ll

多于一个就加，就一个不加。

拉下来的

这里意思是接下来一周，这个本来就是直接接的，前面的日期也是必须在前面要加介词on的，有时英语里它就是不用接，你只要记住就行了，只是英语习惯！希望能帮到你！

不用

打开你们的练习册然后放下，老师说

选B is第二个问题，好像是就近原则。

the 加形容词可以代指某一类事物，这里应该也是这个用法，所以following是形容词，而不是名词，故不用加s

following什么意思下列的。following英 /u02c8fu0252lu0259u028au026au014b，美 /[u02c8fɑu02d0lou028au026au014b]adj.下列的;(时间上)接着的;下述的n.下列;如下;(统称)拥护者，追随者;下述prep.在(某事)以后;由于v.跟随;跟着;(指时间或顺序)在…后发生，因…而发生;在…后做follow的现在分词复数： followings牛津词典adj.the following…1（时间上）接着的next in timethe following afternoon/month/year/week第二天下午；第二个月；第二年；第二周They arrived on Monday evening and we got there the following day.他们是星期一晚上到的，我们次日也抵达那里。2下述的；下列的that is/are going to be mentioned nextAnswer the following questions.回答下列问题。

take the advice as follows，as follows 只能跟在后面take the following advice following 放在名词前面

可以,following有名词意思 下面披露很多项,就是followings

因为这个的意思是:其中的一本书.说明你的书肯定不止一本,所以要用复数.如果只有一本书,则应该写作:abookofmine

following这里考查非谓语动词，现在分词作前置定语修饰Sunday，意思是“接下来的星期天”希望对你有帮助，不懂可以再提出，满意请采纳~

the following 相当于一个指代词，因此句子中的谓语必须与实际的主语（这时在句中是在宾语位置）相一致

消逝的光芒thefollowing是信徒的。因为Techland旗下大受好评的游戏作品消逝的光芒最新资料片信徒预告发布，thefollowing就是信徒的意思，在简短的影像中主角在断崖间一路狂奔躲避丧尸追赶，不幸的是跳入水间依旧是丧尸活动之地，所以消逝的光芒thefollowing是信徒的。消逝的光芒信徒包括众多战役任务和支线任务，亦可自定义沙滩车。消逝的光芒信徒将于2016年的头三个月之内发售。消逝的光芒的游戏设定在游戏中，游戏主角Kyle Crane在一个受伤男人的身上发现了一幅神秘的地图，根据地图的说明，在哈兰城外有一批神秘的人群对僵尸病毒免疫，因此他将根据这幅地图的指引探索一片未知的地区。

is the tips

thefollowing相当于一个指代词，因此句子中的谓语必须与实际的主语（这时在句中是在宾语位置）相一致再看看别人怎么说的。

前者特指 后者泛指

the following 应该是做前置定语的,来修饰名词,所以得看名词的数,来决定谓语的数.following用作形容词表示“接着的”，与定冠词the连用，表示“下述的”。the following作主语时,谓语动词可用单数也可用复数。 扩展资料 　　例句： 　　The following is a summary of our conclusions. 　　现将我们的`几点结论综述如下。 　　Please mail us at the following email address. 　　请按下面的电邮地址发送给我们。

通常say+内容，tell sb. sth. tell sth. to sbspeak +语言（如English)

歌曲名:Game Of All Fours歌手:kate rusby专辑:The Girl Who Couldn"t FlyAs I was walking one midsummer"s morning,To hear the birds whistle and the nightingale play,Was there that I met a beautiful maiden,As I was a walking along the highway.Oh where are you going my fair pretty lady,Where are you going so early this morn,She answered, kind sir to visit my neighbours,I"m going down to Lincoln the place I was born.Oh may I go with you my fair pretty lady,May I go along in your sweet company,She turned her head round and smiling all at me,Said you may come with me kind sir if you please.We hadn"t been walking a few miles together,Before this young damsel began to show free,She sat herself down saying sit down beside me,The game we shall play will be one, two and three.I said my dear lady if you"re fond of the gaming,There"s one game I know I would like you to learn,The game it is called The Game Of All Fours,So I took out my pack and began the first turn.She cut the cards and I fell a dealing,I dealt her a trump and myself the poor Jack,She led off here ace and stole the Jack from me,Saying Jack is the card I like best in your pack.I dealt the last time, its your turn to shuffle,My turn to show the best card in the pack,Once more she"d the ace and stole the Jack from me,Once more I lost when I laid down poor Jack.So I took up my hat and I bid her good morning,I said you"re the best that I know at this game,She answered, young man, come back tomorrow,We"ll play the game over and over and over and over and over againhttp://music.baidu.com/song/15731201

