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a new day has come的中文翻译是?

新的一天要来了

A new day has come歌词的翻译谢谢了,大神帮忙啊

a new day...新的一天到来了 a new day...新的一天到来了 i was waiting for so long 我等待了这么长时间 for a miracle to come 期待奇迹的出现 everyone told me to be strong 每人都告诉我要坚强 hold on and don"t shed a tear 坚持住不让泪水流下来 through the darkness and good times 穿越那些暗淡和美好的时光 i knew i"d make it through 我知道我一定会安然无恙 and the world thought i had it all 世人以为我拥有了一切 but i was waiting for you 但是,我正在等待着你 hush, love 安静爱 i see a light in the sky 我看见一道光芒划过天际 oh, it"s almost blinding me 如此耀眼几乎使我眩晕 i can"t believe 我不敢相信 i"ve been touched by an angel with love 天使已被我的爱感动而赐予我爱情 let the rain come down and wash away my tears 就让这大雨落下洗清我的泪水 let it fill my soul and drown my fears 让这雨点贮满灵魂赶走我的恐惧 let it shatter the walls for a new, new sun 就让这大雨冲跨心墙等待新的曙光 a new day has... come 新的一天到来了 where it was dark now there"s light 曾经的黑暗以被现在的光明代替 where there was pain now there"s joy 曾经的痛苦以被快乐赶走 where there was weakness, i found my strength 自信十足,不再脆弱 all in the eyes of a boy 我看到男孩的纯真眼神 hush, love 安静爱 i see a light in the sky 我看见一道光芒划过天际 oh, it"s almost blinding me 如此耀眼几乎使我眩晕 i can"t believe 我不敢相信 i"ve been touched by an angel with love 天使已被我的爱感动而赐予我爱情 let the rain come down and wash away my tears 就让这大雨落下洗清我的泪水 let it fill my soul and drown my fears 让这雨点贮满灵魂赶走我的恐惧 let it shatter the walls for a new, new sun 就让这大雨冲跨心墙等待新的曙光 a new day has...新的一天到来了 let the rain come down and wash away my tears 就让这大雨落下洗清我的泪水 let it fill my soul and drown my fears 让这雨点贮满灵魂赶走我的恐惧 let it shatter the walls for a new, new sun 就让这大雨冲跨心墙等待新的曙光 a new day has... come 新的一天到来了 ohhh, a light 哦光明 hush, now 安静爱 i see a light in your eyes 我看见光明在你的眼中 all in the eyes of the boy 我看到男孩的纯真眼神 i can"t believe 我不敢相信 i"ve been touched by an angel with love 天使已被我的爱感动而赐予我爱情 i can"t believe 我不敢相信 i"ve been touched by an angel with love 天使已被我的爱感动而赐予我爱情 hush, now 安静爱 hush, now 安静爱

a new day comes,be happy every day.翻译

A new day comes,be happy every day.新的一天来到了,开心快乐每一天。

a new day has come.是什么意思

a new day has come.新的一天来临了。双语对照例句:1.A new day has come! 新的一天已到来!2.The rising sun suggests a brand new day has come. 东升的太阳表明崭新的一天已经来临。

A new day has come中文歌词

a new day has come 中英文歌词对照: I was waiting for so long 漫长的等待 for miracle to come 期待奇迹的降临 everyone told me to be strong 大家都告诉我要坚强 hold on and don"t shed a tear 坚定意志 不要落泪 throught the darkness and good times 走过漫漫长夜 忆起美好时光 i knew i"d make it through 我知道我可以克服它 and the world thought i"d had it all 世人都认为我已经拥有了一切 but i was waiting for you 但是我却在等着你 hush now,i see a light in the sky 嘘...看 一道光芒划过天际 oh,it"s almost blinding me 天哪 它几乎让我目眩 i can"t believe i have been touched by an angel with love 真让人难以相信,爱的天使竟然触碰了我的心灵 let rain come down and wash away my tears 雨啊 尽情的下吧 洗去我的泪水 let it fill my soul and drown my fears 雨啊 尽情的下吧 浸没我的心灵,淹没我的恐惧 let it shatter the walls for a new sun 雨啊 尽情的下吧 粉碎我禁锢的心墙 迎来新的曙光 a new day has come 因为真爱即将来临 where it was dark ,now there is light 点亮光明,黑暗不复存在 where there was pain,nowe there is joy 满心喜悦,痛苦烟消云散 where there was weakness,i found my strengh 寻回坚强,懦弱一挥而去

a new day has come是什么意思

新的一天已经到来

魔兽世界主题曲A new day has come 是那位女明星专门设计给暴雪的嘛

不是的,仅仅是暴雪找出来当做歌曲而已A new day has come 1999年12月31日在蒙特卡洛最后一场演出,Celine Dion暂时告别了乐坛。,因为实现自己人生的一个目标也是一个梦想,生一个可爱的小宝宝。两年过去了,2002年,Celine带着一个可爱的小宝宝“雷奈查尔斯”和她的新专辑《A New Day Has Come》向世界乐坛宣布回归“我生命中新的一天已经来临”,这个新的一天,就是说她自己变成了一位母亲的角色。与暴雪无关

求迈克学摇滚的Ccomplicated Heart 歌词中文翻译

MLTR Complicated Heart Don"t know what to say now 现在不知道说什么 don"t know where to start不知道从哪里开始说 I don"t know how to handle我不知道如何控制 a complicated heart这复杂的心情 You tell me you are leaving你告诉我你要走 but I just have to say但我只能说 before you throw it all away 在你丢掉所有之前 Chorus: Even if you want to go alone即使你想单独上路 I will be waiting when you"re coming home我会在家里一直等你 if you need someone to ease the pain如果你想要不得别的人来安慰你 you can lean on me, my love will still remain你可倚靠我,我会永远爱你 Don"t know what you"re thinking不知道你在想什么 to me it seems quite tough对我而言太笼罩统 to hold a conversation继续对话 when words are not enough当言语不够时 so this is your decision因此这是你的决定 and there"s nothing I can do我不能做什么 I can only say to you我只能对你说 Chorus: Even if you want to go alone即使你想单独上路 I will be waiting when you"re coming home我会在家里一直等你 if you need someone to ease the pain如果你想要不得别的人来安慰你 you can lean on me, my love will still remain你可倚靠我,我会永远爱你

谁能翻译下MLTR的complicated heart的歌词~?

MLTRComplicated HeartDon"t know what to say now 现在不知道说什么don"t know where to start不知道从哪里开始说I don"t know how to handle我不知道如何控制a complicated heart这复杂的心情You tell me you are leaving你告诉我你要走but I just have to say但我只能说before you throw it all away 在你丢掉所有之前Chorus:Even if you want to go alone即使你想单独上路I will be waiting when you"re coming home我会在家里一直等你if you need someone to ease the pain如果你想要不得别的人来安慰你you can lean on me, my love will still remain你可倚靠我,我会永远爱你Don"t know what you"re thinking不知道你在想什么to me it seems quite tough对我而言太笼罩统to hold a conversation继续对话when words are not enough当言语不够时so this is your decision因此这是你的决定and there"s nothing I can do我不能做什么I can only say to you我只能对你说 Chorus:Even if you want to go alone即使你想单独上路I will be waiting when you"re coming home我会在家里一直等你if you need someone to ease the pain如果你想要不得别的人来安慰你you can lean on me, my love will still remain你可倚靠我,我会永远爱你

求Tara的 Day by day 空间可用链接发179346256@qq.com

wuayz309240

求beach boys的cocomo歌词中文翻译

阿鲁巴,牙买加ooo我想你 bermuda, bahama come on pretty mama百慕大,巴哈马来的漂亮妈妈 key largo, montego baby why don"t we go基拉戈,蒙特哥婴儿为什么我们还没有去 jamaica牙买加 off the florida keys从佛罗里达群岛 there"s a place called kokomo有一个地方叫做科科摩 that"s where you wanna go to get away from it all这就是你想要去摆脱这一切 bodies in the sand机构在沙 tropical drink melting in your hand热带饮料融化在你的手 we"ll be falling in love我们将堕入爱河 to the rhythm of a steel drum band的节奏钢鼓乐队 down in kokomo在科科莫 aruba, jamaica ooo i wanna take you阿鲁巴,牙买加ooo我想你 to bermuda, bahama come on pretty mama百慕大,巴哈马来的漂亮妈妈 key largo, montego baby why don"t we go基拉戈,蒙特哥婴儿为什么我们还没有去 ooo i wanna take you down to kokomo ooo我想带你到科科摩 we"ll get there fast我们将有快速 and then we"ll take it slow然后我们会把它缓慢 that"s where we wanna go这就是我们想要去的 way down to kokomo方式,以科科摩 to martinique, that monserrat mystique到马提尼克岛,这monserrat奥秘 we"ll put out to sea我们会从下海 and we"ll perfect our chemistry我们将完善我们的化学 by and by we"ll defy a little bit of gravity通过和我们会藐视一点点的重心 afternoon delight下午喜悦 cocktails and moonlit nights鸡尾酒和月光夜 that dreamy look in your eye这梦幻般的期待你的眼睛 give me a tropical contact high给我一个热带联系高 way down in kokomo方式在科科莫 aruba, jamaica ooo i wanna take you阿鲁巴,牙买加ooo我想你 to bermuda, bahama come on pretty mama百慕大,巴哈马来的漂亮妈妈 key largo, montego baby why don"t we go基拉戈,蒙特哥婴儿为什么我们还没有去 ooo i wanna take you down to kokomo ooo我想带你到科科摩 we"ll get there fast我们将有快速 and then we"ll take it slow然后我们会把它缓慢 that"s where we wanna go这就是我们想要去的 way down to kokomo方式,以科科摩 port au prince i wanna catch a glimpse太子港我想瞥见 everybody knows大家都知道 a little place like kokomo有点像科科摩 now if you wanna go现在如果你想要去 and get away from it all并摆脱一切烦恼 go down to kokomo下降到科科摩 aruba, jamaica ooo i wanna take you阿鲁巴,牙买加ooo我想你 to bermuda, bahama come on pretty mama百慕大,巴哈马来的漂亮妈妈 key largo, montego baby why don"t we go基拉戈,蒙特哥婴儿为什么我们还没有去 ooo i wanna take you down to kokomo ooo我想带你到科科摩 we"ll get there fast我们将有快速 and then we"ll take it slow然后我们会把它缓慢 that"s where we wanna go这就是我们想要去的 way down to kokomo方式,以科科摩 aruba, jamaica ooo i wanna take you阿鲁巴,牙买加ooo我想你 to bermuda, bahama come on pretty mama百慕大,巴哈马来的漂亮妈妈 key largo, montego baby why don"t we gohttp: lrc.bzmtv.com基拉戈,蒙特哥婴儿我们为什么不gohttp : lrc.bzmtv.com 提问者: 栗仁周 - 试用期 一级

Everybody knows that words can carry messages. People communicate with words. Books, magazines,...

小题1:B小题2:A小题3:D小题4:D 小题1:B 最后一段说道People can communicate in many ways without words说明人可以不用文字交流,所以B项错误,故选择B小题2:A A是身体语言,BCD都包含在身体语言里,故选择A小题3:D 文章第三段提到指路的标志Signs with arrows on doors tell you where to go in or out,一般是箭头,故选择D小题4:D 同样在文章最后一段有提及答案an artist can use his drawings to tell about beautiful mountains,说明画家交流的方式是绘画,故选择D

normal,general,ordinary,common的区别有哪些不同的用法

Normal common ordinary usual的区别 normal ordinary 都含“正常的”、“正规的”的意思.normal指“正常的”、“正规的”、“常态的”,如:the normal temperature of the human body 人的正常体温.ordinary指“平常的”、“普通的”强调“平常的”、“平淡无奇的”,如:an ordinary day"s work 日常工作.His ordinary supper consists of only bread and milk.他通常的晚餐不过是面包和牛奶.common强调“常见的”、“ 不足为奇的”,如:Colds are common in winter.感冒在冬天很常见.usual:(1) adj.平常的;通常的,惯常的 = normal,happening often [例句] Twelve o"clock is the usual time to have lunch.十二点通常是吃饭的时间.It"s usual to have a holiday in the summer.夏天休假是惯例.It can"t move about in the usual way.它不能象平常那样到处活动了.as usual:as always 跟往常一样,象平常一样 Tony arrived late,as usual!托尼还是同往常一样迟到了!辨析common 、ordinary、normal、usual、frequent以及general:1) common “普通的”,“平常的”,“共有的”,指从质量或品德上无特殊,显眼或杰出之处,因而是大多数人或事物所具有的,常见的.It"s an error quite common among schools.This flower is common in spring.2) ordinary 指符合一般或常规事物标准或相同的规格,因而只具有普通的特性.例如:Although he is rich,he is always in ordinary dress.common与ordinary基本同义,强调“平凡的,普通的”,表示随时可以碰到,不值得惊奇.如:an ordinary ( a common) event 常有的事情 an ordinary (a common0 person 一个普通的人 3) normal 表示“正常的,常态的,正规的”,相对于非正常而言.例如:Do you know the normal temperature of the human body.His growth is normal for that age.4) usual 通常与人的行为习惯有关,含义为“惯常的,通例的”.例如:Tea is considered to be the usual drink of British people.He arrived later than usual.5) frequent 经常发生的;频繁的(事).例如:Frequent failures did not affect his morale.屡次失败都没有使他泄气.Rains are frequent here in early summer.这儿在初夏季节常下雨.在表示人的名词前用frequent表示:常见的.例如:a frequent caller [visitor] 常客 6) general 意为“普遍的”、“一般的”,与高级想对应.例如:This book is intended for the general reader,not for the specialist.这本书是为一般读者写的,不是为专家写的.

http://v.ku6.com/show/gg9XxxY07Vc4O3of.html 背景音乐是什么?

