- meira
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Objective: This study and understand the past, untreated mung bean shells, untreated mung bean shells, shell-rich GABA deal with mung bean, mung bean processing GABA-rich shell, Se treatment Peeled mung bean, mung bean shell Se handle six substances in the amount of total flavonoids The optimal extraction conditions, the optimal concentration measurements as well as the extraction of antioxidant flavonoids and vitamin C, antioxidant comparison.
Methods: The sample extraction method of total flavonoids, including water bath water bath reflux extraction and ultrasonic extraction method, the sample determination of total flavonoids, including aluminum nitrate chromogenic method and direct color method, the sample determination of antioxidant flavonoids, including FRAP method and DPPH method.
Results: The reflux extraction method of extracting the raw mung bean shell, rich green bean shell GABA treatment, Se treatment mung bean shell, raw peeled mung beans, rich GABA treatment peeled mung beans, mung bean shell Se treatment the amount of total flavonoids six substances (mg / g), respectively 35.3,37.98,45.16,1.12,1.46,3.38.
Ultrasonic extraction of untreated mung bean shell extract, rich green bean shell GABA treatment, Se treatment mung bean shell, raw peeled mung beans, rich GABA treatment peeled mung beans, mung bean shelling Se treatment the amount of total flavonoids of six substances ( mg / g), respectively 43.9,43.0,51.68,2.36,1.82,3.12.
FRAP method measured antioxidant capacity of vitamin C (as FeSO4 equivalents μg / μg) of 4.14, reflux extraction method extracted to the untreated mung bean shells, rich green bean shell GABA treatment, selenium-rich bean shell three kinds of material dealing with anti-oxidant capacities of 0.73,0.69,0.80, three peeled mung bean products are not detected because of the content extraction. Extracted by ultrasonic extraction of non-deal with the past shell bean, untreated mung bean shell, shell-rich GABA deal with mung bean, mung bean processing GABA-rich shell, Se treatment Peeled mung bean, mung bean shell six kinds of material dealing with selenium-rich antioxidant capacity 0.40,0.79,0.66,0.77,0, respectively.
71,0.87.
DPPH law of vitamin C, free radical scavenging rate was 95.44%, reflux extraction of the extracted raw mung bean shells in the past, untreated mung bean shells, shell-rich GABA deal with mung bean, mung bean processing GABA-rich shell, Se-treatment to Shell mung bean, mung bean shell Se handle six substances radical scavenging rates were 9.26%, 89.81%, 21.52%, 81.71%, 26.62%, 79.86%.
Extracted by ultrasonic extraction method to the untreated mung bean shells in the past, untreated mung bean shells, shell-rich GABA deal with mung bean, mung bean processing GABA-rich shell, Se treatment Peeled mung bean, mung bean shell Se handle six substances scavenging rates were 78.9%, 91.13%, 64.27%, 91.13%, 61.87%, 89.69%.
Conclusion: The optimal extraction method for the ultrasonic extraction method; ultrasonic water bath extraction of the optimum extraction condition is the concentration of ethanol was 30%, solid-liquid ratio of 1:60, water bath temperature of 70 ℃, ultrasonic power is 200 watts, extract time is 30 minutes; of total flavonoids extract the best method for determination of aluminum nitrate chromogenic method; have the antioxidant capacity of the samples and both arrived on the antioxidant capacity of vitamin C, mung bean shells of total antioxidant capacity of flavonoids Peeled mung bean class than those in the total antioxidant capacity of flavonoids
- 康康map
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Objective: this study did not handle through understanding of past shell mung bean, not processed gram carapace, rich GABA processing shell, mung beans, GABA processing se-enriched processing shell mung bean, selenium processing gram carapace six material flavonoids of optimal extraction conditions, the optimal measurement methods and content of flavonoids extraction of oxidation resistance and vitamin C oxidation resistance.
Methods: the method for extracting flavonoids samples including thermostatic water refluxing extraction and thermostatic water by ultrasonic wave extraction, sample the flavonoids content determination methods including 9h2o colour-display method and direct method, sample flavonoids oxidation resistance measurement method including FRAP and DPPH.
Results: the refluxing extraction extraction to the pending gram carapace, rich GABA processing gram carapace, selenium, treatment with gram carapace shell, mung beans, GABA processing shell selenium-rich shell with six kinds of material flavonoids mung bean (mg/g) respectively, 37.98 35.3 45.16, and 1.46, 1.12, 3.38.
Ultrasonic extraction method to extract processed gram carapace, rich GABA processing gram carapace, selenium, treatment with gram carapace shell, mung beans, GABA processing shell selenium-rich shell with six kinds of material flavonoids mung bean (mg/g) respectively, and 51.68 43.9 43.0 2.36, 1.82, 3.12,.
FRAP method that vitamin C antioxidant capacity (FeSO4 equivalent plan muon g/mu g) for dope-containing refluxing extraction, the extraction through gram carapace, rich GABA processing gram carapace, selenium processing gram carapace three material antioxidant capacity, respectively, 0.80 1-year 0.69, three shelled mung bean products are not testing for extraction. Ultrasonic extraction method to extract the processing of the past, not processed shell mung beans, rich GABA processing shell, mung beans, GABA processing se-enriched processing shell mung bean, selenium processing gram carapace six substance antioxidant capacity, respectively, 0.66, lingnan 0.40 0.77, 0.71, 0.87.
By DPPH method of vitamin C for 95.44% radicals clearance, refluxing extraction by the pending past shell mung bean, not processed gram carapace, rich GABA processing shell, mung beans, GABA processing se-enriched processing shell mung bean, selenium processing gram carapace six substance 9.26% respectively, the radicals clearance 21.52%, 81.71%, meso-radicantia and 26.62%, 79.86%. Ultrasonic extraction method to extract the shell with past gram, not processed gram carapace, rich GABA processing shell, mung beans, GABA processing se-enriched processing shell mung bean, selenium processing gram carapace six material free radicals, respectively 91.13% clearance was 64.27%, 91.13%, 61.87%, 89.69%,.
Conclusion: the best extraction method for ultrasonic extraction, Ultrasonic thermostatic water extraction of the best extraction process condition is ethanol concentration ratio for 30%, 1 0, stretching temperature for 70 degrees, ultrasonic power for 200 watts, extracting time for 30 minutes, The flavonoids content in extract optimum determination method for aluminum nitrate color, The samples have antioxidant capacity and worth in vitamin C antioxidant capacity, gram carapace of flavonoids antioxidant capacity than green beans in the shell of antioxidant flavonoids