part of those places and the impact they have had on my life, most evident to others by the variations in my speech. Beneath all the accents, however, lies something more significant, for I believe who you are is immeasurable more important than where you were. When I was younger, I could not clearly discern beeen situations where I should or should not adopt the ways of those around me. With maturity however I have e to understand the crucial difference beeen adaptation and assimilation. I have chosen to reject the vulgarity of the New Jersey school bus; I have also adopted the Texans" warm and friendly manner. Having experienced frequent moves to very different surroundings, I can adapt without promising what is important to me while learning from each new setting.A sign hung in my garage for many years that said, "Home is where you can scratch where it itches." To me this means that home is wherever you are fortable and secure with yourself and your surroundings. I will be at home and prepared to meet new challenges wherever I am. Starting over so many times has taught me not to fear failure, but rather to embrace opportunities for change。 8. 用“We Have Changed”怎么写作文 One"s life will change, just like a butterfly and silkworm, a second transformation we grew up, we bee more sensible every day, bee more abundant. New knowledge every day long, but each is progress. From *** all to large, I have many dreams. When I don"t want to grow up, and friends can play, but I"m still a strength to grow. Now, I"m not six or seven years old baby, but every day looking forward to grow up. Because my ideal: growing up as a writer, as an architect, when the chairman of the board, or when a politician。. my ideal, be the most changeful. Now, I want to fight for the politicians goal! Grow up to serve the people, be a good servant of the people. In order to achieve the ideal, I"m not enrich, improve their. Now of I, study harder and harder, getting good grades. When calculating the time, never from and start, and now the brain with the "robot" brains similar, to the subject are hard to do well and quickly. I used the independence of the very poor. Now, I can one homework, read a book and thinking. Now I understand, not to buy toys, put the money in the bank, grew up reading use. I used to have a lot of hobbies, paper-cut, chess, painting, swimming。 Now. My hobby is still many, but also have a new hobby - playing the clari. The original active again and again I quiet lost themselves in the beautiful music. Yes, each person"s life have a lot of change, dreams change, changing tastes, personality changes, learning change。.. The long road of life, every change to let others see -- I really grown up!!

1、年轻的英语是young，音标英[j??]、美[j??]。2、adj.年轻的；初期的；没有经验的3、n.青年人；幼崽4、比较级:younger最高级:youngest名词:youngness5、young的基本意思是“年轻的，年幼的，幼小的，新发展的”，指某人或某物处于一种不成熟的或萌芽的状态。用于比喻可指“有朝气的，有青春活力的”。6、例句：Thoseclothessheswearingaremuchtooyoungforher.7、她穿的那些衣服显得太年轻了。

The Outsiders s e hintonThey walked out slowly, silently, smiling."Need a haircut, greaser?" The medium-sized blond pulled a knife out of his back pocket and flipped the blade open.I finally thought of something to say. "No." I was backing up, away from that knife. Of course I backed right into one of them. They had me down in a second. I fought to get loose, and almost did for a second; then they tightened up on me and slugged me a couple of times. So I lay still, swearing at them between gasps. A blade was held against my throat."How"d you like that haircut to begin just below the chin?" Synopsis:According to Ponyboy, there are two kinds of people in the world: greasers and socs. A soc (short for "social") has money, can get away with just about anything, and has an attitude longer than a limousine. A greaser, on the other hand, always lives on the outside and needs to watch his back. Ponyboy is a greaser, and he"s always been proud of it, even willing to rumble against a gang of socs for the sake of his fellow greasers--until one terrible night when his friend Johnny kills a soc. The murder gets under Ponyboy"s skin, causing his world to crumble and teaching him that pain feels the same whether a soc or a greaser.S.E. Hinton on the book:The Outsiders is definitely my best selling book; but what I like most about it is how it has taught a lot of kids to enjoy reading.Publishing Information:First Published: 1967Published by: Puffin BooksAwards:New York Herald Tribune Best Teenage Books List, 1967Chicago Tribune Book World Spring Book Festival Honor Book, 1967Media and Methods Maxi Award, 1975ALA Best Young Adult Books, 1975Massachusetts Children Book Award, 1979 因为我自己没有还过,不知道你要的是不是这篇如果不是可以通知我,帮你再找