迈克尔杰克逊的《beat it》

WWW.DJ97.COM里面背景音乐,最后那首"凤舞九天经典现场"

其实 Give Me More 有很多版本 像 Gimme More 2002、 Gimme More等都是Fool Groovers的歌你要的那个版本是remix过的先听听它的原版 Gimme more 2002http://music18.163888.net/fb159b11523/2007/06/03/09/Music/61702112615.mp3还有你要的http://www.xzwma.com/Download2.asp?SV=2&id=42624去狗狗找找 或者酷狗上 很多的

谁知道这首舞曲串烧中的三首歌的名字和URL http://swf.vip2.flashyc.com/Upload/2008-4-9/173555193.mp3

第一首 :Brothers 的 Dance Now Come on lady show me baby things I have to know, don"t you know I will blow if you let me go, are you ready? if you"re ready show me what to do, I want you, you me too, we will make it true. Come on baby, hit now baby time for us to go, let me in don"t missing we will start to flow, can you feel it like I feel it do it up and down, wait a sec what I feel now there is no throughout Come on let"s dance now, moving on the floor, let"s have the power, stand in , you"ll hit the door bringin all down on us would you dance some more stays like all world let"s flow onto our shore 第二首 Gimme More - Fool Groovers *I want to love on a sunshine summer I love song that"s kicky like a hammer I won"t miss if I have to love you Or let music take my minds so far away* Repeat* Now,So fine in a sunshine sunny Leading a feeling so strong Love takes come from *Give me more,give me more Music is so strong Give me more,give me more In the sun that it flows Give me more,give me more You will see what I mean Give me more,give me more Music deaf control* Repeat* 第三首 P Lion - Happy Children Always the war In nineteen eighty three This is the world of today In our mind There is only the money And there is nothing for you Then hours work No flowers in the mind The life runs without happiness Everyday dreams To go to a better life But they remain only dreams You are the children Your life will be very hard You are the children You"re singing every day To have a friend To explain your little problem Can you say now to have it Sometimes you hope It"s only an illusion Don"t hope, it"s better so But children power Will win, I am sure This is sweet lovers for us Your fantasy Will find new nice colours But now it"s time to go You are the children Your life will be very hard You are the children You"re singing every day You are the children Your life will be very hard You are the children You"re singing every day You are the children Your life will be very hard You are the children You"re singing every day

combinemypastlanguagelearningexperiens翻译

combine my past language learning experiens结合我过去的语言学习经验

所有关于give,take,break,go,stand,find,make,look,come,turn,set,move,cut,put的短语及意思如give out分

Take有关的短语有关turn的常用短语:turn down 关小,调低 turn in 交出,上交turn off 关 turn on 开turn out 结果是 turn over仔细考虑;翻转turn to 转向,求助于 turn up 调大;出面,出席关于take的常用短语:take after(外貌)相像 take apart 拆卸,拆开take away 减去take down 写下,记下take for 把...误认为take in欺骗;吸收,吸纳take off 起飞;匆匆离开;脱下take on 承担;呈现;开始雇佣take over 接收,接管take to 喜欢,开始从事take pictures照相 take care of 照顾,照料take up 开始从事,着手处理take it easy 别紧张be taken aback (1) 大吃一惊, 惊得目瞪口呆, 吓了一跳 (2) (船)突然遇到逆风 be taken back (1) 大吃一惊, 惊得目瞪口呆, 吓了一跳 (2) (船)突然遇到逆风 be taken with (1) 对...很喜爱; 被...迷住, 被...吸引 be taken by (1) 对...很喜爱; 被...迷住, 被...吸引 double take (1) 先是不注意接着大吃一惊 (2) 先是怔着接着恍然大悟的反应 not taking any (1) [口]谢绝, 不接受, 没有那种兴趣 去做 on the take (1) [美俚]接受贿赂; 敲诈, 伺机损人利已 take after (1) 象 (2) 学...的榜样, 仿效 (3) 追赶, 追捕 take against (1) 反对; 不喜欢 take amiss (1) 因...而见怪; 误会, 误解 take apart (1) 使分开, 拆开 (2) 严厉批评; 猛烈抨击 (3) 严格剖析 (4) 粗暴对待; 严厉惩罚; 彻底打败 take as read (1) 认为不必宣读(会议记录等); 认为不必谈论或听取(某事) (2) 宣称对...满意; 声称对...没有意见 take away (1) 拿走; 夺走; 拆去 (2) 使离开; 带走 (3) 使消失, 消除(病痛等) (4) 减去 (5) 把...买回家食用 take back (1) 收回(前言), 承认说错了话; 取消(诺言) (2) 同意退回, 收回(商品); 接回, 带回 (3) 使回到某一起点; 使回想起 take down (1) 拿下, 取下 (2) 记[录]下来 (3) 挫其气焰 (4) 拆掉 (5) 吞下, 咽下 (6) 病倒 (7) [口]欺骗(某人) take five (1) [美口]休息五分钟[十分钟], 小憩 take ten (1) [美口]休息五分钟[十分钟], 小憩 take ... for ... (1) 以为(某人或某物)是... take from (1) 减去; 减损; 降低 take home to oneself (1) 充分理解, 深刻领会 take in (1) 收进, 接受; 装入, 收容, 接待 (2) 领(活)到家里做 (3) 缩短; 改小; 收(帆), 卷(帆) (4) 领会, 理解 (5) 观看, 参观, 游览 (6) [口]轻信, 信以为真 (7) [口]欺骗 (8) 订阅(报刊) (9) 包括[含] (10) 拘留 take it (1) 猜想, 以为; 断定 (2) [口][常与 can, be able to 连用]忍受得住(痛苦、批评、困难, 嘲笑等) (3) 默然同意, 接受 take it all in (1) 注意倾听; 全部听进 take it and like it (1) [美俚]不太甘心 地 忍受批评(嘲笑等) take it or leave it (1) 要么接受, 要么拉倒; 要不要 随你的便 take it out in (1) 接受...作为抵偿 take it out of sb. (1) 使 某人虚弱, 使某人精疲力竭 (2) (=takeit out on sb.)找某人出气[泄愤] take me not up before I fall (1) [苏口]在你回答我以前, 先听我说完 take off (1) 取[脱]下; 拆下; 切除 (2) (=take oneself off)[口]走掉; 滚开 (3) 带往(某处); 移送(某处) (4) 减(价) (5) 取消, 夺去...的生命, 杀死 (6) [口]摹仿, 学...的榜样; 取笑 (7) (飞机)起飞 (8) 移开(目光, 注意力) (9) 停止演出 (10) 去掉, 扣除 (11) 复制; 绘制 take off after (=take after) (1) 追赶, 追捕 take on (1) 具有,呈现(某种性质、特征等) (2) 担任(工作), 承担(责任) (3) 雇用 (4) 接受...的挑战; 同...较量 (5) [口]伤心; 激动 (6) 装出某种样子 (7) [口]流行, 风行; 受欢迎 (8) 接受(乘客); 装载 take out (1) 取出, 拔 出; 除掉(污迹等), 擦去 (2) 邀(某人)出门, 带去 (3) 取得, 领 到(专利权, 执照等) (4) 【律】发出(传票), 传讯 (5) 摧毁, 消除 (6) 把...买出食用 (7) 启程, 出发 take out of (1) 取出, 除去; 要赔偿; 报仇 take out after (1) [take after]追赶, 追 捕 take over (1) 接收[管, 任] (2) 把...载送到 (3) 仿效, 采用 (4) 把...移入下一行 take sb. unawares (1) 使 某人出其不意, 攻其不备 take sb. up on (1) 接受某人的(邀请, 挑战等) (2) 要求某人兑现... take sb. short (1) 突然袭击某人; 使 某人大吃一惊 (2) 唐突地打断某人的话 take sb. up short (1) 突然袭击某人; 使 某人大吃一惊 (2) 唐突地打断某人的话 take sb. wrong (1) 误解[曲解]某人的意思 take sth. up with sb. (1) (口头或书面)向某人反映某事; 就某事向某人提意见 take to (1) 爱, 喜欢 (2) 嗜好, 沉迷于, 养成...的习惯 (3) 逃到..., 借助...逃 走 (4) 开始从事 take too much (1) [口]喝过多(酒), 喝 醉 take up (1) 举[拿, 捡, 拔]起 (2) 占(地方); 费(时间); 占据 (3) 接纳(乘客); (船)承装(货物) (4) 吸收(水 分); 溶解 (5) 打断某人的话; 责备, 申斥 (6) 开始; 着手处理 (7) 对...发生兴趣; 开始从事, 开始学 (8) 把(某人)置于自己的庇护之下; 收于(门下), 提携 (9) 继续, 接下去 说 (10) 逮捕 (11) 扎紧, 绕紧; 改短(衣服) (12) 接受(建议, 挑战等) (13) (学校)开学; 上课 (14) 定居, 安家 (15) 付清, 认购(公债); 募(捐) (16) [口](天气)变晴, 变好 take up with (1) 和...交往[鬼混] (2) 一心一意想, 醉心于 (3) 信以为真; 采用, 接受 (4) [常用被动态]对...很喜爱 (5) 甘受, 忍 受 take upon oneself (1) 以...为己任; 主动承担(责任等); 把(过错)归咎自己 (2) 擅自; 毅然, 大胆 take on oneself (1) 以...为己任; 主动承担(责任等); 把(过错)归咎自己 (2) 擅自; 毅然, 大胆 take us as you find us (1) 我们就是 这样, 请你将就些吧 (1) take 系常用词, 指“拿、握、取", 如: 握住某人的手。 (2) seize 指“突然用力地抓住、握住", 如: 警察捉住一个嫌疑犯。 (3) grasp指“紧紧地抓住", 用于比喻意义时指“掌握",“领会", 如: 握紧绳子。 (4) clutch 指“突然抓住", 常伴有“急切或害怕的心情", 如: 将要溺死的人连一根稻草也要去抓。 (5) grab指“抢夺"、“攫取", 如: 那孩子抢了那个苹果就跑了。 (6) snatch 指“突然快速地拿、取、夺、抢走", 如: 老鹰叼了小鸡就飞走了。 1. take after (不用进行式) -to look or behave like (an older relative) 长相或举止像(某个长辈) 2. take apart (1) to separate (a small machine, clock, etc.) into pieces 把(小型机器、钟表等)拆开;拆散 (2) to defeat very severely in a sport or game (在体育运动或比赛中)把……彻底打败;speak angrily to or criticize severely 对……苛求;严厉批评 3. take as 看作,认为 4. take away 拿走;使停学,使离开;使消失;减去 △ take away from -to lessen the effect or value of (something good or desirable) 贬低(有益或令人满意之事物)的作用 5. take back (1) to admit that one was wrong in (what one said) 承认说错了(话),收回(诺言,话语) (2) to cause to remember or think about a past time 使回忆起 (3) 送回,还回去;退(货) 6. take down (1) 拿下,取下;记下来;褪下(裤子等穿于腰以下的衣物) (2) to separate (a large machine or article) into pieces esp. in order to repair it or move it (尤指为了修理或搬移而)把(大型机器或大物件)拆成零部件;把……拆卸开 7. take in (1) to receive into one"s home; provide lodgings for (a person) 接待(某人)留宿 (2) to deceive 欺瞒,欺骗 (3) to understand fully; grasp 充分理解,掌握 (4) to make (clothes) narrow 把(衣服)改窄 (5) to include 包含,包括 8. take off (1) to remove (esp. clothes) 脱下,脱去(尤指衣服); 解(拿)掉 --- put on (反义词) (2) (of a plane, etc) to rise into the air at the beginning of a flight (飞机等)升空,起飞 (3) to have a holiday from work on (the stated day) or for (the stated period) 在(某日或某段时间)休假;歇(……天)假; 请假 (4) to begin to become successful, popular, or well-known 开始有成就;开始受欢迎;开始成名 (5) 打折扣 (6) to copy the speech or manners of (someone), esp. for amusement(尤指为了逗笑而) 模仿 (某人)的谈吐、举止等 9. take on (1) to start to employ 开始雇用 (2) to begin to have (a quality of appearance) 开始具有/ 呈现(某种品质、面貌等); 露出 (3) to accept (work, responsibility, etc.) 接受(工作等);承担(责任等) (4) to start a quarrel or fight with 开始和……争吵(斗殴、作对、较量等) (5) 让上车,接受乘客 10. take out (1) to go somewhere with (a person) as a social activity 带(某人)去(某处参加社交活动等) (2) to obtain officially 正式取得,领得,获得 (3) 洗去(污迹),使褪色 △ take someone out of himself / herself 使(某人)从(失意、不愉快等)中解脱出来,给(某人)消愁 △ take sth out on sb 向……发泄(情绪);拿……出气 11. take over --to gain control over and responsibility for (something)接手,接任;接管 12. take to (1) to feel a liking for, esp. at once (尤指立刻)对……产生好感,喜欢上 (2) to begin as a practice, habit, etc. 染上……习惯(嗜好等) (3) to go to for rest, hiding, escape, etc. 到(某处)休息;到……躲藏;逃往 13. take up (1) to begin to spend time doing; interest oneself in 开始花时间从事(某项活动);对……产生兴趣; 开始学习(某课程),选修 (2) (of things or events) to fill or use (space or time) (事物或事件)占用了(时间或空间) (3) to accept the offer of 接受……的建议 (4) to continue 继续 △ take up with 和(某人,尤指讨厌的人)来往 be taken up with - be very busy with 忙于; 喜欢 14. be taken ill 病倒了,生病了 15. take a chance 碰碰运气,冒……风险 16. take a deep/ long breath 深吸一口气(以鼓起勇气或仔细考虑) 17. take a fancy / liking (dislike) to 喜欢(不喜欢) 18. take a hand in 干预 19. take a load / weight off sb"s mind 使放心/ 安心 20. take a risk / risks 冒风险 21. take a seat 坐下 22. take a vote 投票表决 23. take an interest in 对……有兴趣 24. take steps /measures to do 采取措施做某事 25. take action (on) (对……)采取措施 26. take advantage of 利用;占……的便宜, 不正当地利用 27. take aim 瞄准 28. take by 握住(某处); 拉住 29. take ….by surprise 奇袭;出其不意做某事, 使惊奇 30. take care 当心 31. take care of 照顾,料理;处理,对付;当心 32. take charge of 负责,接管 33. take cold 感冒,伤风 34. take control of 控制住,管住 35. take delight / pleasure in 以……为乐;喜欢 36. take effect 开始起作用;生效 37. take for / to be (错)当作,以为是 38. take ….for granted 想当然认为(会是某种情况),认为……是理所当然的;认为没有问题 39. take ….for instance / example 以……为例 40. take hold of 抓住;吸引住 41. take ….into account / consideration 考虑到,把……考虑进去 42. take it / things easy 慢慢地,不要过于紧张/ 劳累 43. take it or leave it 要不要都行;要么要要么不要,不要还价 44. take note (of) 注意 45. take notice (of) 注意;理会 46. take notes 作记录;记笔记 47. take / come into office 就职,上任 48. take one"s breath away 令人惊异,叹为观止 49. take one"s chance(s) 碰碰自己的运气 50. take one"s time 慢慢来,从容不迫 51. take pains 特别注意 / 努力,下工夫 52. take (the) trouble 费事,下工夫 53. take pare (in) 参加,参与 54. take pity on / upon 可怜,怜悯 55. take place 发生,举行 56. take pride in / be proud of 为……感到自豪/ 骄傲 57. take revenge on 向….报复 58. take one"s place 代替某人 59. take the long view 看得远一些 60. take the opportunity 利用这个机会 61. take turns 轮流(做某事)

