阿霉素用Hoechst 33258 染色后用激光共聚焦显微镜照相,染料和阿霉素会产生同样颜色荧光如何区分

绿色激光（535 nm左右）激发下,（1）阿霉素发射红色荧光,峰值在620 nm左右；（2）PI发射红色荧光,峰值在615 nm左右.两个发射光是重叠的,因此不能同时使用.
在蓝色激光（488 nm左右）激发下,（1）阿霉素发射荧光峰值在575 nm左右；（2）PI发射荧光峰值在515 nm左右,两者发射光有一定差距,但仍属于比较近的,做判断还是挺费劲的.不过可以试一试.
你查过相关的文献吗?在测阿霉素对细胞凋亡的影响时只能用PI检测吗?实在不行就换别的荧光探针检测

This paper mainly studies the preparation encapsulation efficiency in more than 80% of hydrochloric acid doxorubicin liposomes injection,and has certain stability.This injection by doxorubicin or hydrochloric acid doxorubicin,neutral lecithin phospholipid and negative and cholesterol,antioxidants,organic acid,alkali,slow granule,injection water,this preparation techniques including blank homogenization liposome,liposome,preparation doxorubicin or hydrochloric acid doxorubicin liposomes and its suspension liquid,the capacity,aseptic,repacking,steps and preservation of liposome of hydrochloric acid doxorubicin for the determination of encapsulation efficiency.This injection good stability,encapsulation efficiency high,low cost,fewer side effects,the preparation technology is simple.
This paper studies using pH gradient legal prepare liposomes adriamycin.Through the nano technology,made by adjusting after nanometer sac capsule inside and outside the concentration of the ions pH value,loading hydrochloric acid doxorubicin,make doxorubicin outside into the nano from sac,and sac within the capsule total adriamycin,the proportion of adriamycin lower than 20%