1、咱们不喜欢他了好不好，不委屈了好不好。 Let"s not like him. Let"s not be aggrieved. 2、故事我忘了，你，我也不要了。 I forgot the story. I don"t want you. 3、看住时间，别让它再流浪，从前我太适应悲伤。 Watch the time, don"t let it wander again, I used to be too used to sadness. 4、幸福不是得你所想，而是想你所得！ Happiness is not what you think, but what you get! 5、承诺是卑微的，心痛确是永久的。 Commitment is humble, heartache is permanent. 6、只有装作无所谓，才能显得不那么卑微。 Only by pretending that it doesn"t matter, can it be less humble. 7、我庆幸遇见你，却遗憾只是遇见你。 I"m glad to meet you, but I"m sorry to just meet you. 8、我会伤心会难过，只是你从来都不知道。 I will be sad, but you never know. 9、我喜欢你是寂静的，仿佛你消失了一样。 I like you are silent, as if you disappeared. 10、大家都离开了，去向他方！我们都舍去了好多！ Everybody"s gone to the other side! We all gave up a lot! 11、说一句不争了好累，可是倒下后又能依靠谁。 I"m tired, but who can I rely on after I fall down. 12、别总说我假正经，我禽兽的一面你敢接受吗。 Don"t always say I"m serious. Do you dare to accept my animal side. 13、黑名单里的那个人，是我最爱的人。 The person on the blacklist is my favorite. 14、我们互相戴错了面具，于是还未相遇便匆匆失散。 We put on the wrong mask to each other, so we were separated in a hurry before we met. 15、我没有停止爱你，我只是决定不再表现出来。 I didn"t stop loving you, I just decided not to show it. 16、只要不去想，肤浅的快乐其实很容易。 As long as you don"t think about it, superficial happiness is easy. 17、我相信我爱你。依然。始终。永远。 I believe I love you. Still. throughout. Forever. 18、总有一些人在今生里刻骨铭心，却又无奈的错过。 There are always some people in this life unforgettable, but helpless to miss. 19、给我三年时间，三年后想起你绝不会再这么痛。 Give me three years, three years later I think of you will never be so painful. 20、我只是不小心闹了，你就不再对我笑了。 I"m just careless, you don"t laugh at me anymore. 21、不出意外的话，我们这辈子应该不会再见了。 If there"s no accident, we"ll never see each other again. 22、你去做她的盖世英雄吧，我可是混世大魔王。 You go to be her hero. I"m the devil. 23、在光明和黑暗的斗争中，你们选了黑暗。 In the struggle between light and darkness, you chose darkness. 24、爱总是让人哭，让人不满足。 Love always makes people cry and unsatisfied. 25、从小到大，唯一不变的就是那颗不爱读书的心。 From small to large, the only constant is the heart that does not love reading. 26、如果我不变的更好，我拿什么要你后悔。 If I don"t change better, what can I do for you to regret. 27、人要走，千万种理由。 There are thousands of reasons for people to leave. 28、我爱大风和烈酒，却被孤独割破了手。 I love the wind and the liquor, but my hand is cut by loneliness. 29、没有难过，没有失望，也不是很快乐。 Not sad, not disappointed, not very happy. 30、全世界都背叛了你，我也会在你身边。 The whole world has betrayed you, and I will be by your side. 31、等待，是一生最初的苍老。 Waiting is the first aging of life. 32、流落的红尘，是爱用泪水刻写下的伤痕。 The world of mortals, is love with tears carved down the scars. 33、忘了自己曾经多美好，不会脏话，不会笑里藏刀。 Forget how good they used to be, no swearing, no laughing. 34、最后你幸福终老，我泪流满面，却依旧拍手叫好。 In the end, you will be happy and I will cry, but I still applaud. 35、催泪的情书是，你我的聊天记录。 Tearful love letters are the chat records of you and me. 36、越是用尽力气的感情，越是脆弱。 The more exhausted the feelings, the more vulnerable. 37、有时候你远在天边，有时候你近在眼前。 Sometimes you are far away, sometimes you are near. 38、时间不是让人忘了痛，而是让人习惯了痛。 Time is not to let people forget the pain, but to let people get used to the pain. 39、你就像掉进厕所的手机，不要可惜，要了恶心。 You"re like a cell phone falling into the toilet. Don"t be a pity. It"s disgusting. 40、你自由了，把快乐还给我吧。 You"re free. Give me back my happiness. 41、每失一物，必有所得；每得一物，必有所失。 For every thing lost, there is gain; for every thing gained, there is loss. 42、时间是最好的良药，是我吃错药才没有成效。 Time is the best medicine, I take the wrong medicine is not effective. 43、你不知道吧，每一次巧合里都有我的努力。 You don"t know, every coincidence has my efforts. 44、我爱你，爱已深入骨髓，你要我如何割舍。 I love you, love has been deep into the bone marrow, how do you want me to give up. 45、今生都等不来，何必寄往来生。 This life can"t wait, why send it to the next life. 46、尽管我知道结局，明天醒来我还是会喜欢你。 Even though I know the ending, I will like you when I wake up tomorrow. 47、我可以爱迩撕心裂肺，也可以走得干干脆脆。 I can love you, or I can go dry and crisp. 48、我能没有你，就算是我施舍她的可不可以？ Can I give to her without you? 49、深情从来都只有被辜负，只有薄情才会被记住。 Deep love has always been only let down, only love will be remembered. 50、给我一个理由来忘记，一直那么爱你的我。 Give me a reason to forget that I always love you.