turn与become的区别

become,get,go,grow,turn它们虽然都表示“(从一种状态)变成另一种状态”,但与其相关的搭配却有差别。若不注意,在使用时就会出错。 1.形容词在这类系动词后面作表语 go和come相比,“go+形容词”多表示“(从好的状态)变成坏的状态”, e.g.(1)In hot weather,meat goes bad. 热天,肉会变坏。 系动词go后面的表语为mad,crazy,blind,lame或表示颜色的形容词时,go前面的主语一般为人。 e.g.(1)He went mad last year. 去年他疯了。 (2)Hearing this,she went red. 听到这个,她脸红了。 2.系动词grow后面可以接表示人或物的特征的静态形容词作表语,也可以接表示天气的形容词作表语。它侧重于“逐渐变成某种状态”。 e.g.(1)The girl grew thinner and thinner. 这个女孩越来越瘦了。 (2)Soon the sky grew dark. 天很快变黑了。 3.系动词turn后面多接表示颜色的形容词作表语,也可以接表示天气的形容词作表语。意思上侧重于“变得与以前完全不同”。 e.g.The man turned blue with fear. 那个男人因害怕而脸发青。 The weather suddenly turned much colder. 天气突然变得冷多了。 4.“get+形容词”多用在口语中。get能替代become,但become较为正式。get与become前面的主语既可以是人也可以是物。 e.g.He became/got angry with his son. 他生他儿子的气。 His coat has become/got badly torn. 他的外套已经非常破了。 get经常与形容词的比较级连用。 e.g.The days are getting longer and longer after the winter solstice. 冬至过后,天变得越来越长。 注意:系动词become一般不可用来表示“将来变得……”的意思。 5.系动词become,turn,get,go,后面能跟名词作表语,其他的“变成”类系动词后面不能跟名词作表语。 e.g.His dream has become a reality. 他的梦想变成了现实。 He has turned scientist. 他成了科学家。 Her cheeks went a very pretty pink. 她的脸颊变得绯红。 注意:在turn后面作表语用的名词前通常不带冠词。 6..系动词get,go,后面可接现在分词,但含义不是“成为”。 e.g.They went in and got chatting together.(get意为“开始”) 他们进去后开始聊天。 We often go swimming.(go意为“去”) 我们常去游泳。 7.系动词get后面接不定式,表示变化过程。 Mary"s growing to be more and more like her mother. 玛丽越长越像她的母亲了。 注意:这种结构不能与表示一段时间的状语连用。

请帮我翻译下这歌‘homecoming’

Homecoming 回家 [Part 1: The death of St. Jimmy] 圣吉米之死 My heart is beating from me 我的心跳来源于我自身 I am standing all alone 我独自的站立 Please call me only if you are coming home 请仅在你要回家的时候通知我 Waste another year flies by 一整年的流逝是建立在 Waste a night or two 一日一日的流逝上的 You taught me how to live 你教我如何卑微地 In the streets of shame 在街头生存 Where you"ve lost your 哪里你已经失去 dreams in the rain 在雨中幻想 There"s no signs of hope 没有希望的曙光 The stems and seeds of the last of the dope 最后一个傻瓜的茎和种子(指St. Jimmy的依靠和希望) There"s a glow of light 有一束热光 The St. Jimmy is the spark in the night 圣吉米是黑暗中的火光 Bearing gifts and trust 承担着天赋和信任 The fixture in the city of lust 是这个欲望都市的支柱 What the hell"s your name? 你地狱的名字是什么? What"s your pleasure and what is your pain? 什么是你的快乐和你的痛苦? Do you dream to much? 你是否做太多梦? Do you think what you need is a crutch? 你是否认为你需要一把拐杖? In the crowd of pain. St. Jimmy comes without any shame 圣吉米不带任何耻辱地在痛苦中回来 He says we"re fucked up, But we"re not the same 他说我们都是混乱的,但是却各不相同 And mom and dad are the ones you can blame 父母是供你责备的 Jimmy died today 吉米今天死了 He blew his brains out into the bay 他在绝路上结束了自己的性命 In the state of mind it"s my own private suicide 是精神上的自杀 [Part 2: East 12th St.] 东方12圣 Well nobody cares 是的,没有人在意 Well nobody cares 是的,没有人关怀 Does anyone care if nobody cares? 如果没有人在意还有谁会在意? [x2] Jesus filling out paperwork now 耶稣正在东方12圣之上 At the facility on east 12th st. 用工具填写 He"s not listened to a word now 他没有听从于他人 He"s in his own world 他用他自己的方式 And he"s daydreaming 他有一个白日梦 He"d rather be doing something else now, 他宁愿现在做点儿别的什么 Like cigarettes and coffee with the underbelly, 像下体的香烟和咖啡(?) His life"s on the line with anxiety now, 他现在正生活在焦虑中 And she had enough, 她得到足够的 And he had plenty 他得到丰富的 Somebody get me out of here 某人带我离开那里 Anybody get me out of here 何人带我离开那里 Somebody get me out of here 某人带我离开那里 Get me the fuck right out of here 让我尽快摆脱那个鬼地方 So far away 如此遥远 I don"t want to stay 我不愿停驻 Get me out of here right now 现在就让我走 I just wanna be free 我只是想要自由 Is there a possibility? 这会有可能吗? Get me out of here right now 让我现在就离开那里 This life like aint for me 这种生活与我水火不容 [Part 3: Nobody likes you!] 没有人喜欢你! I fell asleep while watching spike TV 当我在喝完十杯咖啡以后 After 10 cups of coffee 看无着聊的电视节目我睡着了 And you"re still not here 你依旧不在那里 Dreaming of a song 梦见一支歌 But something went wrong 但是什么东西出了错 But I can"t tell anyone 但是我不能告诉任何人 "Cause no one"s here 因为没有人在这里 Left me here alone 让我独自一人在这里 And I should have stayed home 我本应该留在家里 After 10 cups of coffee I"m thinking 在喝完十杯咖啡以后我开始胡思乱想 (where"d you go?) 你将到何处去? Nobody likes you, everyone left you 没有人喜欢你,所有人离开你 (where"d you go?) 你将到何处去? They"re all out without you havin" fun 他们都离开你去享受快乐了 (where"d you go?) 你将到何处去? Everyone left you, nobody likes you 没有人喜欢你,所有人离开你 (where"d you go?) 你将到何处去? They"re all out without you havin" fun 他们都离开你去享受快乐了 (where"d you go..go..go..go..) 你将到何处去? Cheers...Ha..干杯……哈…… [Part 4: Rock and roll girlfriend] 摇滚女友 [written and sung by Tre Cool] (!!!这一段是Tre自己写的,并且亲自演唱地啊!) I got a rock and roll band 我组建了一个摇滚乐队 I got a rock and roll life 我得到了一种摇滚生活 I got a rock and roll girlfriend 我得到了一个摇滚女友 And another ex-wife 另外一个形势上的妻子 I got a rock and roll house 我得到一所摇滚房屋 I got a rock and roll car 我得到一辆摇滚汽车 I play the shit out the drums 我狂敲我的鼓 And I can play the guitar 我可以弹弹吉它 I got a kid in New York 我在纽约得到一个孩子 I got a kid in the bay 我在港湾(或绝路上)得到一个孩子 I haven"t drank or smoked nothing 在多于22天的日子里 In over 22 days 我喝了酒业抽了烟 So get off my case 我放弃了我的事业 Off of my case 放弃了我的事业 Off of my case! 放弃了我的事业 [Part 5: We"re coming home again] 我们又回家了 Here they come marching down the street 他们军队般整齐的走在街上 Like a desperation murmur of a heart beat 就像心跳绝望的低语 Coming back from the edge of town 从城镇的边缘归来 Underneath their feet 在他们脚下 The time has come and it going nowhere 时间流逝,无处可去 Nobody ever said that life was fair now 从来没有人说过生命是公平的 Go-carts and guns are treasures they will bear 婴儿车和手枪将是他们的财富 In the summer heat 在酷夏的烈日下 The world is spinning 世界静止了 Around and around 旋转啊旋转 Out of control again 再一次失控 From the 7-11 to the fear of breaking down 从7-11到灭亡的恐惧 To send my love a letterbomb 把我的爱用糖衣炸弹寄出去 And visit me in hell 到地狱与我相见 We"re the ones going 我们正在离去 Home 家 We"re coming home again 我们又回家了 Home 家 We"re coming home again 我们又回家了 I started fuckin" running 当我的脚一触到地面 As soon as my feet touched the ground 我就开始疯跑 We"re back in the Barrio 我们回到这个区域 But to you and me, that"s single town 但是你和我,这是一个单独的小镇 Home 家 We"re coming home again 我们又回家了 Home 家 We"re coming home again 我们又回家了 Home 家 We"re coming home again 我们又回家了 Home 家 We"re coming home again 我们又回家了 Home 家 We"re coming home again 我们又回家了 Home 家 We"re coming home again 我们又回家了 Home 家 We"re coming home again 我们又回家了 Home 家 We"re coming home again 我们又回家了 Home 家 We"re coming home again 我们又回家了 Nobody likes you 没有人喜欢你 Everyone left you 所有人离开你 They"re all out without you havin" fun 他们都离开你去享受快乐了

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模拟设定值 + com 链路的 uss 设定值是什么意思

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Fundamentals of Computer Graphics(虎书)是国内外公认的比较好的计算机图形学入门书籍,虎书现在已经出第五版了。奈何此书并没有中文版,为方便自己和广大图形学爱好者学习此书,本人将利用空余时间翻译此书。个人水平有限,翻译不当请指正。 计算机图形学基础 介绍 图形领域 主要应用 图形API 计算机图形学基础 数学杂项 计算机图形学基础 光栅图像 计算机图形学基础 光线追踪 计算机图形学基础 表面着色 计算机图形学基础 线性代数 计算机图形学基础 转换矩阵 计算机图形学基础 视图 计算机图形学基础 图形管线 计算机图形学基础 信号处理 计算机图形学基础 纹理映射 计算机图形学基础 图形数据结构 计算机图形学基础 采样 计算机图形学基础 基于物理的渲染 计算机图形学基础 曲线 计算机图形学基础 计算机动画 计算机图形学基础 使用图形硬件 计算机图形学基础 颜色 计算机图形学基础 视觉感知 计算机图形学基础 色调重现 计算机图形学基础 隐式建模 计算机图形学基础 游戏中的计算机图形学 计算机图形学基础 可视化