kill the game杀死游戏

打成Jar包，直接用spark-submit 方式运行就可以成功，直接用IntelliJ就总是失败，不知道怎么回事，求大神指点。程序是object HelloSpark {def main(args:Array[String]): Unit ={if(args.length!=2){System.err.println("Usage:HelloSpark <Input> <Output>")System.exit(1)}val conf = new SparkConf().setAppName("helloSpark").setMaster("spark://master:7077").set("spark.executor.memory", "1g").set("spark.serializer", "org.apache.spark.serializer.KryoSerializer")val sc = new SparkContext(conf)sc.textFile(args(0)).map(_.split(" ")).filter(_.length==6).map(x=>(x(1),1)).reduceByKey(_+_).map(x=>(x._2,x._1)).sortByKey(false).map(x=>(x._2,x._1)).saveAsTextFile(args(1))sc.stop()}这是网上的一个实例，步骤都是一步一步来的。16/02/23 16:39:53 WARN TaskSetManager: Lost task 0.0 in stage 0.0 (TID 0, worker1): java.lang.ClassNotFoundException: com.spark.firstApp.HelloSpark$$anonfun$2at java.net.URLClassLoader.findClass(URLClassLoader.java:381)at java.lang.ClassLoader.loadClass(ClassLoader.java:424)at java.lang.ClassLoader.loadClass(ClassLoader.java:357)at java.lang.Class.forName0(Native Method)at java.lang.Class.forName(Class.java:348)at org.apache.spark.serializer.JavaDeserializationStream$$anon$1.resolveClass(JavaSerializer.scala:68)at java.io.ObjectInputStream.readNonProxyDesc(ObjectInputStream.java:1613)at java.io.ObjectInputStream.readClassDesc(ObjectInputStream.java:1518)at java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1774)at java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1351)at java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1993)at java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1918)at java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1801)at java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1351)at java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1993)at java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1918)at java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1801)......at org.apache.spark.executor.Executor$TaskRunner.run(Executor.scala:213)at java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1142)at java.util.concurrent.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:617)at java.lang.Thread.run(Thread.java:745)16/02/23 16:39:53 INFO TaskSetManager: Starting task 0.1 in stage 0.0 (TID 2, worker0, partition 0,NODE_LOCAL, 2134 bytes)16/02/23 16:39:53 INFO TaskSetManager: Lost task 1.0 in stage 0.0 (TID 1) on executor worker2: java.lang.ClassNotFoundException (com.spark.firstApp.HelloSpark$$anonfun$2) [duplicate 1]16/02/23 16:39:53 INFO TaskSetManager: Starting task 1.1 in stage 0.0 (TID 3, worker2, partition 1,NODE_LOCAL, 2134 bytes)16/02/23 16:39:53 INFO TaskSetManager: Lost task 1.1 in stage 0.0 (TID 3) on executor worker2: java.lang.ClassNotFoundException (com.spark.firstApp.HelloSpark$$anonfun$2) [duplicate 2]16/02/23 16:39:53 INFO TaskSetManager: Starting task 1.2 in stage 0.0 (TID 4, worker1, partition 1,NODE_LOCAL, 2134 bytes)16/02/23 16:39:53 INFO TaskSetManager: Lost task 1.2 in stage 0.0 (TID 4) on executor worker1: java.lang.ClassNotFoundException (com.spark.firstApp.HelloSpark$$anonfun$2) [duplicate 3]16/02/23 16:39:53 INFO TaskSetManager: Starting task 1.3 in stage 0.0 (TID 5, worker1, partition 1,NODE_LOCAL, 2134 bytes)16/02/23 16:39:53 INFO TaskSetManager: Lost task 1.3 in stage 0.0 (TID 5) on executor worker1: java.lang.ClassNotFoundException (com.spark.firstApp.HelloSpark$$anonfun$2) [duplicate 4]16/02/23 16:39:53 ERROR TaskSetManager: Task 1 in stage 0.0 failed 4 times; aborting job16/02/23 16:39:53 INFO TaskSchedulerImpl: Cancelling stage 016/02/23 16:39:53 INFO TaskSchedulerImpl: Stage 0 was cancelled16/02/23 16:39:53 INFO DAGScheduler: ShuffleMapStage 0 (map at HelloSpark.scala:20) failed in 10.015 s16/02/23 16:39:53 INFO DAGScheduler: Job 0 failed: sortByKey at HelloSpark.scala:21, took 10.156984 sException in thread "main" org.apache.spark.SparkException: Job aborted due to stage failure: Task 1 in stage 0.0 failed 4 times, most recent failure: Lost task 1.3 in stage 0.0 (TID 5, worker1): java.lang.ClassNotFoundException: com.spark.firstApp.HelloSpark$$anonfun$2at java.net.URLClassLoader.findClass(URLClassLoader.java:381)at java.lang.ClassLoader.loadClass(ClassLoader.java:424)at java.lang.ClassLoader.loadClass(ClassLoader.java:357)at java.lang.Class.forName0(Native Method)at java.lang.Class.forName(Class.java:348)at org.apache.spark.serializer.JavaDeserializationStream$$anon$1.resolveClass(JavaSerializer.scala:68)at java.io.ObjectInputStream.readNonProxyDesc(ObjectInputStream.java:1613)at java.io.ObjectInputStream.readClassDesc(ObjectInputStream.java:1518)at