题目哪。。。。。。。。。

SONNET #1 　　by: William Shakespeare 　　FROM fairest creatures we desire increase, 　　That thereby beauty"s rose might never die, 　　But as the riper should by time decease, 　　His tender heir might bear his memory; 　　But thou, contracted to thine own bright eyes, 　　Feed"st thy light"s flame with self-substantial fuel, 　　Making a famine where abundance lies, 　　Thyself thy foe, to thy sweet self too cruel. 　　Thout that are now the world"s fresh ornament 　　And only herald to the gaudy spring, 　　Within thine own bud buriest thy content 　　And, tender churl, mak"st waste in niggarding. 　　Pity the world, or else this glutton be, 　　To eat the world"s due, by the grave and thee. 一 　　对天生的尤物我们要求蕃盛， 　　以便美的玫瑰永远不会枯死， 　　但开透的花朵既要及时凋零， 　　就应把记忆交给娇嫩的后嗣； 　　但你，只和你自己的明眸定情， 　　把自己当燃料喂养眼中的火焰， 　　和自己作对，待自己未免太狠， 　　把一片丰沃的土地变成荒田。 　　你现在是大地的清新的点缀， 　　又是锦绣阳春的唯一的前锋， 　　为什么把富源葬送在嫩蕊里， 　　温柔的鄙夫，要吝啬，反而浪用？ 　　可怜这个世界吧，要不然，贪夫， 　　就吞噬世界的份，由你和坟墓。