求妖魔的封印的drama的翻译,最好连特典也有。。。 邮箱:476203137@qq.com

  亲也喜欢妖魔的封印哇,呵呵,我是非常喜欢的……但让你失望了,木有翻译……挺你一下吧。这部作品非常老了,难得你挖出来听哈,drama与原作品有了一定的出入,不过还是非常赞的,令人怀念的盐泽大人……泪……  我写过碟评,姑且给亲吧,我自己写的:  守候了如此多年,又是这么钟爱的漫画,在听过Drama CD后确实有很多感触想写,而这篇碟评却酝酿了很久,可能是对越喜欢的东西就越慎重吧(笑)。今天终于贴出来,也算贻笑大方……各位看官给点意见哦!  1.最想说的是:这三张本编CD拼凑大牌声优的嫌疑未免有些大!  出场演员虽都是当时的一线声优,主角却是两位名不见经传的大人,真让偶粉无奈啊!刚拿到碟时,对绪芳惠美小姐精彩的开场白几乎完全没入耳而拼命用每个神经捕捉男声优的角色,真是对不住绪方小姐……(因为买盗版碟,卖家没有义务给偶cast,只能全凭自己的听觉神经,默~~),男声终于响起的时候,偶差点喋血!是速水奖大和置鲇龙太郎大啊!偶的眼眶热了起来,难道老天对偶如此恩慈,由如此重量级而华美的声音诠释偶心目中最崇高美丽的多利亚斯和席巴么??(因为漫画的顺序就是多利亚斯和席巴先出场的说。)偶是多么幸福的人啊~~~还在激动却发现对话满不是那么回事!残酷的对话内容告诉偶两位大人只是配角!  不但如此,所有配角随便提一位都很不得了!井上和彦大人的疫魔王——拉瑞尔;盐泽兼人大人的梦魔——西菲尔;关智一也有优秀表现的“强盗头子”——里隆多……在如此庞大的绿叶衬托下,对两位主角声优的压力大概比动力还要足吧?轻捏一把汗。  2.对主角两位大人的感悟!  记得上次看碟评,说到一句“席巴的声音不够华丽,在宫本充大人的诠释下变成了‘农民"。”至今记忆犹新,当时简直是笑倒在地!说实话挺同意的,但是后来一思考,却又对这样的角色选择挺理解了。席巴在妖魔使中是一个“怪人”,他淡薄名利,面对安逸舒适的生活却毅然选择漂泊,拥有绝美的外表和高超的魔术本领为人却谦和不骄傲,并乐于助人……偶觉得:只有如“农民”般的纯朴才能如此这般吧。(笑)  上次的碟评中也提到置鲇龙太郎大人由于声音的硬质与主角擦肩而过,关于这点偶也是认可的,但置鲇龙太郎的罗贤过多出场也算弥补了这个遗憾吧。而偶在心里却自私地觉得:多亏置鲇没有出演席巴!在偶的心目中他果然是更适合能持剑的《KIZUNA》(《绊》)中鲛岛兰丸的角色啊!偶是不能接受“白面之鬼”的兰丸一下子变为“柔弱无骨”的长发美人席巴的哦!原谅我的小小自私……让置鲇永远重叠在坚强美丽的兰丸身上吧!顺便提一下:多利亚斯给罗贤治眼睛那场很温馨的说。︿_︿  发现主角这两位声优都不是很高产,只是最近在《春を抱いていた》(《拥抱春天》)中再次听到了田中秀幸大人的声音,一下子觉得选择这二位大人作为主角演对手戏是很合适的。因为如果说席巴的宫本充是“农民”的话,多利亚斯的田中秀幸可以说是“平民王”!此言一出可能有西红柿和鸡蛋飞过来了,偶闪!  不过真的,偶直觉觉得田中大人为人一定非常谦和,因为他在出演岩城の兄时,对香藤洋二道歉的出演比对他责骂的表现还要中肯和到位!偶不禁在想:莫非大人本身就是个谦和的人吧。而在Free Talk中,田中大人一开口,三木当时来了一句:“(你)上了年纪啊!”,田中大人竟然很腼腆地应了一声,表示认可!(一般人都会反驳些什么吧,类似于“真失礼啊,我可是前辈呢!”,或是像小杉十郎太对于别人的讽刺至少会说句“他真过分啊!各位听众朋友……”,而田中大人却没有。)于是偶对田中大人谦和的印象怕是固化了!  3.对“盐大”必要性的质疑!  偶深知,这一质疑一提出,将会被“盐大”的粉丝彻底齐声反对,而淹没在喊叫声中~~~偶还是爬出来要说一句:偶还是觉得这个角色有没有无所谓(这么说是对盐大在天之灵的最大不敬吧?寒汗!),完全不影响故事情节,不过影响CD销量,看来日本声优界的商业化当时就已经很严重了……演变至今就害得偶还在猛跟一些endless的系列故事!(汗哪!偶突然想到森石的《ENDLESS》系列为什么不停地出而没有完结篇了!那故事本身就叫endless,还指望有完结篇么?!啊!因为写《妖魔封印》的碟评还有了新领悟,不错!)  但偶真切的认为:盐大出演梦魔还真是合适!他那声音总是有气无力睡不醒的风格(笑)。事实证明,他声音的独特有他独特的道理,人们都说他的声音黑暗,却原来他的黑暗是因为他最接近死神!要知道日本是全世界平均寿命最长的国家,女性是80岁,男性是78岁,盐大的英年早逝有多令人遗憾和震惊就可想而知了!一这么想就觉得别说是增加了梦魔的演出,就是为了盐大再造个什么本来没有的火魔王、土魔王也应该啊!呜呜~~~  还好,绪方小姐作为他的徒弟,偶感觉很得盐大的真传呢!水魔王也阴阴的!不幸中的万幸吧。  4.对非常喜欢角色被无情删去的遗憾!  主要想提里维这一角色——偶之最喜欢的妖魔之一!竟被毫无保留地如妖魔不知怎么出生的一样,不知怎么消失了……偶认为他是推动故事进程的重要角色啊!怎么会就狠狠地去掉了呢?!  偶多么感动里维为蒂亚抢夺被席巴丢弃的多利亚斯用头发做的项链护身符而受伤的那一幕啊!(呼……这句话定语好长啊~~~)  偶一听没有里维就在嘲笑,哼!删掉里维偶看最后是谁搀扶席巴去见被困的多利亚斯!万万没想到的是:竟然是里鲁康?!各位同人啊!无论从哪个角度想:一个万分自傲、执著、倔强的人(魔)会去帮助一个自己强X未遂的人么?别管是为了救谁。偶认为这处的改编完全使高贵的妖魔违背了自己的个性!正常来讲还是本编故事诠释得好,里鲁康就算帮也只会帮多利亚斯,而不会是席巴!因为是席巴害他那么惨:被君王冷落、被罗贤嘲笑、被死灵王利用、又被水魔王当棋子,最后还被疫魔王手下联合罗贤攻击……这样的他怎么可能去帮席巴呢?他若真再见到席巴也只可能再强X他一次,所以他宁愿选择永远的沉睡在艾莎体内,眼不见为静!各位看官觉得呢?  话又说回来,能沉睡在可爱少女体内不是每个男人的梦想吗?哈哈哈哈……  哦!如果真的有里维各位觉得哪位声优能胜任呢?偶个人是觉得森久保祥太郎很合适哦!他的声音明亮、清新、有侠义感!既能表现出和蒂亚在一起孩子般的淘气感;又能表现敢于冲入黑暗对抗多利亚斯帮助席巴的勇气;同时,蒂亚死后的伤感他绝对能胜任!(这点通过《纯情BOY禁猎区》中他出演的浅香律耍宝的顽皮、到帮助浅香纯和老师柳生流一郎在一起的侠义、以及暗恋英的伤感就能证实了!)粉合适的感觉!嗯……不过偶想妖魔封印CD出版的时候森久殿下大概还不知道在哪里上学前班了吧……哎~~岁月~~  5.对12卷书只有3张Drama CD的不满!  很多CD追得很辛苦,就是因为不停地出,而且越到后面情节就越勉强,这里要点名批评一下《幸せにできる》(《会幸福的》)。拼凑剧情的程度直追午间肥皂剧!附赠的一整张特典CD只有5分多钟啊!太骗人了!  哦!跑题了!继续说《妖魔的封印》:  偶当时想,书都有12卷CD至少有10张吧,谁知道接到的货只有3张,偶问了卖家好几遍:“只有3张么?没错么?”  卖家很果断地告诉偶说:“不会错!难道我有钱不赚么?!”  偶一时受不了,偶守候了这么多年的漫画,只用3张碟就完全收录了么?一听之下才晓得删了这么多啊!  举个反例,就比如说《KIZUNA》漫画出了10卷,本编CD出到1-5其中2-5都是2枚组!这一下子就是9张,再加上3张VCD,1张SPECIAL,1张现场版FAKEなKIZUNA,竟达到了14张!并还没有完结!情节100%保留!(偶光跟买《KIZUNA》就差点破产!)哦!为何同样是经典作品却对待如此不同呢?  或者为什么《妖魔的封印》不OVA化,或重新录制CD呢?  偶想这多少和盐大的死有关吧?莫非是太过伤感而没有人愿意再提起或触碰盐大曾饰演过的角色呢?还是没有人能替代盐大的地位,而无法再重录吧……  偶的声乐老师曾经说过:“每个人其实也是一件乐器,每个人的声音都是独特的,就算别人能模仿他人的声音也无法完全替代那个人。”这句话用在盐大身上无疑是再合适不过了!  但愿盐大在天堂依然能用他的嗓音奏出更美的旋律,人世间失去的东西,但愿能在天堂中永恒……一这么想就觉得这分离只是暂别,我们于是什么都没有失去,我们的付出是天堂的获得,而我们也终会将其重新获得。  偶相信,那些能用声音带给我们美好享受的人本来就来自某个天堂,偶感谢有那样一个地方的存在!  6.几大心目中的经典:  (1)最经典的改编对白:  被撕去脸后的席巴正在痛苦,来了一个人搀扶他去见被困的多利亚斯,  席巴问:“你是谁?”  里鲁康答:“我是谁无所谓……”  偶认为这对白改得最经典,不改的话简直没办法,各位觉得里鲁康是不是真正想说的是:  “我是以前想强X你的人,你可能不记得了,没关系,反正我也没做成……”真默~~~  (2)对井上和彦作为疫魔王选择的到位!  记得上次看碟评提到,井上大人对角色诠释总是很“准”,我觉得与其说准,不如说井上大人所有的角色都有相似点!他的所有角色都有那么一股“邪”!而他根本就是个有邪气的人!对他有这种印象的证据就是《纯情BOY禁猎区》中“野兽老师”柳生流一郎和《拥抱春天》中“人妖欧吉桑”佐和渚……等等形象!  当然还有他本人就是那种让女生尖叫而绝对不能托付终身的坏、酷、帅男人的形象!否则的话他也不会一连结了四次婚啊!!天哪!!  真是问题人物!  一这么想就觉得,果然多利亚斯对拉瑞尔的对白就可以照搬为对井上大人的经典评价一条——  “你走吧,只要有你在就能让全首都的人都得病。”  (3)很感动多·席二人再相爱:  席巴打开拉赛亚困住多利亚斯的门,带着哭腔和浓浓的爱意与相思呼唤的那两声:“多利亚斯,多利亚斯!”让偶整个心温暖起来,眼眶一下子酸涨不已!不觉在心底轻叹一声:太好了,终于,终于啊!  偶不知道日本的作家为什么,就好象有共识一般偏爱悲剧或惨剧!其实塑造一个被读者认可的形象容易么?为什么总让他们受伤或死去才开心呢?偶真搞不懂!《银之镇魂歌》的阴影长久地在偶心头萦绕不退!每每想起总有窒息之感!当然偶最不能容忍的还是让角色肢体伤残!《绝爱》中的泉与晃司,哦不!神样!请将这二人从作者尾崎南的“魔爪”中解救出来吧!  如泉所说:“我已经不想再看到有人死去了!”  偶想说的是:“我已经不想再看到有人受伤了!”  就更不用说《间之楔》中,哎!竟然都让角色死前还被砸断了腿,哦不……  而《妖魔的封印》,偶却认可了席巴的死亡!答案很简单,席巴的死是为了来世的生!是命运给予的考验,是为了最终二人以更好的方式、更坚强的心、以及更完善的自我结合在一起的考验吧!所以,这是偶最喜欢《妖魔的封印》这部作品的原因,这部作品真的做到了所谓——在绝望中存在希望!  希望我们每个人也能在这部作品中见证角色永恒之爱的同时得到力量!!  感谢各位看官耐心地将文章读完,恭喜看官又用眼睛扫描了4000多个字哦!下回见!拜拜~~

Come With Me 歌词

歌曲名:Come With Me歌手:Triumvirat专辑:Russian RouletteSpecial D - Come with Mecome with meAt the end of every weekeach one of us becomes a freak.Tonight the dj makes us move and thenthe sweat drops from the roof.Each time you let the bass beat hard to know we all spend now apart.Just activate your energy lets sing the song and come with me.The speaker system brings the sound,And light effects start spinnin' round.All the people on the floor theyshake their bodies cry for more.Never let this feeling go and let the music take control.Forget your problems and be free,Enjoy this moment, come with me!At the end of every week each one of us becomes a freak.Tonight the dj makes us move and then the sweat drops from the roof.Each time you let the bass beat hard to know we all spend now apart.Just activate your energy lets sing the song and come with me.The speaker system brings the soundAnd light effects start spinnin' round.All the people on the floor they shaketheir bodies cry for more.Never let this feeling go and let the music take control.Forget your problems and be freeenjoy this moment, come with me!Turn it up!Come With ,come with mecome with ,come with meCome with ,Come with meCome,come,come on baby come with meCome With,Come with mecome,come,come on babycome with meThe speaker system brings the soundAnd light effects start spinnin' round.All the people on the floor they shaketheir bodies cry for more.Never let this feeling go and let the music take control.Forget your problems and be freeenjoy this moment, come with meTurn it up!At the end of every week eachone of us becomes a freak.Tonight the dj makes us move andthen the sweat drops from the roof.Each time you let the bassbeat hard to know we all spend now apart.Just activate your energy lets singthe song and come with me.The speaker system brings the soundAnd light effects start spinnin' round.All the people on the floor they shaketheir bodies cry for more.Never let this feeling go and let the music take control.Forget your problems and be free enjoy this moment, come with mecome with, come with mecome with, come with mehttp://music.baidu.com/song/2789647

A generation gap has become a serious problem关于这的英语完形填空

A generation gap has become a serious problem. I read a report about it in the newspaper.Some children have killed themselves after quarrels with parents,I think this is because they don"t often have a talk with each other.Parents now spend more time in the office,so they don"t have much time to stay with their children.As time passes,they both feel that they don"t have the same topics to talk about.I want to tell parents to be more with your children,get to know them and understand them.And for children,show your feelings to your parents.They are the people who love you.So tell them your thought.In this way,you can have a better understanding of each other.

棋魂里面所有的歌曲 用文件夹发给 给你加上50分 lifang217@qq.com

发了

A generation gap has become a serious problem关于这的英语完形填空

A generation gap has become a serious problem. I read a report about it in the newspaper.Some children have killed themselves after quarrels with parents,I think this is because they don"t often have a talk with each other.Parents now spend more time in the office,so they don"t have much time to stay with their children.As time passes,they both feel that they don"t have the same topics to talk about.I want to tell parents to be more with your children,get to know them and understand them.And for children,show your feelings to your parents.They are the people who love you.So tell them your thought.In this way,you can have a better understanding of each other.

http://v.youku.com/v_show/id_XMTU5OTUzMzYw.html 第一首歌名

第一首:Brown Eyed Girls------Abracadabra帮你找到混合成这个视频的三个其他视频,右边视频信息里有这些歌的介绍,好好看吧!http://v.youku.com/v_show/id_XMTY0NDExODI0.html(这个就是第一部分的视频所用歌曲包括:rudenko - everybodykraftwerk - tour de france2ne1 - firebrown eyed girs - abracadabrablack eyed peas 黑眼豆豆 - boom boom powIU - boo4minute - hot issuedj jazzy jeff n fresh prince - boom shake the roomt-ara - 놀아볼래steady b - ding ding dingElize - i can be a bitchfrench affair - heart goes boomsnsd - 소원을 말해봐wonder girls - tell me배슬기 裴涩琪 - 지겨워snsd - chocolate lovef(x) - chocolate lovesandara - kiss이현지 李贤智 - kiss meprince - kiss우이경 禹怡京 - 2% 부족한사이Katy Perry 凯迪派瑞 - I kissed a girlt-ara - bo peep bo peepsnsd - geesuper junior - sorry sorryboa - i did it for loveparadiso girls - patron tequilaboa - energeticwonder girls - 쪼요쪼요f(x) - chuesmee denters - outta here우이경 禹怡京 - 나만봐kara - 똑같은맘rainbow - gossip girl소리 Sori - 입술이 정말leva"s polkakoyote - 아 정말2ne1 - let"s go partyrudenko - everybodystacey q - two of heartsbritney spears 布兰妮 - 3duke - party tonightkurtis blow - aj scratch윤하 尹河 -123rihanna 蕾哈娜 - russian rouletteafter school - 너때문에g-dragon - heartbreakerbrand new day - mascara 한예서 - No.0u-kiss - 만만하니shakira - she wolflady gaga - poker facemichael jackson 迈克尔杰克逊 - billie jeanpussycat dolls - don"t chalady gaga - just danceshinee - ring ding dongfrench affair - ring ding dongtomb raider samplejewelry - varietyafter school - ah손담비 孙丹菲 - 토요일밤에IU - 미아한다연 韩佳人- 어어어brown eyed girls - signdevil n angel - bad girl2pm - again n againbrown eyed girls - candyman손담비 孙丹菲 n after school - amoledciara - love sex , magic채연 蔡妍 - 흔들려 摇摆씨야 다비치 지연 - 여성시대N.S 윤지 允智 - 머리아파ciara - workthe saturdays - workk-will - 눈물이 뚝뚝seeya - 그놈 목소리)http://v.youku.com/v_show/id_XMTYzNzQ2Njky.html(这个已经打不开了)http://v.youku.com/v_show/id_XMTQxMzMyMjA0.html (这是后面的视频)

sql中创建表的时候,为什么not null要放在comment后面呢?

