- 慧慧
-
外周血单个核细胞分离肝素正常人体血液密度梯度离心使用ficoll-paque(膜/医疗生物科学),根据制造商的指示。签署获得知情同意使用的每个人的血液样本机构审查委员会批准的协议。小鼠脾细胞进行孤立的切碎的脾脏,和治疗(30分钟,室温)与liberase blendzymes和抗酶(罗氏应用科学;后制造商的指示),其次是乙二胺四乙酸(10毫米,5分钟,房间温度sigma-aldrich)。光密度细胞进行分离,离心后在碘海醇介质(准确的化学和科学)。
- 牛云
-
人PBMC肝素正常人体血液中分离出
密度梯度离心法使用Ficoll分离Paque(Amersham公司/ GE医疗集团
生物科学),根据制造商的指示。签名
获得知情同意下利用每个人的血液样本
机构审查委员会批准的协议。小鼠脾细胞
分离切碎脾脏,治疗(30分钟,室温)
liberase blendzymes和DNA酶I(罗氏应用科学;以下
制造商的指示),用EDTA(10毫米,5分钟,房间
温度; Sigma-Aldrich公司)。光密度细胞分离后离心
在Nycodenz介质(精确化学与科学)。
- 陶小凡
-
Human PBMCs were isolated from heparinized normal human blood by
density gradient centrifugation using Ficoll-Paque (Amersham/GE Healthcare
Bio-Sciences), according to the manufacturer"s instructions. Signed
informed consent was obtained for use of each human blood sample under
an Institutional Review Board-approved protocol. Mouse splenocytes were
isolated by mincing the spleens, and treating (30 min, room temperature)
with liberase blendzymes and DNase I (Roche Applied Science; following
manufacturer"s instructions), followed by EDTA (10 mM, 5 min, room
temperature; Sigma-Aldrich). Light density cells were isolated after centrifugation
in Nycodenz medium (Accurate Chemical & Scientific).