　　BUT wherefore do not you a mightier way　　Make war upon this bloody tyrant, Time?　　And fortify yourself in your decay　　With means more blessèd than my barren rime?　　Now stand you on the top of happy hours,　　And many maiden gardens, yet unset,　　With virtuous wish would bear your living flowers,　　Much liker than your painted counterfeit:　　So should the lines of life that life repair　　Which this time"s pencil or my pupil pen,　　Neither in inward worth nor outward fair　　Can make you live yourself in eyes of men.　　To give away yourself keeps yourself still,　　And you must live, drawn by your own sweet skill.　　但是为什么不用更凶的法子　　去抵抗这血淋淋的魔王--时光？　　不用比我的枯笔吉利的武器，　　去防御你的衰朽，把自己加强？　　你现在站在黄金时辰的绝顶，　　许多少女的花园，还未经播种，　　贞洁地切盼你那绚烂的群英，　　比你的画像更酷肖你的真容：　　只有生命的线能把生命重描；　　时光的画笔，或者我这枝弱管，　　无论内心的美或外貌的姣好，　　都不能使你在人们眼前活现。　　献出你自己依然保有你自己，　　而你得活着，靠你自己的妙笔。　　I was her she was me 我就是她 她就是我　　We were one we were free 我们是一个人 我们很自由　　And if there"s somebody calling me on 如果有人在呼唤我　　She"s the one 她就是那个人　　If there"s somebody calling me on 如果有人呼唤我　　She"s the one 那一定是她　　We were young we were wrong 我们很年轻 我们曾做过错事　　We were fine all along 我们一直都很好　　If there"s somebody calling me on 如果有人呼唤我　　She"s the one 她就是那个人　　When you get to where you wanna go 当你去了你想去的地方　　And you know the things you wanna know 你知道了你想知道的事情　　You"re smiling 你会开心的笑　　When you said what you wanna say 当你说出你要说的　　And you know the way you wanna play 按你喜欢的方式做事　　You"ll be so high you"ll be flying 你会感觉你在飞翔　　Though the sea will be strong 尽管困难重重　　I know we"ll carry on 我知道我会继续努力的　　Cos if there"s somebody calling me on 因为如果有人呼唤我　　She"s the one 她就是那个人　　If there"s somebody calling me on 如果有人呼唤我　　She"s the one 她就是那个人

这里by far是一个短语。意思是远远超越。outstrip的宾语是anything achieved by our distant ancestors。所以翻译成蚂蚁远远超越了人类的祖先

Eminem<Sing For The MomentThese ideas are nightmares for white parents Whose worst fear is a child with dyed hair and who likes earrings Like whatever they say has no bearing It"s so scary in a house that allows no swearing To see him walkin" around wit his headphones blaring Alone in his own zone, cold and he don"t care He"s a problem child, what bothers him all comes out When he talks about his ****in" dad walkin" out "Cuz he hates him so bad that he blocks him out "Cuz he hates him so bad that he blocks him out But if he ever saw him again, he"d prolly knock him out His thoughts are whacked, he"s mad so he"s talkin" back Talkin" black, brainwashed from rock and rap He sags his pants, dew-rags and a stockin" cap His stepfather hit him so he socked him back And broke his nose, this house is a broken home There"s no control, he just lets his emotions go- C`mon! Sing with me (Sing!), sing for the year (Sing It!) Sing for the laughter, sing for the tear (C`mon!) Sing it with me, just for today Maybe tomorrow, the good Lord will take you away Entertainment is changin", intertwinin" with gangstas In the land of the killers, a sinner"s mind is a sanctum Only ya unholy, only have one homey Only this gun, lonely, "cuz don"t anyone know me But everybody just feels like they can relate I guess words are a mother****a, they can be great Or they can degrade, or even worse, they can teach hate It"s like kids hang on every single statement we make Like they worship us, plus all the stores ship us platinum Now how the **** did this metamorphosis happen? From standin" on corners and porches just rappin" To havin" a fortune, no more kissin" ass But then these critics crucify you, journalists try to burn you Fans turn on you, attorneys all wanna turn at you To get their hands on every dime you have They want you to lose your mind every time you mad So they can try to make you out to look like a loose cannon Any dispute, don"t hesitate to produce handguns Thats why these prosecutors wanna convict me Strictly just to get me offa these streets quickly But all their kids been listen"n to me religiously So I"m signin" CDs while police fingerprint me They"re for the judge"s daughter, but his grudge is against me If I"m such a ****in" menace, this shit doesn"t make sense B It"s all political, if my music is literal and I"m a criminal, How the FUCK can I raise a little girl? I couldn"t, I wouldn"t be fit to You"re full of shit too, Guerrera, THAT WAS A FIST THAT HIT YOU! C`mon! Sing with me (Sing!), sing for the year (Sing It!) Sing for the laughter, sing for the tear (C`mon!) Sing it with me, just for today Maybe tomorrow, the good Lord will take you away They say music can alter moods and talk to you But can it load a gun for you and cock it too? Well if it can, then the next time you assault a dude Just tell the judge it was my fault, and I"ll get sued See what these kids do, is hear about us totin" pistols And they wanna get one, "cuz they think the shit"s cool Not knowin" we"re really just protectin" ourselves We entertainas, of course this shit"s affectin" our sales You ignoramus, but music is reflection of self We just explain it, and then we get our checks in the mail It"s ****ed up ain"t it, how we can come from practically nothin" To bein" able to have any ****in" thing that we wanted That"s why we sing for these kids that don"t have a thing Except for a dream and a ****in" rap magazine Who post pin-up pictures on their walls all day long Idolize their favorite rappers and know all they songs Or for anyone who"s ever been through shit in they lives So they sit and they cry at night, wishin they die Till they throw on a rap record, and they sit and they vibe We"re nothin" to you, but we the ****in" shit in they eyes That"s why we sieze the moment, try to freeze it and own it Squeeze it and hold it, "cuz we consider these minutes golden And maybe they"ll admit it when we"re gone Just let our spirits live on, through our lyrics that you hear in our songs And we can- C`mon! Sing with me (Sing!), sing for the year (Sing It!) Sing for the laughter, sing for the tear (C`mon!) Sing it with me, just for today Maybe tomorrow, the good Lord will take you away C`mon! Sing with me (Sing!), sing for the year (Sing It!) Sing for the laughter, sing for the tear (C`mon!) Sing it with me, just for today Maybe tomorrow, the good Lord will take you away