应该是sql规定的语法格式,记住就行了

新目标英语九年级 U5~U6听力原文 513612935@qq.com 谢谢了。

Unit 5 It must belong to Garla.Section A1b Listen and match each person with a thing and a reason.Girl 1: Whose volleyball is this!Boy 1: It must be Carla"s. She loves volleyball.Girl 1: How about this toy car?Girl 2: Oh, that toy car must belong to Jane"s little brother. He was the only little kid at the picnic. And the magazine must belong to Deng Wen. He loves cats.Boy 1; Oh, and look, someone left a book.Girl 2; Oh, yeah...This book must be Mary"s. Hemingway is her favorite author.Girl 1: OK...and how about this CD?Girl 2: Hmmmm...The CD must belong to Grace. She always listens to classical music. 2a Bob and Anna found a backpack in front of their school. Listen and write down the things in the backpack.Bob; Oh, look! Whose backpack do you think this is?Anna; I don"t know. Look, here"s a school T-shirt.Bob: Well then, the person must go to our school. Oh! Here is a hair band, so the person can"t be a boy.Anna: It could be Mei"s hair band. She has long hair.Bob: Or the hair band might belong to Linda. She was at the picnic, wasn"t she?Anna: Yes, she was. But then the backpack could belong to Rita. She"s always forgetting things.Bob: Oh, look! Tennis balls.Anna: Then it must be Linda"s backpack. She has long hair and she"s on the tennis team.Bob: You"re right!2b Listen again. Fill in the blanks Section B2a Listen and number the pictures [1-3] above. Then write two or three sentences to finish the story. Man: Hey, look at the man running down the street. I wonder what"s happening.Woman: He could be running for exercise.Man: But he"s wearing a suit.Woman: Well, he might be late for work.Man: He looks kind of scared.Woman: Oh, no! What"s that?Man: Where?Woman: there"s something in the sky.Man: It could be a helicopter.Woman: No, it can"t be a helicopter. It"s too big. It must be a UFO.Man: A UFO? What"s going on?Woman: Look, now the UFO is landing...Man: And there"s a strange creature getting out. It must be an alien.Woman: And the alien is chasing the man! I must be dreaming.Man: May be we should call the police. Hey, wait a minute. What"s that?Woman: Where?Man: Over there.Woman: It"s a woman with a camera.Man: She could be from the TV news.Woman: No, look at all those other people .They"re actors. Oh! They must be making a movie.2b Listen again. Complete the sentences.Review of units 1-52a Listen to the four conversations. Match the conversations with the correct pictures.Conversation 1Chen: Zhu, is that you?Zhu: Yes. You are Chen, aren"t you?Chen: Yes, that"s right. You"ve changed a lot!Zhu: Really? How?Chen: You used to be short. Now you"re tall.Zhu: Yes, I am.Chen: You used to have long hair. Now you have short hair.Zhu: Yes, that"s right.Chen: And you used wear glasses.Zhu: Yes, I still do. But today I have contact lenses.Chen: You used to play the guitar, didn"t you?Zhu: No, I played the piano. My friend Xiao Li played the guitar.Chen: Oh, yes. I forgot.Conversation 2Ben: I"m doing a survey. Can I ask you some questions?Boy: Sure.Ben: What would you do if you could be a big bicycle accident?Boy: I would call the police.Ben: What would you do if you could be a famous doctor?Boy: I would help people who can"t see.Ben: What would you do if you could fly anywhere in the world?Boy: Mm. That"s hard. There"re lots of places I"d like to see. I"d go to Canada.Ben: Good. One more question. What famous person from history would you most like to meet?Boy: I would most like to meet Zhou Enlai.Ben: Good answer. That"s all my questions.Conversation 3Lisa: Oh, ma. I can"t learn English.Ma: No? What"s the problem?Lisa: I read my books but I always forget. How do you study?Ma: By using flashcards. But everyone is different. Tony doesn"t like flashcards.Lisa: How does he learn when he doesn"t understand?Ma: By asking the teacher. But maybe you should learn English like Judy. She"s the best student.Lisa: How does Judy learn?Ma: By watching English TV.Lisa: Really? How about Ping? Is there another way she learns?Ma: Yes. By studying grammar.Lisa: I don"t like grammar.Ma: Well, everyone learns a different way.Conversation 4Henry: Hi, Kitty. Are you going to the party tonight?Kitty: No. I"m not allowed.Henry: You"re allowed to go to party, aren"t you?Kitty: No. There are lots of things I can"t do.Henry: Like what?Kitty: Well, I"m not allowed to watch TV on school nights.Henry: Really? But there are lots of good shows. You can learn a lot.Kitty: Also, I"m not allowed to shop for clothes. My mother buys them.Henry: Do you parents do everything for you?Kitty: No, but they have lots of rules.Henry: Are you allowed to have a pet?Kitty: Oh, no! I"m not allowed to have a pet.Henry: What are you allowed to do?Kitty: My parents say I"m allowed to study all night. Their rules make me a good student.2b Listen again. Follow the instructions next to the pictures.Unit 6 I like music that I can dance to.Section A1b Listen and check the kinds of music Tony and Betty like.Betty: Oh, look. There"s the new Cool Kids CD.Tony: The Cool Kids? Do you like them?Betty: Oh, yeah. They"re my favorite band. I like music that I can dance to.Tony: You"re kidding. I think they"re awful. I prefer music that has great lyrics.., music that I can sing along with.Betty: I like songs I can sing along with too. So what"s your favorite band?Tony: The Lions. Their words are interesting and...2a Listen and circle “T” (for true) or “F” (for false).Boy: Look, Carmen. These T-shirts are great! Look at this one.Girl: What a great T-shirt, Xu Fei. I really love Dan Dervish. I like musicians who play different kinds of music.Boy: Hmmm... he"s okay....Girl: He"s only okay?! You must be joking.Boy: Well... I like musicians who write their own songs. Dan Dervish doesn"t write his own music.Girl: Hmm. Well, I think he"s great.Boy: The Modern"s T-shirt is interesting.Girl: The Modern are really great. I love music that"s really loud and energetic.Boy: I know you do... but I prefer groups that play quiet and gentle songs.2b Listen again. Complete the sentences.Section B2a Listen and write down the three things in the picture that Michael and Ali talk about.Boy 1: Wow, you sure have lots of cool stuff. What a great jacket!Boy 2: Yeah, it"s new. I really like it. I like clothes that are unusual.Boy 1: Yeah, me too. Say ... is that a new book over there?Boy 2: Yes, it is. It"s a book about volleyball. It tells about how to become a good player.Boy 1: Is it good?Boy 2: Yeah, it"s great. I like writers who explain things well.Boy 1: Me, too. Ummmm, Michael?Boy 2: Yes?BOY 1: Uhhh ... Where did you get that movie poster?Boy 2: Oh, the Monster Lizard poster? My brother got it for me. He works at a movie theater.Boy 1: It"s a great poster.Boy 2: Yeah, it is. I love movies that are about monsters, don"t you?Boy 1: I sure do. Say ... Michael ... Do you think your brother could get a Monster Lizard poster for me?Boy 2: Probably. I"ll ask him.Boy 1: That would be great.2b Listen again. Write what Michael likes and why he likes each thing.

as long as it is a comedy,i’d rather cry during the process.什么意思

As long as it is a comedy,i"d rather cry during the process只要是个喜剧结局,过程你让我怎么哭都行。

In that comedy ,all the toys in the toy store come______ A live B alive C alives D lives

B,B的意思是在那个喜剧里,所有的玩具都在这个玩具店里活了起来 。而A是在那个喜剧里,所有的玩具都在这个玩具店里存活。C则语法错误,不可能。D在那个喜剧里,所有的玩具都在这个玩具店里生活。应该是对的吧

《TheJervaiseComedy》epub下载在线阅读,求百度网盘云资源

《The Jervaise Comedy》(J. D. Beresford)电子书网盘下载免费在线阅读资源链接:链接:https://pan.baidu.com/s/1WuGRcWzl_rlfs8HAtvPHLA 提取码:8d79书名:The Jervaise Comedy作者:J. D. Beresford出版社:Book Jungle出版年份:2009-07-01

My sister wants to see the comedy变一般疑问句

Dose your sister want to see the comedy?

求歌词和翻译 The Silent Comedy乐队的bartholomew

Oh my god主啊,Please help me, knee deep in the river tryin" to get clean请救救我,我双膝埋在河流中试图洗清污浊。He says wash your hands, get out the stains他说:“洗洗你的手,把污秽除净。But you best believe, boy, there"s hell to pay但你最好相信,孩子,血债终会血偿。Yeah you best believe, boy, there"s hell to pay.是的你最好相信,孩子,血债终会血偿。“Oh my god主啊,Please help me, waist deep in the river, can you hear my plea?请救救我,河水没过了我的腰际,你能听见我的哀求吗?He says, son, you come like a beggar in the streets他说:“孩子,你像个街头乞人,You might make it, boy, but by the skin of your teeth*你也许能挺过去,孩子,但希望渺茫。You might make it, boy, but by the skin of your teeth你也许能挺过去,孩子,但希望渺茫。”I rambled with the worst of them 我曾与暴徒同行,Fell in love with a harlequin*爱过一条毒蛇,Saw the darkest hearts of men见过最黑暗的人性,And I saw myself starin" back again然后,在其中我看到了我自己,And I saw myself starin" back again在其中我看到了我自己。Oh my god主啊,Please help me, neck deep in the river screamin" for relief请救救我,河水已漫过我的脖颈,我哀鸣着企求解脱,He says, it"s mine to give, but it"s yours to choose他说:“我只给予,而由你自己做选择。You"re gonna sink or swim, you"re gonna learn the truth你将溺毙或是将浮起,你都将学到真理。No matter what you do you"re gonna learn the truth不论你如何选择,你都将学到真理。”Ate the bread that once was stone我吃过曾如石块的面包,Fell from a cliff, never broke a bone掉下悬崖而毫发无损,Bowed down to get the kings overthrown欠身之间倾覆王朝,And I"m all alone and the fire grows而如今我孤独一人,野火燎原And I"m all alone and the fire grows而如今我孤独一人,野火燎原Swing sweet charity歌颂那仁慈,Take what"s left of me带走我所剩,A new beginning or is this the end?这是新的开始或是结束?Swing sweet Seraphim*歌颂那天神,Take me back again带我回去,or watch me make the messes of men或是看我在人间妄行。

《The Comedyof Errors》txt下载在线阅读全文,求百度网盘云资源

《The Comedy of Errors》(William Shakespeare)电子书网盘下载免费在线阅读链接: https://pan.baidu.com/s/1KxUaXWIUCe0XHrU1EPqW6g 提取码: icqg书名:The Comedy of Errors作者:William Shakespeare豆瓣评分:7.5出版社:Cambridge University Press出版年份:2009-7-20页数:160内容简介:John Dover Wilson"s New Shakespeare, published between 1921 and 1966, became the classic Cambridge edition of Shakespeare"s plays and poems until the 1980s. The series, long since out-of-print, is now reissued. Each work is available both individually and as a set, and each contains a lengthy and lively introduction, main text, and substantial notes and glossary printed at the back. The edition, which began with The Tempest and ended with The Sonnets, put into practice the techniques and theories that had evolved under the "New Bibliography". Remarkably by today"s standards, although it took the best part of half a century to produce, the New Shakespeare involved only a small band of editors besides Dover Wilson himself. As the volumes took shape, many of Dover Wilson"s textual methods acquired general acceptance and became an established part of later editorial practice, for example in the Arden and New Cambridge Shakespeares.

C# combobox 取值问题

直接用this.brandBox.Text就可以了 不要用 this.brandBox.SelectedText

The comedy has _dialog which is very humorous

a

The Comedy of Errors是什么意思

The Comedy of Errors错误的喜剧The Comedy of Errors错误的喜剧

I completed Vice City and all I got was this lousy T-Shirt !是什么意思?

我闯过了罪恶都市这款游戏,但我却得到这件难看的T恤!

灼眼的夏娜s01中20分26秒的插曲是什么? 网址http://v.ku6.com/show/Jkgqdsyap1i8M6-S.html 谢……

All in good time 。。。。。。

www.nta777.com怎样激活太太乐积分

到经销商那里可以联系到当地太太乐餐饮业务员,也可以自己到NTA777网站注册会员,由当地太太乐餐饮业务员审核后开通.并激活你的积分,你就可以在NTA777网站上换商品 麻烦采纳,谢谢!

www.nta777.com怎样激活太太乐积分

到经销商那里可以联系到当地太太乐餐饮业务员,也可以自己到NTA777网站注册会员,由当地太太乐餐饮业务员审核后开通.并激活你的积分,你就可以在NTA777网站上换商品

电脑型号 海尔 HaierComputer All in one PC 台式电脑 这个配置好么 求大神

这个配置属于中低的 不怎么样.装win7的比较卡.上网.登QQ还可以.

电脑型号 海尔 HaierComputer All in one PC

要看具体的硬件配置情况才能确定。

海尔 HaierComputer All in one PC 玩游戏太卡了,需要换什么配置

这台电脑是笔记本内存还是台式机内存啊??

move come arrive leave go 用现在进行时表一样 什么意思 怎么理解

普通单词

company institution organization的区别

organization指所有具有组织形式的机构,包括institution;而institution专门用来指教育、科研、文化等上层建筑领域里的organizations。一个商贸公司可以是organization,但不是institution;但一个大学是一个institution,同时也是organization。

Community与Organization有什么区别

Community:n. 社区, 社会, 团体 非洲经济共同体African Economic CommunityOrganization:n. 组织, 结构, 团体, 体制北大西洋公约组织(北约)North Atlantic Treaty Organization (NATO) community和organization是不同的分类方法,还有usergroup,互相之间没有关系,可以认为usergroup和organization都是一种community

http://www.mogao.com.cn/摩高官网这首歌的名字?