java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1774)at java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1351)at java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1993)at java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1918)at java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1801)at java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1351)at java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1993)at java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1918)at java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1801)at java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1351)at java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1993)at java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1918)at java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1801)at java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1351)at java.io.ObjectInputStream.readObject(ObjectInputStream.java:371)at scala.collection.immutable.$colon$colon.readObject(List.scala:362)at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:62)at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43)at java.lang.reflect.Method.invoke(Method.java:497)at java.io.ObjectStreamClass.invokeReadObject(ObjectStreamClass.java:1017)at java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1896)at java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1801)at java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1351)at java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1993)at java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1918)at java.io.ObjectInputStream.readOrdinaryObject(ObjectInputStream.java:1801)at java.io.ObjectInputStream.readObject0(ObjectInputStream.java:1351)at java.io.ObjectInputStream.defaultReadFields(ObjectInputStream.java:1993)at java.io.ObjectInputStream.readSerialData(ObjectInputStream.java:1918)......at org.apache.spark.serializer.JavaSerializerInstance.deserialize(JavaSerializer.scala:115)at org.apache.spark.scheduler.ShuffleMapTask.runTask(ShuffleMapTask.scala:64)at org.apache.spark.scheduler.ShuffleMapTask.runTask(ShuffleMapTask.scala:41)at org.apache.spark.scheduler.Task.run(Task.scala:89)at org.apache.spark.executor.Executor$TaskRunner.run(Executor.scala:213)at java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1142)at java.util.concurrent.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:617)at java.lang.Thread.run(Thread.java:745)Driver stacktrace:at org.apache.spark.scheduler.DAGScheduler.org$apache$spark$scheduler$DAGScheduler$$failJobAndIndependentStages(DAGScheduler.scala:1431)at org.apache.spark.scheduler.DAGScheduler$$anonfun$abortStage$1.apply(DAGScheduler.scala:1419)at org.apache.spark.scheduler.DAGScheduler$$anonfun$abortStage$1.apply(DAGScheduler.scala:1418)at scala.collection.mutable.ResizableArray$class.foreach(ResizableArray.scala:59)at scala.collection.mutable.ArrayBuffer.foreach(ArrayBuffer.scala:47)at org.apache.spark.scheduler.DAGScheduler.abortStage(DAGScheduler.scala:1418)at org.apache.spark.scheduler.DAGScheduler$$anonfun$handleTaskSetFailed$1.apply(DAGScheduler.scala:799)at org.apache.spark.scheduler.DAGScheduler$$anonfun$handleTaskSetFailed$1.apply(DAGScheduler.scala:799)at scala.Option.foreach(Option.scala:236)at org.apache.spark.scheduler.DAGScheduler.handleTaskSetFailed(DAGScheduler.scala:799)at org.apache.spark.scheduler.DAGSchedulerEventProcessLoop.doOnReceive(DAGScheduler.scala:1640)......Caused by: java.lang.ClassNotFoundException: com.spark.firstApp.HelloSpark$$anonfun$2at java.net.URLClassLoader.findClass(URLClassLoader.java:381)at java.lang.ClassLoader.loadClass(ClassLoader.java:424)at java.lang.ClassLoader.loadClass(ClassLoader.java:357)at java.lang.Class.forName0(Native Method)at java.lang.Class.forName(Class.java:348)at org.apache.spark.serializer.JavaDeserializationStream$$anon$1.resolveClass(JavaSerializer.scala:68)at java.io.ObjectInputStream.readNonProxyDesc(ObjectInputStream.java:1613)at java.io.ObjectInputStream.readClassDesc(ObjectInputStream.java:1518)......16/02/23 16:39:53 INFO SparkContext: Invoking stop() from shutdown hook16/02/23 16:39:53 INFO SparkUI: Stopped Spark web UI at :404016/02/23 16:39:53 INFO SparkDeploySchedulerBackend: Shutting down all executors......Process finished with exit code 1这个问题我解决了，在设置SparkConf()的时候，需要把本机生成的JAR包路径进行制定，如：val conf = new SparkConf().setAppName("SogouResult").setMaster("spark://master:7077").setJars(List("D:\IDEA workspace\helloSpark\out\artifacts\helloSpark_jar\helloSpark.jar"))