问题一：这种原理图是什么意思？ 各支路通过接地母线集中一点接地。 问题二：工作原理是什么意思 工作原理就是 工作的基本规律。多指事物运行的原由或者规律。 原理：自然科学和社会科学中具有普遍意义的基本规律。是在大量观察、实践的基础上，经过归纳、概括而得出的。既能指导实践，又必须经受实践的检验。 问题三：电气原理图中的W是什么意思 这个是辅助控制电源电路图。进线一个施耐德C65H 2P D16的微断加辅助触点，接DG-1KVA 380/220V的变压器。变压器下口是施耐德C65N 2P C6的微断。前面的微断是起保护作用（MCB）所以画上了线圈 后面的起开关作用没有画线圈。（CP） 21A1是箱号，CPT是元件缩写变压器，其他的类似。 问题四：硬件原理图，图中标出来的是什么意思？？ 15分 我可以负责地告诉你，这里99.9999999%都是电脑用户，有的高端点，有的废材点，也有明明废材还硬装高端的。但绝对没有懂你问的这些玩意的，你别把电脑用户和硬件、电路工程师弄混了！！ 问题五：PCB电路原理图中的几个英文缩写是什么意思？ 应该是网络名，这个要结合具体应用理解，单词直接翻译是窗口下，灯开关 问题六：基本原理是什么意思 基本原理是一种机器发明或运行，或某些事物存在所遵循的自然条件自然规律为前提的。 比如：孔明灯、热气球建造的基本原理是空气受热会上升；直升机起飞的基本原理是向下方旋打空气获得向上的反盯力；变压器的基本原理是UI=U"I" 即变压器两边的电功率相等；人造卫星的基本原理是GMm/r2=mv2/R ,即；地球的万有引力提供卫星的向心力。等等。能不能在问的具体点，好针对性回答 问题七：电气原理图上的SW是什么意思？ 电气原理图上的SW是开关的意思。 开关是指一个可以使电路开路、使电流中断或使其流到其他电路的电子元件。最常见的开关是让人操作的机电设备，其中有一个或数个电子接点。接点的“闭合”（closed）表示电子接点导通，允许电流流过；开关的“开路”（open）表示电子接点不导通形成开路，不允许电流流过。 最简单的开关有二片名叫“触点”的金属，二触点接触时使电流形成回路，二触点不接触时电流开路。选用接点金属时需考虑其对抗腐蚀的程度，因为大多数金属氧化后会形成绝缘的氧化物，使接点无法正常工作。选用接点金属也需考虑其电导率、硬度、机械强度、成本及是否有毒等因素。有时会在接点上电镀抗腐蚀金属。一般会镀在接点的接触面，以避免因氧化物而影响其性能。有时接触面也会使用非金属的导电材料，如导电塑胶。开关中除了接点之外，也会有可动件使接点导通或不导通，开关可依可动件的不同为分为杠杆开关（Toggle switch）、按键开关、船型开关（Rocker switch）等，而可动件也可以是其他型式的机械连杆。 问题八：普遍原理是什么意思？ 原理是名词，有两方面的意思：1.带有普遍性的、最基本的、可以作为其他规律的基础的规律；2.具有普遍意义的道理。 问题九：VDD在电路原理图里是什么意思？如图 GND为公共接地，VDD则为供电电压，简单的电路只有一个供IC和其他部分工作的恒定电压，一般为5V 3.3V等，复杂点的电路有好几个不同值的VDD1 VDD2 ... 问题十：原理是什么意思？ 原理是名词，有两方面的意思：1.带有普遍性的、最基本的、可以作为其他规律的基础的规律；2.具有普遍意义的道理。