是En Vogue的Free Your MindI wear tight clothing, high heel shoesIt doesn"t mean that I"m a prostitute (no, no, no)I like rap music, wear hip hop clothesThat doesn"t mean that I"m out sellin" dope (no, no, no)Oh my, forgive me for having straight hairIt doesn"t mean there"s another blood in my heirsI might date another race or colorIt doesn"t mean I don"t like my strong black brothers(Oh, oh, oh, oh)Why, oh why must it be this way?(Oh, oh, oh, oh)Before you can read me you got to learn how to see me(I said)Free your mind and the rest will followBe color-blind, don"t be so shallowFree your mind and the rest will followBe color-blind, don"t be so shallowSo I"m a sistaBuy things with cashThat really doesn"t mean that all my credit"s bad (Oh)So why dispute me and waste my timeBecause you really think the price is high for meI can"t look without being watchedYou rang my buy before I made up my mindOh now attitude why even botherI can"t change your mind, can"t change my color(Oh, oh, oh, oh)Why, oh why must it be this way?(Oh, oh, oh, oh)Before you can read me you got to learn how to see me(I said)Free your mind and the rest will followBe color-blind, don"t be so shallowFree your mind and the rest will followBe color-blind, don"t be so shallowFree your mind and the rest will followBe color-blind, don"t be so shallowFree your mind and the rest will followBe color-blind, don"t be so shallowFree your mind(Oh, oh, oh, oh)Why, oh why must it be this way?Before you can read me you got to learn how to see me(I said)Free your mind and the rest will followBe color-blind, don"t be so shallowFree your mind and the rest will followBe color-blind, don"t be so shallowFree your mind and the rest will followBe color-blind, don"t be so shallowFree your mind and the rest will followBe color-blind, don"t be so shallow

生物专业词汇"nuclear compartment”是什么意思?

nuclearcompartment核间隔相关例句:1.Thispaperpresentsanexperimentalstudydealingwiththeburningrateofpoolfireinaconfinedandventilatedcompartmentwithintheframeworkofnuclearsafetyresearch.在核安全研究框架内对在受限和通风防火分区内油池火燃烧速率进行了试验研究。

company 和 organization的区别

一个是公司,一个是组织。

我在dos下运行lzma.exe时提示 Error:incorrect command 这是怎么

有两种方式。1.在dos界面中进入lzma.exe的所在目录,然后再输入lzma.exe。2.把lzma.exe所在目录添加到环境变量path中,这样无论在哪个目录下都可以执行。有问题再追问,望采纳。

求一首歌的罗马音 地址http://www.xiami.com/song/1774030253

春の雪 ヒラヒラと 一片の想いを隠してharu no yuki hirahirato hitohira no omoiwo kakushiteいつまでも いつまでも 届かない浅い梦のようにitsumademo itsumademo todoka nai asaiyume no youni小说はここで终わりshousetsu wa kokode owari缲り返し読みすぎて 擦り切れたぼろいページkurikaseshi yomi sugite surikireta boroi pe-jiもう舍ててしまおうかなmou sutete shimaou kana出来もしないことさえも はしゃいで追いかけた日々dekimo shinai koto saemo hashaide oikaketa hibi制服の仆たちが この道を駆けていくseifuku no bokutachi ga kono michiwo kakete yukuつまらない见栄と意地と 后悔だけ覚えてtsumara nai mieto ijito koukai dake oboete“上手く大人になれたかい?”umaku otonani naretakaiどうか応えてくれdouka kotaete kure会いたくて 会いたくて 気がつけばまた春の匂いaitakute aitakute kigatsukeba mata haruno nioi何度でも 何度でも すり抜けていく君の面影nandodemo nandodemo surinukete iku kimino omokageそうずっと もうずっと 呼び続けているsou zutto mou zutto yobitsudukete iruいつかは消えてしまうからitsukawa kiete shimau kara返す机会失った言叶も あの楽谱もkaesu kikai ushinatta kotobamo ano gakufumo时が止まった仆さえも どこにたどり着くだろうtokiga tomatta boku saemo dokoni tadoritsuku darou水面が白く染まって 同じ季节が巡るよminamoga shiroku somatte onaji kisetsuga meguruyo“ちゃんと幸せになれたかい?”chanto shiawaseni naretakaiいつか教えてくれitsuka oshiete kure春の雪 ヒラヒラと 一片の想いを包んでharu no yuki hirahirato hitohira no omoiwo tsutsundeいつまでも いつまでも 届けたい君のその空さえitsumademo itsumademo todoketai kimino sorasaeそうずっと もうずっと 流れてた时间sou zutto mou zutto nagareteta jikanいつかは死んでしまうからitsukawa shinde shimau karaたった一度きりの花はやがて枯れてもtatta ichidokiri no hanawa yagate karetemo春が来る度にまた逢えるというらしいharuga kurutabini mata aeru toiu rashii人ごみの街中でもこの桜の川辺でもhitogomino machinaka demo konosakura no kawabe demo平等に访れる终わりと始まりを 优しく濡らしていくbyoudouni otozureru owarito hajimariwo yasashiku nurashite yuku春の雪 ヒラヒラと 一片の想いを许してharuno yuki hirahirato hitohirano omoiwo yurushiteいつまでも いつまでも 届けたい君の空までitsumademo itsumademo todoketai kimino soramade会いたくて いま会いたくて 泣き出しそうなこの春の匂いaitakute ima aitakute nakidashisou na kono haruno nioi何度でも 何度でも すり抜けてく君の幻nandodemo nandodemo surinuketeku kimino maboroshiそうずっと もうずっと 呼び続けているsou zutto mou zutto yobitsudukete iruいつかは忘れてしまうからitsukawa wasurete shimau karaうわごとのように 君の名前をuwagoto no youni kimino namaewo

Incorrect email/password combination.什么意思

Incorrect email/password combination.不正确的电子邮件/密码组合。

解压文件时出现incorrect command line,请问如何解决?

WIN7:控制面板-查看方式选大图标-默认程序-将文件类型与协议与程序关联-选中7Z或RAR-更改程序-7ZIP file manager

Theoritical computer science 怎么样

  Theoretical Computer Science is mathematical and abstract in spirit, but it derives its motivation from practical and everyday computation. Its aim is to understand the nature of computation and, as a consequence of this understanding, provide more efficient methodologies. All papers introducing or studying mathematical, logic and formal concepts and methods are welcome, provided that their motivation is clearly drawn from the field of computing.

英语all 用法的疑问, Let us all sing a song. You can all come in.

Let us all sing a song. You can all come in. 请问上面2个句子的all是修饰主语。第一句all不能放在us前面,加上of后,all of us。第二句all不能放在can前面,be动词之后,行为动词之前。

求一首英文歌,劲歌很high的那种,只记得里面有一句come on baby xxxx英文不好啊,不会听歌词

I believe I Swear IF - bread if you were here imagine - eva cassidy in my pocket - mandy moore come on over - christina aguilera Don""t cry(original) - guns and roses Don""t make me wait - 911 dreams come true dumb - nirvana earth song - michael jackson Earth song - Michael Jackson everyone - backstreet boys everything i do(i do it for you) - bryan adams eyes on me(final fantasy - 王菲 fields of gold - sting first love - 宇多田光 fly me to the moon - 新世纪福音战士片尾曲 绵羊物语 goddess in the doorway goodbye""s (the saddest word) - celien dion goodbye - air supply Happy New Year heal the world - michael jackson heal the world - michael jackson hear me cry - cagnet hear me cry - cagnet hotel california - the eagles hotel california - the eagles Big Big World - emilia Almost Over You - sheena easton As Long As You Love Me - backstreet boys Baby Onemore Time - Britney Back At One - westlife ruru because of you(perfect) - 98 degrees Bizarre love triangle - frente It"s true - backstreet boys black or white - Michael Jackson Born In Africa Bressanone - matthew lien brothers in arms - dire straits butterfly - smile can you feel the love tonight - elton john Casablanca - bertie higgins I want a fat babe it""s gonna be me - n""sync I""ll Never Break Your Heart - Backstreet Boys jingle bells lemon tree listen to your heart - roxette mirror mirror - m2m more than i can say - leo sayer my heart will go on(我心永恒) - celine dion my little dankey my love - west life never grow old - the cranberries only love - trademark paint my love - michael learns to rock Pepsi - Joy of Cola perfect moment - martine mccutcheon - 流星花园 northern star - melanie c pretty boy - m2m pretty boy promiss don""t come easy - caron nightingale purple haze - Jimi Hendrix reality - richard sanderson Rhythm of the rain - the cascades right here waiting - richard marx sailing - rod stewart save the best for last - vanessa williams Scarborough fair - paul simon season in the sun - westlife shape of my heart - backstreet boys smoke gets in your eyes - julio iglesias song from A secret garden speak softly love(教父主题曲) - andy williams southwest CADiLLAC take a look around - limp bizkit tears in heaven - eric clapton thank you - dido that""s why(you go away) - j.richter the call - backstreet boy The day you went away the Day you went away the day You went away There You′l Be(电影『珍珠港』英文主题曲) - faith hill 千万次的问 - 刘欢 top of the world - carpenters uptown girl - westlife walk in the rain what will I do when I dream of you windflowers without you - mariah cary yellow - coldplay yesterday once more - carpenter yesterday once more yesterday once more you""re my sunshine You Driver Me Crazy

Comatose (In The Arms Of Slumber) 歌词

歌曲名:Comatose (In The Arms Of Slumber)歌手:Eagle-Eye Cherry专辑:DesirelessComatoseSkilletComatoseI hate feeling like thisI"m so tired of trying to fight thisI"m asleep and all I dream ofIs waking to youTell me that you will listenYour touch is what I"m missingAnd the more I hide I realize I"m slowly losing youComatoseI"ll never wake up without an overdose of youI don"t wanna liveI don"t wanna breathe"les I feel you next to meyou take the pain I feelwaking up to you never felt so realI don"t wanna sleepI don"t wanna dream"cause my dreams don"t comfort meThe way you make me feelWaking up to you never felt so realI hate living without youDead wrong to ever doubt youBut my demons lay in waitingTempting me awayOh how I adore youOh how I thirst for youOh how I need youComatoseI"ll never wake up without an overdose of youI don"t wanna liveI don"t wanna breathe"les I feel you next to meyou take the pain I feelwaking up to you never felt so realI don"t wanna sleepI don"t wanna dream"cause my dreams don"t comfort meThe way you make me feelWaking up to you never felt so realComatoseI"ll never wake up without an overdose of youI don"t wanna liveI don"t wanna breathe"les I feel you next to meyou take the pain I feelwaking up to you never felt so realI don"t wanna sleepI don"t wanna dream"cause my dreams don"t comfort meThe way you make me feelWaking up to you never felt so realBreathing lifeWaking up My eyesOpen upDon"t leave me alonehttp://music.baidu.com/song/8040803

we hope you can come to the party tonight

We hope you will be able to come to the party tonight

Mr Green is busy today.He ______ to our party tonight.(not come)

我们也是相同类型的题目哦,明天就会知道答案,明天我会告诉你!

Tom ﹙ ﹚come to the party tonight. but I am not sure about it. A.need B.must C.may D.would

C

Can you come to my house to have a party tonight

选第二个。。Sure,but I have to visit my teacher

一NaJlcy is not coming to the party tonight. --But she__________me she‘d love to.