有时候我们临时需要在道客巴巴下载一个文档，但是道客巴巴的文档下载是需要注册账号的，并且很多文档需要自己上传文档赚取积分以后，用积分进行文档的下载。电脑：华为MateBook系统：Windows10软件：道客巴巴1.01、首先我们准备好需要的工具，可以百度搜索下载到自己电脑，然后运行程序。2、打开自己要下载的文档，将地址栏中的文档地址复制，如图所示操作即可。3、将复制的文档地址粘贴进来，然后点击右边的下载按钮，如图所示。4、出现如图所示的提示信息以后，然后我们再次点击这里的下载按钮。5、选择文档，然后点击菜单里面的设置按钮，如图所示进行操作。6、然后我们复制这里的输出路径，如图，下载的文件就在这个路径中保存。7、打开电脑计算机窗口，粘贴这个路径，可以看到我们下载的文档内容。

Acronym和Initialism都表示缩写的意思，在一定程度上，Acronym可以包含Initialim。但有些时候二者有着很大的区别。Acronym表示的缩写一般是不同的单词的首字母组成了一个新的单词。比如“RAM（RandomAccessMemory);LASER(LightAmplificationbyStimulatedEmissionofRadiation);NASA(NationalAeronauticsandSpaceAdministration),andOPEC(OrganizationofPetroleumExportingCountries).”而Initialism则仅仅是首字母的组合，并不代表着一个新的单词。比如“FBI(FederalBureauofInvestigation),CIA(CentralIntelligenceAgency),andDVD(DigitalVideoDisk*).”

开机进入主页面，一般第二页会有一个深灰色的齿轮图标，中文的设置英文的就是Setting了！setting就是设置的意思，就是那个齿轮的图标。

please get up on time !还要日语吗

1、Storage of FFPE Specimens 1.1、切片保存温度对提取的RNA质量有很大影响 1.2、化学修饰或RNA片段化影响生成的cDNA长度 1.3、切片暴露在空气或光下会影响提取的RNA质量，蜡块保存则不会 1.4、逆转录引物的选择影响RT-PCR的检测结果 2、Influence of Fixation Time 2.1、固定时间长不直接导致RNA片段化，但RT-PCR产物的长度受影响 2.2、固定时间长可能致蜡块保存时RNA片段化速度更快 3、Influence of Specimen Size 3.1、固定时组织厚，包埋后提取的RNA质量差，效果显著 4、RNA from Pathology Samples 4.1、真实手术病理FFPE样本的RNA质量比预期差，可能与样本自溶解相关 4.2、随机引物和oligo-dT混合引物相比单独的oligo-dT引物反转后的目标cDNA更丰度，用另外的反转录酶的结果也类似 To assess the effects of storage time and conditions on the quality of RNA from FFPE samples, freshly prepared paraffin blocks containing formalin-fixed specimens from different rat tissues (liver, kidney, heart, brain, lung and spleen) were stored at different temperatures (room temperature (20–25℃), 4℃ and 37℃). Fig. 1 shows that storage at different temperatures has a profound influence on the extent of RNA fragmentation. RNA isolated from FFPE specimens within 1–3 days after embedding was surprisingly intact, with RNA Integrity Numbers (RIN [8]) around 7 or higher. Even after 1 year storage at 4℃, ribosomal RNA bands were still clearly visible in most RNA eluates, and RIN values around 5–6 could be obtained. In contrast, RNA from blocks stored at room temperature (20–25℃) or 37℃, did not show clearly distinct rRNA bands anymore, and the mean fragment length was well below 2000 and 100 nucleotides, respectively. Samples derived from different organs did not show any significant differences in the extent of fragmentation over time. To determine in how far storage time and conditions affect usefulness of RNA isolated from FFPE material, one-step RT-PCR amplification was performed with primer sets directed against the rat HPRT gene, producing amplicons of different lengths from about 100 to 1100 nucleotides. Notably, even with RNA isolated from FFPE sections within three days after embedding, amplification of fragments up to 700 nt was successful (Fig. 2), whereas longer amplicons (1100 nt) could not be obtained. All amplicons were produced with similar efficiency using RNA isolated from RNAlaterstabilized tissue (not shown). Since BioAnalyser data (Fig. 1) clearly show that RNA up to 4.8 kb in length (size of the 23S rRNA band) can easily be isolated from these FFPE samples, and assuming that the same is true for mRNA, the limiting factor in this case is not RNA length per se. Instead, cDNA synthesis from the isolated RNA is apparently limited by the extent of chemical modification of RNA by formaldehyde during tissue fixation. The RNA purification method used in this study includes a heating step specifically designed to reverse formaldehyde crosslinking, which improves CT values in realtime RT-PCR by as much as 5–6 cycles compared to samples isolated without this step (data not shown). Exposure of individual FFPE sections to light and air had a negative influence on RNA quality, but storage of entire FFPE blocks either in the open or protected from air and light did not influence the effect of storage time on RNA quality, as long as the uppermost section was discarded at each time point, and sections not directly exposed to air were used for RNA preparation (data not shown). In real-time PCR applications, amplicons are usually shorter than 200 nt. However, in cases where oligo-dT priming is used for cDNA synthesis, the distance between the 3" end of the mRNA and the 5" end of the PCR forward primer becomes the limiting factor, because shorter cDNA products may not contain the entire region to be amplified. Consequently, RT-PCR amplicons should be within ,500 nt or less from the mRNA 3" end when oligo-dT priming is used for cDNA synthesis. This is not a concern when random or gene-specific primers are used for cDNA synthesis. Similar considerations regarding cDNA length and distance from the 3" prime end apply to other workflows that rely on cDNA synthesis, such as microarray hybridizations, in cases where oligo-dT priming is used. Another critical factor for nucleic acid quality from FFPE samples are the conditions used for formalin fixation. It is crucial to keep the time between sample acquisition and fixation as short as possible in order to avoid tissue autolysis and nucleic acid degradation by endogenous nucleases. In addition, overfixation can become an issue, particularly if fixation proceeds for considerably longer than 24 hours, resulting in more irreversible crosslinks [3–5]. There are also reports in the literature that fixation by formaldehyde, particularly over extended time periods, results in increased RNA degradation [9]. To assess the influence of overfixation on RNA quality, we compared tissue samples fixed either overnight or for 72 hours in formalin, compared to RNAlater-stabilised tissue samples from the same animal. BioAnalyser data show that even after 72 h fixation in formalin, the RNA is only marginally fragmented, and ribosomal RNA bands are still largely intact (Fig. 