【答案】:BNcacy告诉我她想去这件事是发生在过去的,所以应该用tell的过去式形式told。

韩国成人打砖块,是个FLASH游戏,你给别人回答过,不过是邮箱 给我也发一个吧,mashimaro123@163.com,谢啦

麻烦发一个谢谢249012821@qq.com,谢谢哈

one component system 单因子系统 是什么

单组成因素生产系统(one-component system):所有的组成成分都集中在生产细胞中。经典的反转录病毒生产系统就是由产病毒细胞(VPC)组成,重组反转录病毒由VPC细胞不断分泌至培养上清中。这种生产系统操作最为简单,但是往往产量不高或不稳定。采用这种策略,需要将重组病毒产生所需要的所有元件都稳定地置于生产细胞中。由于许多病毒基因产物本身对细胞有破坏作用或不能在细胞中稳定表达,因此这种策略在许多病毒载体的生产中难以实施。第一节 病毒载体产生的原理病毒载体的产生建立在对病毒的生活周期和分子生物学认识的基础之上。研究病毒载体首先要对病毒的基因组结构和功能有充分的了解,最好能获得病毒基因组全序列信息。病毒基因组可分为编码区和非编码区。编码区基因产生病毒的结构蛋白和非结构蛋白;根据其对病毒感染性复制的影响,又可分为必需基因和非必需基因。非编码区中含有病毒进行复制和包装等功能所必需的顺式作用元件。各种野生型病毒颗粒都具有一定的包装容量,即对所包装的病毒基因组的长度有一定的限制。一般来说,病毒包装容量不超过自身基因组大小的105~110%。基因重组技术的发展使病毒载体的产生成为可能。最简单的做法是,将适当长度的外源DNA插入病毒基因组的非必需区,包装成重组病毒颗粒。比如,本实验室曾将4.5kb的lacZ基因表达盒(CMV-lacZ-polyA)插入HSV1病毒的UL44(糖蛋白C)基因的XbaI位点中,病毒基因组的其余部分不改变,构建成重组病毒HSV1-lacZ100(吴小兵等,1998)。由于UL44基因产物对于HSV病毒在培养细胞中产毒性感染是非必需的,因此,该重组病毒可以在细胞中增殖传代。用这种重组病毒感染细胞,能将lacZ基因带入细胞并高效表达。用同样的方法,将AAV-2病毒的rep和cap基因片段(4.3kb)插入HSV1病毒的UL2(编码尿嘧啶DNA糖基化酶)或UL44(编码糖蛋白C)基因中,构建成具有提供重组AAV载体复制和包装所需的全部辅助功能的辅助病毒rHSV-rc(伍志坚等,1999)。然而,这样的重组病毒作为基因转移载体有许多缺点。首先,许多野生型病毒通过在细胞中产毒性复制而导致细胞裂解死亡;或带有病毒癌基因而使细胞发生转化。因此必须经过改造使其成为复制缺陷性病毒并且删除致癌基因后才能用于基因治疗。其次,插入外源DNA的长度受到很大限制,尤其对于基因组本身较小的病毒如腺病毒伴随病毒(AAV,4.7kb)、反转录病毒(8~10kb)、腺病毒(36kb),如果不去除病毒基因,可供外源DNA插入的容量就十分小。因此,必须删除更多的病毒基因以腾出位置插入较大的外源DNA。 为了增加病毒载体插入外源DNA的容量,除了可以删除病毒的非必需基因外,还可以进一步删去部分或全部必需基因,这些必需基因的功能由辅助病毒或包装细胞系反式提供。病毒载体大体上可分为两种类型: 重组型病毒载体:这类载体是以完整的病毒基因组为改造对象。一般的步骤是选择性地删除病毒的某些必需基因尤其是立早基因或早期基因,或控制其表达;缺失的必需基因的功能由互补细胞反式提供;用外源基因表达单位替代病毒非必需基因区;病毒复制和包装所需的顺式作用元件不变。这类载体一般通过同源重组方法将外源基因表达单位插入病毒基因组中。如在传统的重组腺病毒构建方法中,将外源基因表达盒(exogenous gene expression cassette)插入穿梭质粒(如pXCX2或pFGdX1)的腺病毒同源序列中,与辅助质粒(含有腺病毒基因组的质粒如JM17或pBHG)共转染293细胞,通过细胞内的同源重组获得含有外源基因的重组腺病毒(Graham FL and Prevec L 1995)。无病毒基因的病毒载体(gutless vectors):这类载体在不同的病毒载体系统中的称谓不同。对于腺病毒,一般称为mini-Ad;在HSV载体系统,一般称为扩增子(amplicon)载体或质粒型载体。重组AAV载体也属于无病毒基因的病毒载体。这类载体系统往往由载体质粒和辅助系统组成。重组载体质粒主要由外源基因表达盒、病毒复制和包装所必需的顺式作用元件及质粒骨架组成。辅助系统包括病毒复制和包装所必需的所有反式作用元件。在辅助系统的作用下,重组载体质粒(包含或不包含质粒骨架)以特定形式(单链或双链,DNA或RNA)被包装到病毒壳粒中,其中不含有任何病毒基因。这类病毒载体的优点在于载体病毒本身安全性好,容量大。缺点在于往往需要辅助病毒参与载体DNA的包装,而辅助病毒又难以同载体病毒分离开来,造成最终产品中辅助病毒污染,从而影响其应用。实际上,无病毒基因的病毒载体可以看作是重组病毒载体的一种极端减毒情况。表1列举了几种常见的病毒载体的顺式作用元件和经典的包装方式。 载体 顺式作用元件 经典包装方式 反转录病毒载体 1) 两个长末端重复序列(LTR):含有整合和调节转录的必需序列;含有转录增强子和启动子;2) tRNA 引物结合位点p;3)包装信号序列ψ。 载体质粒转染整合了所有必需基因(gag,pol,env)的包装细胞系如PA317,经选择培养获得产病毒细胞系(VPC);细胞不裂解,病毒不断分泌至培养上清中。腺病毒载体 两个末端倒转重复序列(ITR);包装信号序列。 载体质粒与辅助质粒共转染293细胞获得重组腺病毒。重组腺病毒感染293细胞而扩增;细胞每次被感染后发生裂解。腺病毒伴随病毒载体 两个末端倒转重复序列(ITR);其中包括了病毒复制起点、包装信号及整合和拯救所必需的顺式元件。 AAV载体质粒和辅助质粒共转染293细胞,并加辅助病毒(腺病毒或单纯疱疹病毒)感染。单纯疱疹病毒载体 HSV 病毒复制起点ori; 病毒包装信号pac 。 PTCA重组病毒在互补细胞上传代;扩增子病毒在辅助病毒存在下传代。返回标题第二节 病毒载体的包装系统将外源基因包装到病毒壳粒中,是病毒载体生产的核心技术。一般地,病毒载体的制备包括以下要素:宿主细胞虽然现在已有可能对有些病毒载体(如AAV载体)进行体外(无细胞)包装(Zhou XH et al . 1998; Ding L et al. 1997),但是这种包装系统仍然需要细胞提取物,并且包装效率相当低,远远达不到可生产水平。至今为止,病毒载体的包装主要是在对该病毒敏感的宿主细胞中进行的。宿主细胞不但提供了病毒复制和包装的环境条件,许多细胞成分还直接参与了病毒复制和包装的过程。病毒复制和包装所必需的顺式作用元件和外源基因的表达盒一般地,病毒复制和包装所必需的顺式作用元件和外源基因的表达盒由细菌质粒携带,组成病毒载体质粒,是被包装的对象。由于病毒复制方式的不同,有些病毒载体如单纯疱疹病毒扩增子(HSV amplicon)载体在包装时,整个载体质粒都被包装进入病毒颗粒中;而有些病毒载体如反转录病毒、腺病毒伴随病毒载体的质粒骨架部分并不被包装到病毒颗粒中,只有病毒复制和包装所必需的顺式作用元件和外源基因表达盒被包装到病毒颗粒中。构建重组型病毒载体时,病毒复制和包装所需的顺式作用元件存在于病毒基因组中(病毒基因组可以由具有感染性的病毒颗粒提供,也可以质粒形式提供)。先将外源基因表达盒插入穿梭质粒携带的病毒同源序列中;将重组穿梭质粒转染至细胞中,再用辅助病毒超感染;或将重组穿梭质粒与病毒基因组质粒共转染细胞;重组质粒与病毒基因组在细胞中进行同源重组而产生表达外源基因的重组病毒。 重组腺病毒(Graham FL and Prevec L 1995)和重组单纯疱疹病毒(Pyles RB et al. 1997;Kramm CM et al. 1997)的传统制备方法都是采用这种方式。为了使病毒载体的生产更为方便,病毒复制和包装所必需的顺式作用元件和外源基因的表达盒除了可以用质粒携带以外,也可以用另一种病毒(往往是辅助病毒)或生产细胞来携带。辅助元件包括病毒复制和包装所必需的所有反式作用元件。这些元件一般包括病毒基因转录调控基因、病毒DNA合成和包装所需的各种酶类的基因、病毒的外壳蛋白基因等。辅助元件的表现形式可以多种多样。常用的形式有:① 辅助质粒(helper plasmid),如用于产生重组腺病毒的质粒JM17,用于重组AAV包装的辅助质粒pAAV/Ad(Rolling F and Samulski J 1995)等;② 辅助病毒(helper virus),如用于HSV 扩增子载体包装的辅助病毒HSV1 tsK株;③ 包装细胞系如用于反转录病毒载体包装的PA317细胞。这些表现形式之间可以相互转化或合并。例如,辅助病毒可以转化为辅助质粒:传统的AAV载体生产系统常用腺病毒作为辅助病毒。研究发现,并非腺病毒的所有基因对AAV病毒的产生都是必需的,只需要腺病毒E1a, E1b, E2a, E4和VA RNA 5种基因就行了。因此,将这5种基因置于同一个质粒中,构建成的这种新的辅助质粒就完全可以替代原来的辅助病毒(Xiao X et al. 1998; Grimm D et al. 1998 ),不但提高了包装效率,而且避免了产品中腺病毒污染的问题。上述几种要素的不同组合,便产生了各种各样的病毒载体包装策略。根据病毒载体生产系统的组成因素的多少,可将其分成以下几种:单组成因素生产系统(one-component system):所有的组成成分都集中在生产细胞中。经典的反转录病毒生产系统就是由产病毒细胞(VPC)组成,重组反转录病毒由VPC细胞不断分泌至培养上清中。这种生产系统操作最为简单,但是往往产量不高或不稳定。采用这种策略,需要将重组病毒产生所需要的所有元件都稳定地置于生产细胞中。由于许多病毒基因产物本身对细胞有破坏作用或不能在细胞中稳定表达,因此这种策略在许多病毒载体的生产中难以实施。双组成因素生产系统(two-component system)这种生产系统一般由"一株病毒/一株细胞"组成。典型的例子是重组腺病毒生产系统。先用共转染的方法获得重组腺病毒毒种,再由该毒种和生产细胞(如293细胞)组成一个双组成因素的生产系统使病毒大量扩增。多组成因素生产系统(multi-component system)是由两种以上的组成因素组成的生产系统。传统的AAV载体生产系统就是由载体质粒,辅助质粒,辅助病毒和生产细胞4种因素组成。这种策略的缺点是影响因素多,操作复杂,产量不容易稳定,不利于大规模生产。 以上各种生产系统也可以相互转化。我们实验室通过将上述AAV载体生产系统的4种因素进行两两合并,即将辅助质粒和辅助病毒合并成一种重组的辅助病毒,将载体质粒和生产细胞合并成AAV前病毒细胞株,成功地将其转化成一种双组成因素的新型高效生产系统(伍志坚等,1999)。一般来说,发展新的包装策略主要是为了以下几种目的:(1) 减少生产系统中的组成因素,简化操作过程;(2)提高生产效率,降低生产成本;(3)避免或降低野生型病毒的产生;(4)避免使用难以与产品病毒分离的辅助病毒。返回标题第三节 病毒载体的纯化方法重组病毒的纯化与基因工程产品的纯化既有许多共性,又有显著的不同之处。病毒可以看作是一个具有特定结构的生物大分子,一般都由位于中心的核酸和包裹于其外的蛋白外壳组成。有些病毒还具有脂质膜和糖蛋白或糖脂。许多分离纯化蛋白质的方法如离心和梯度离心、盐析、柱层析、超滤、透析等方法都可用于病毒的纯化。然而,由于病毒结构的复杂性,纯化时不能采用蛋白质变性和复性的方法,因为一旦病毒蛋白发生变性,或病毒结构发生破坏,在体外就很难再重建成有感染性的病毒颗粒。因此,在病毒的纯化过程中必须保持病毒的结构不受破坏,并且监测其感染活性。病毒的纯化一般分为以下几个步骤:初始物(start material)的收集或制备根据病毒产生方式的不同而采取不同的方式。对于不导致细胞病变和死亡的病毒如反转录病毒,可不断收集产毒细胞(VPC细胞)的培养上清作为初始物;对于在生产病毒过程中宿主细胞发生病变和死亡的病毒如腺病毒,在病毒产生达到高峰时,收集培养上清,并裂解细胞以释放细胞内的病毒。培养上清和细胞裂解液都可作为纯化的初始物。在实验室的小规模制备中,往往采用将培养上清与细胞一起反复冻融3~4次的方法制备细胞裂解液作为初始物。对于初始物体积大于500ml的较大规模的制备,则需要考虑采用不同初始物的损益率。如果收集时大部分(90%以上)目标病毒仍存在于细胞中,为了减少操作大体积初始物带来的不便,可以考虑放弃上清中含有的目标病毒,而通过低速离心收集细胞,重悬于较小体积的缓冲液中进行细胞裂解制备细胞裂解液。对于腺病毒和AAV病毒的大规模制备都可以采用这种方式。如果通过延长培养时间可以使大部分的目标病毒释放到培养液中,也可以只收集培养上清而弃去细胞,从而省去反复冻融的步骤。除了用反复冻融方法裂解细胞外,还可以根据目标病毒的生物学性质采用其它不影响其感染性的方法,如超声法、化学法(如在AAV病毒制备中用脱氧胆酸或氯仿)等。初始物的浓缩当初始物体积较大时,要考虑对其进行浓缩后再分离纯化。浓缩时最好同时能获得初步纯化对于效果。 在不影响目标病毒的感染性的前提下,可选用盐析、超滤、透析等方法进行浓缩。盐析法不仅可以用来浓缩初始物,同时也能达到一定的分离纯化效果。最常用的盐是硫酸铵;许多病毒如腺病毒、AAV病毒和单纯疱疹病毒用聚乙二醇/氯化钠系统沉淀可获得很好的效果并且不影响病毒的感染性。目标病毒的分离纯化氯化铯密度梯度离心法至今仍是分离纯化各种病毒的最常用方法。主要是依据不同病毒具有特征性的浮力密度,使其与细胞裂解液中的其它成分分离开来。收集到目标病毒特异的组分后,用透析法除去其中的氯化铯,获得纯化的病毒。用这种方法有时可获得极高纯度的病毒。目前在临床实验中应用的重组腺病毒大都是用这种方法进行纯化的。然而,这种方法也存在明显的弊病,主要是费时且操作难度较大,重复性较差;并且氯化铯对人体有毒性。因此随着基因治疗研究和应用的发展,用这种方法纯化的重组病毒可能不能适应人体内应用的要求。用柱层析法尤其是亲和层析法分离纯化病毒可能成为用于临床的重组病毒载体大量纯化的主要方向。将病毒的特异性受体、抗体或化学合成的配体偶联在层析基质上并制备成亲和层析柱。将含有目标病毒的初始物经简单处理后直接上柱,最后洗脱和收集目标病毒。这类方法最近在AAV病毒载体的纯化中得到很高评价(Summerford C and Samulski J 1999)。返回标题第四节 病毒载体在基因治疗中的应用自1989年开展第一例人体基因转移(标记基因)以来,据不完全统计,至今已开展了近400项基因治疗临床试验,涉及病人1000余人。2/3以上的临床方案中应用了病毒载体进行基因导入。由于各种的病毒载体具有其特定的生物学特性,这些特性决定了其在基因治疗中的应用。表2列举了常用的病毒载体的特性和适用范围 载体 顺式作用元件 经典包装方式 反转录病毒载体 1) 两个长末端重复序列(LTR):含有整合和调节转录的必需序列;含有转录增强子和启动子;2) tRNA 引物结合位点p;3)包装信号序列ψ。 载体质粒转染整合了所有必需基因(gag,pol,env)的包装细胞系如PA317,经选择培养获得产病毒细胞系(VPC);细胞不裂解,病毒不断分泌至培养上清中。腺病毒载体 两个末端倒转重复序列(ITR);包装信号序列。 载体质粒与辅助质粒共转染293细胞获得重组腺病毒。重组腺病毒感染293细胞而扩增;细胞每次被感染后发生裂解。腺病毒伴随病毒载体 两个末端倒转重复序列(ITR);其中包括了病毒复制起点、包装信号及整合和拯救所必需的顺式元件。 AAV载体质粒和辅助质粒共转染293细胞,并加辅助病毒(腺病毒或单纯疱疹病毒)感染。单纯疱疹病毒载体 HSV 病毒复制起点ori; 病毒包装信号pac 。 PTCA重组病毒在互补细胞上传代;扩增子病毒在辅助病毒存在下传代。返回标题第五节 安全性检测总的来说,病毒载体制剂的安全性检测主要涉及以下几个方面(Aderson RW 1996):有复制能力的病毒(replication competent virus, RCV)RCV一般产生于重组病毒生产过程中基因重组事件。由于RCV具有复制能力,在生产过程中一旦出现,就可能呈现优势生长,从而影响载体病毒的产量;另外,含有RCV的制品用于人体内可能导致严重的病毒感染或急性/慢性病毒血症。RCV是对用于基因治疗的病毒载体制剂安全性检测的特有项目。检测RCV的方法因不同的病毒载体而异。对于反转录病毒载体,检测有复制能力的反转录病毒(RCR)的方法主要是PG4 S+L- 分析法;也可采用其它的变通方法。对于腺病毒载体,检测有复制能力的腺病毒(RCA)主要采用空斑分析方法及PCR方法。辅助病毒对于生产过程需要辅助病毒参与的病毒载体如AAV病毒载体,由于这些辅助病毒本身具有复制能力并对机体细胞有破坏性,因此检测病毒制剂中的辅助病毒如腺病毒或单纯疱疹病毒的残存量十分重要。其它成分的污染检测指标包括细菌、霉菌、支原体、内毒素等及在纯化过程中所用试剂如氯化铯等的残余量。返回标题第六节 病毒载体的发展方向基因治疗研究的快速发展对基因导入系统提出了越来越高的要求。病毒载体的发展也日新月异,包括对已有病毒载体的不断改进和完善,和越来越多的其它病毒被改造成为新的病毒载体。病毒载体的发展方向可归纳成以下几个方面:简便、高效的病毒载体包装系统:考虑到包装的高效性和操作的简便性,越来越多的病毒载体生产系统将采用双组成因素系统。这种系统容易用于病毒载体的大规模生产(Liu XL et al.1999; Conway JE et al. 1999 )。无病毒基因的病毒载体(gutless viral vector):去除病毒载体中所有的病毒基因,只保留其复制和包装所必需的顺式作用元件,是病毒载体的重要发展方向。这种策略可最大限度地扩大载体的容量和减少病毒对细胞的直接毒性和病毒蛋白表达引起的免疫毒性。 腺病毒的微载体(mini-Ad)、AAV载体、HSV扩增子载体都是无病毒基因的病毒载体。这一发展方向需要解决的主要问题是建立高效、安全(无辅助病毒污染)的包装系统。可调控表达外源基因的病毒载体:在许多疾病的基因治疗中,实现外源基因的可调控表达十分重要。如糖尿病的基因治疗,外源的胰岛素基因的表达最好能受血糖水平的调控;肾性贫血的基因治疗,EPO基因的表达最好能受血氧含量的调控等。目前所研究的表达调控系统主要包括各种药物诱导的表达"开关"。其中四环素控制系统(Tet-on和Tet-off)是人工设计的一种基因表达调控模式。这个系统在体外和动物体内实验中都表现出良好的表达调控作用(Fotaki ME et al.1997; Miller N and Whelan J 1997)。然而,由于其中的反式作用蛋白(tTA或rtTA)是异种蛋白,在机体内可能引起毒性作用和免疫反应,因此用于人体前还需考虑其安全性和长期有效性。最近Binley K等(1999)报道了用缺氧反应元件(hypoxia response element ,HRE)腺病毒载体中外源基因的表达。缺氧可激活bHLH/PAS 家族转录因子的表达,它们可结合HRE核心序列上,诱发其下游基因的表达。自我扩增型载体(self-amplifying vector)(Herweijer H and Wolff JA 1997):目前的基因转移方法基因转移的效率仍相对较低,尤其是在体内。为了提高外源基因表达总水平,除了提高基因转移效率外,另一种方法是尽量提高外源DNA在细胞中的表达水平。用自我扩增型的表达载体是达到此目的的方法之一。这些载体是以正链RNA病毒Sindbis病毒和Semliki Forest 病毒为基础的。用目的基因代替病毒的外壳蛋白编码序列,导入靶细胞中,病毒的复制蛋白大量复制重组的基因组,mRNA水平的大量增加导致高水平的转导基因表达。自我扩增型载体的表现形式可以是RNA,DNA和重组病毒。特异性复制型性病毒载体(specifically replicating vector):指可在某种特性的组织中产毒性复制,而在其它组织中不增殖的病毒载体。这类病毒载体主要用于特异性裂解肿瘤细胞。如腺病毒突变株Onyx-015是55Kdal E1B基因功能缺失的腺病毒突变株,可以选择性地在p53基因突变的肿瘤细胞中增殖,而在正常组织细胞中不增殖。用这种突变株联合化疗治疗恶性肿瘤II期临床结果令人鼓舞(Kirn D et al. 1998),已进入III期临床试验,从此,许多人工改造的腺病毒突变体被用于肿瘤治疗研究中。如将腺病毒E1基因的表达用甲胎蛋白(AFP)启动子控制,使得该病毒只能在甲胎蛋白阳性的肝癌细胞中增殖导致细胞死亡。也可以将这种病毒的基因组分开装在两个互补的缺陷性腺病毒载体上(Alemany R et al. 1999),其中一个载体除了腺病毒复制和包装所必需的顺式作用元件外,只含有E1区基因,其中E1A基因的表达受AFP启动子的控制;另一个载体为E1区缺失的重组腺病毒。这两个载体同时感染AFP阳性的肝癌细胞时,病毒可不断增殖而引起细胞死亡;对于AFP阴性的细胞,E1A基因不表达,因而病毒不能增殖。类似的设计在HSV载体系统也有报道。除了用甲胎蛋白作为反式激活因子外,各种其它组织特异性表达的蛋白和调控序列都可被用来控制病毒的增殖。嵌合型病毒载体(hybrid viral vector):是指将不同病毒的基因元件进行组合,形成的重组杂合病毒。如腺病毒与AAV病毒的杂合体病毒,既具有腺病毒的感染性和基因组特性(双链线状DNA),又具有AAV病毒的染色体整合性(Lieber A et al. 1999)。各种病毒基因元件组合形成新的杂合载体的报道层出不穷,如单纯疱疹病毒扩增子与AAV病毒杂合载体(Johnston KM et al. 1997)、腺病毒与EB病毒复制子杂合载体(Tan BT et al. 1999)腺病毒与反转录病毒杂合载体(Caplen NJ et al. 1999)等,不一而足。这些杂合载体使重组病毒的特性多样化,以适应不同基因转移目的的需要。靶向性病毒载体(targeted viral vector):是指能特异性地结合并感染某种细胞的病毒载体。靶向性病毒载体的产生主要有两种方法。一种方法是将病毒颗粒与某一靶向分子(Harari OA et al.1999 )或特异性抗体(Bartlett JS et al. 1999)偶联;另一种方法是通过改变病毒外壳蛋白的结构,使其对细胞的亲嗜性发生改变(Reynolds P et al. 1999;Krasnykh VN et al. 1996 )。参考文献Aderson RW. 1996. Forum"96: Gene Therapy. P21-24Alemany R, Lai S, Lou YC et al. 1999. Complementary adenoviral vectors for oncolysis. Krasnykh VN, Mikheeva GV. Douglas JT et al. 1996. Generation of recombinant adenovirus vectors with modified fibers for altering viral tropism. J Virol. 70:6839-6846.Lieber A, Steinwaerder.DS, Carlson CA et al. 1999. Integrating adenovirus-adeno-associated virus hybrid vectors devoid of all viral genes J Virol. 73:9314-9324Liu XL, Clark KR, Johnson PR. 1999. Production of recombinant adeno-associated virus vectors using a packaging cell line and a hybrid recombinant adenovirus. Gene Ther. 6:293-299Miller N and Whelan J. 1997. Progress in transcriptionally targeted and regulatable vectors for genetic therapy. Hum. Gene Ther. 8:803-815Pyles RB, Warnick RE, Chalk CL et al.1997 A novel multiply-mutated HSV-1 strain for the treatment of human brain tumors. Hum. Gene Ther. 8:533-544Reynolds P, Dmitriev I, Curiel D. 1999. Insertion of an RGD motif into the HI loop of adenovirus fiber protein alters the distribution of transgene expression of the systemically administered vector.Rolling F and Samulski J. 1995 AAV as a viral vector for human gene therapy. Molecular Biotechnology. 3:9-15Summerford C and Samulski J. 1999 Viral receptors and vector purification : new approaches for generating clinical-grade reagents. Nature Med. 5:587-588伍志坚,吴小兵,侯云德. 1999. 具有AAV载体包装功能的重组HSV的产生. 科学通报. 44(5):506-509吴小兵,董小岩,伍志坚等. 1998. 以粘粒为基础产生重组单纯疱疹病毒HSV1-lacZ的研究. 病毒学报. 14:359-364