3), thus clearly showing that the reaction with formaldehyde itself does not cause nucleic acid fragmentation. In contrast, RT-PCR results demonstrate that prolonged fixation aggravates the negative effects of formaldehyde modification on cDNA synthesis. Whereas amplicons up to 800 nt can be routinely amplified from samples fixed over night, the maximum amplicon length for samples fixed for 72 hours in our PCR system was around 400–600 nt in most cases (Fig. 3). While overfixation did not directly result in increased RNA fragmentation, our preliminary data indicate that overfixation may result in faster RNA fragmentation during storage of FFPE blocks (data not shown). With the exception of small biopsies, penetration of the tissue by the fixative is the rate-limiting step in fixation, with initial penetration rates for formalin around 1 mm per hour. Penetration rates decrease with depth. Thus, around 8 hours are required for penetration of 5 mm tissue [10]. Therefore, it is generally recommended to keep sample thickness around or below 5 mm for efficient and even fixation, in order to avoid the risk of overfixation at the periphery, and tissue autolysis near the center of the specimen [7,11]. We examined the effect of specimen size and thickness by comparing rat kidney and brain samples that were either fixed whole (about 1 cm thick), or cut into smaller pieces, around 3–4 mm thick (Fig. 4). Analysis of RNA purified from these FFPE samples within days after embedding showed strong RNA fragmentation when entire organs where fixed, compared to relatively intact RNA obtained from thinner pieces. The observed fragmentation also resulted in shorter maximum amplicon size in our RT-PCR system. In the electropherograms of RNA from the large specimens, a small amount of longer RNA is still visible (Fig. 4, arrows). Presumably the respective RNA originates from the outer parts of the specimens which were penetrated by the fixative more quickly. The larger amount of these longer RNA molecules in the brain sample is also reflected in longer maximal amplicon size of 600 nt, compared to 400 nt in kidney (Fig. 4). The degradation effect observed here is surprisingly strong, considering that the thickness of the intact rat organs used was around 1 cm only. It is possible that the intact organs are more slowly penetrated compared to equally sized pieces cut from larger tissues. To compare our results from FFPE specimens obtained from animal tissues under strictly controlled conditions with real-life pathology samples, FFPE specimens from surgically removed lung carcinoma were obtained (see Methods). The FFPE samples had been stored for 1, 2, 4, 7, and 10 years at ambient temperature prior to sectioning. RNA preparation was done using the same purification protocol as for the rat samples above. RNA yields from a single 10 mm section were between 2.5 and 16 mg total RNA, with no correlation between age of sample and RNA yield. RNA integrity was checked by capillary electrophoresis (Fig.5a). According to the electropherograms, mean size of RNA fragments isolated was between 220 and less than 100 nt, also with no correlation between age of sample and mean fragment size. Even for the 1 year-old specimens, RNA integrity was significantly inferior to the rat samples after 1 year storage at 37℃ (Fig. 5a, and Fig. 1). The reason for this is most likely tissue autolysis, because thickness of the cancer specimens used for fixation was generally more than 2 cm, which implies more than 24 hours for penetration of the sample by the formalin [10]. To determine the usefulness of such samples for molecular analysis, real-time RT-PCR was performed on the RNA samples. Different primer pairs directed against the human housekeeping gene TBP mRNA were used to amplify fragments at different distances from the 3" end (128, 227, and 428 nt). For cDNA synthesis, the QuantiTect RT kit (QIAGEN) was used, either with a primer mix composed of random and oligo-dT primers (as contained in the kit), or, for comparison, with oligo-dT primer. Using the primer mix, all of the 1- and 2-year old samples and one of the 4-year samples produced threshold cycle (CT) values of 29–35 (Fig. 5b), whereas the remaining samples gave higher CT values, and in some cases failed to produce any meaningful signals within 40 PCR cycles. Although no clear correlation between RNA fragmentation and CT value was observed, the most fragmented RNA sample (4yr –b, lane 6 in Fig. 5a) gave the poorest PCR results. In contrast, using oligo-dT priming for cDNA synthesis resulted in roughly comparable CT values for the amplicon located at 127 nt from the 3" end, but significantly higher values for the more distant amplicons (Fig. 5b). Only 2 samples produced meaningful results consistently for the amplicon located 428 nt from the 39 end. Using a different reverse transcriptase for cDNA synthesis produced similar results (data not shown).

应该是设备设置的意思

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