this agreement will become effective as of the date hereof dnd

dnd是died a natural death的简写,自然死亡。 全句意为:本协议从自然死亡之日起即可生效。 楼主事先不说清楚,我怎么知道这句话不是出自遗产协议书!?以后把脑袋洗干净了再来提问。

有一首节奏感很强英文歌有一句歌词是come on 摔,糊呃river,适合做跳舞做热身运动的,叫什

Purple Lamborghini (Explicit) (《X特遣队》电影插曲) - Skrillex/Rick RossWritten by:Rick RossBiggest boss and I been the trillestI"m a bigger problem when I click with SkrillexMurder on my mind it"s time to pray to godMy revolver is that religion"s the revolution"s bondYou wanna know my name then go and tell them SargYou wanna know my gang suicide squadPistol on my waist I might make a mistakeDead shot head shot oh my god am I crazyDrugs every corner this is Gotham cityKiller Croc came to kidnap you to cut out your kidneyAin"t no mercy got that purple Lamborghini lurkin"Rozay don"t you know that pussy worth itFlooded Rolex at the Grammy awardsThey still sellin" dope that"s those Miami boysKillers everywhere it ain"t no place to runForgive me for my wrongs I have just begunAin"t no mercy a ain"t no mercyGot that purple Lamborghini purple Lamborghini lurkin"Ain"t no mercy a ain"t no mercyGot that purple Lamborghini lurkingDon"t be beggin" for your life cause that"s a lost causeHigh stakes body armor suicide boyThere"s a time for games and there"s a time to killMake up your mind baby there"s no time to chillCapital murder capital lettuceYeah she catchin" my vibe but can"t fathom my cheddarNeed a couple gang members for these new endeavorsFrom this point on whenever we do we do togetherBody on the corner million in the trunkSeven figures I will spend that every other monthKillers on the corner talons in the clipVilla Palisade and Paris just to fill with bitchesSay my name and I"m coming with the gun squadEverybody runnin" homie there"s only one godCocaine white Ferrari I"m in the fast laneEvery day was life and death that"s when the cash cameCount money drug residue even blood on "emHe had a driver "til I put my cause on itKicking in the dope boy suicide squadNeedle in my arm so I"m do or die for itAin"t no mercy a ain"t no mercyGot that purple Lamborghini purple Lamborghini lurkin"Ain"t no mercy a ain"t no mercyGot that purple Lamborghini lurkin" RozayPrideGreedDeviousNeedsHighstakesCash moneyDope boyRap moneyReal hipForgive me for my wrongs I have just begunRozay

come α gaⅰn αn other day是什么意思?

另外那天再来吧!

effective date , commencement date ,start date 分别是什么日期?

effective date 生效日期commencement date动工日期;施工日期;生效日期;开工日start date 开始日期

use only under competent supervision什么意思

use only under competent supervision的中文翻译use only under competent supervision 只能在主管的监督下使用

求翼年代记里的ship of fools 如题,有的话请发到邮箱1586725279@qq.com

不是这个歌…………

take place ,happen, occur, come about, break out这些单词用法有什么区别

(1). take place 表示“发生、举行、举办”,一般指非偶然性事件的“发生”,即这种事件的发生一定有某种原因或事先的安排,例如:Great changes have taken place in our hometown during the past ten years.The Olympic Games of 2008 will take place in Beijing.(2) .happen作“发生、碰巧”解,一般用于偶然或突发性事件,例如:What happened to you? (一般不说:What did you happen?)Maybe something unexpected happened.I happened to see him on my way home.= It happened that I saw him on my way home.(3). occur作“发生、想到、突然想起”解,其意义相当于happen,例如:What has occurred? (=What has happened?)A big earthquake occurred (=happened) in the south of China last month.It occurred to me that she didn"t know I had moved into the new house.(4). come about表示“发生、产生”,多指事情已经发生了,但还不知道为什么,常用于疑问句和否定句,例如:When Mother woke up, she didn"t know what had come about.I"ll never understand how it came about that you were late three times a week.Do you know how the air accident came about?(5). break out意思为“发生、爆发”,常指战争、灾难、疾病或者争吵等事件的发生,也可以表示突然大声叫喊等,例如:Two world wars broke out last century.A fire broke out in the hospital in the mid-night.After the flood, diseases broke out here and there.She broke out, “That is too unfair!”

求listen to the stereo(家庭教师)钢琴谱 要全的 发1043546426@qq.com

come from california是哪歌的歌词?

加州旅馆

求家庭教师所有角色歌打包下载 MP3式 357917504@qq.com

  片头曲  《家庭教师ヒットマンREBORN! OP1-Drawing days/SPLAY》(1~26)  《家庭教师ヒットマンREBORN! OP2-BOYS & GIRLS/LM.C》(27~51)  《家庭教师ヒットマンREBORN! OP3-DIVE TO WORLD/CHERRYBLOSSOM》(52~73)  《家庭教师ヒットマンREBORN! OP4-88/LM.C》(74~101)  《家庭教师ヒットマンREBORN! OP5-last cross/光冈昌美》(102~126)  《家庭教师ヒットマンREBORN! OP6-EASY GO/加藤和树》(127~153)  《家庭教师ヒットマンREBORN! OP7-Funny Sunny Day/SxOxU》(154~177)  《家庭教师ヒットマンREBORN! OP8-LISTEN TO THE STEREO!!/GOING UNDER GROUND》(178~203)  片尾曲  《家庭教师ヒットマンREBORN! ED1-道标/橘庆太》(1~12)  《家庭教师ヒットマンREBORN! ED2-ONE NIGHT STAR/the ARROWS》(13~29)  《家庭教师ヒットマンREBORN! ED3-Echo again/SPLAY》(30~38)  《家庭教师ヒットマンREBORN! ED4-friend/アイドリング!!!》(39~51)  《家庭教师ヒットマンREBORN! ED5-Sakura addiction/云雀恭弥VS六道骸》(52~62)  《家庭教师ヒットマンREBORN! ED6-friend/笹川京子VS三浦ハル》(63)  《家庭教师ヒットマンREBORN! ED7-STAND UP!/Lead》(64~76)  《家庭教师ヒットマンREBORN! ED8-アメあと/w-inds.》(77~89)  《家庭教师ヒットマンREBORN! ED9-CYCLE/CHERRYBLOSSOM》(90~101)  《家庭教师ヒットマンREBORN! ED10-すべり台/森翼》(102~114)  《家庭教师ヒットマンREBORN! ED11-桜ロック/CHERRYBLOSSOM》(115~126)  《家庭教师ヒットマンREBORN! ED12-Smile for.../上户彩》(127~139)  《家庭教师ヒットマンREBORN! ED13-青い梦/森翼》(140~153)  《家庭教师ヒットマンREBORN! ED14-梦のマニュアル/CHERRYBLOSSOM》(154~165)  《家庭教师ヒットマンREBORN! ED15-gr8 story/SuG》(166~177)  《家庭教师ヒットマンREBORN! ED16-ファミリア/D-51》(178~190)  《家庭教师ヒットマンREBORN! ED17-キャンバス/+Plus》(191~203)  插曲  OST专辑:  《家庭教师ヒットマンREBORN! オリジナル サウンドトラック~标的1~》  《家庭教师ヒットマンREBORN! オリジナル サウンドトラック~标的2~》  《家庭教师ヒットマンREBORN! オリジナル サウンドトラック~标的3~》  《家庭教师ヒットマンREBORN! オリジナル サウンドトラック~标的4~》(已发售)
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