我也进不去，是不是被封了？

美国往事》包含了一个男人在这个世界上所能遇到的一切。友情、爱情、想、责任、冲突。它更像是一场让人不愿醒来的梦，当面条躺在床上，在温暖的灯光和的迷离的电话铃声中回到那些逝去了的岁月，这场梦便开始了，

www.dy106.com

June 10th Sunday Fine 　　 Yesterday was the ninth day of the World Cup. There was a football match between China and Brazil. We know Brazilian Team is the top one of the world. Though the players of the Chinese Team tried their best, they still lost the game. The result of the game was 4:0. I think they did better than the first game.I believe they can learn a lot from Brazilian Team. I hope the team of China will be a strong team one day. 6月10日 星期日 睛 昨天是世界杯赛的第九天。中国队对巴西队。我们都知道巴西队是世界上的足球队。虽然中国队队员竭尽了全力，但是还是输了。比赛结果四比零。我觉得中国队表现比第一场要好多了。我相信，他们能从巴西队那里学到很多东西。 我希望中国足球队终有一天成为强队。 June 10th Sunday Fine Yesterday evening the team of China played against the team of Brazil. The game started at 7: 30. It was an exciting game.Brazilian Team is the best one in the world. Everyone in the team of China tried his best, but the team of Brazil was too strong.Chinese Team couldn"t stop them. They kicked four goals, and our team lost the game. We hope the players in Chinese Team can make great progress, and it will be a strong team one day. 6月10日 星期日 睛 昨天晚上，中国足球对迎战巴西足球队。比赛在7：30开始。那是一场令人激动的比赛。巴西队是世界上的足球队，中国队的每个队员都尽了努力，但是巴西队太强大了。中国队根本抵御不住他们的进攻，最终以零比四输了这场比赛。 我们希望中国足球队队员能取得较大的进步，将来能成为一个强队。

他是第一季的FBI ，刚出场的时候是坏人 ，负责抓迈克他们，受雇于公司 ，是女总统的亲信，后来被总统抛弃，就帮助迈克了，最后出庭指控总统 救了SARA ，第一季最后疑似被暗杀，实际没死

少男杀手

pubmed收录了>2000万的论文摘要，PMC收录了>100万的全文。所以你要下的话，最好找个大学的ip。这个我最有研究了！

pubmed上显示ecollection的方法是：1、在浏览器中检索pubmed官网链接。2、在打开的pubmed网站中搜索关键词，可以搜中文，也可以搜索英文，点击搜索。3、搜索成功后，网站会展示搜索的结果，在每个链接上面，会自动显示文章的影响因子，同时点击右侧的中文/英文按钮，可以自动翻译题目和摘要。4、按影响因子筛选，可以点击搜索框中的方格，打开高级搜索菜单，选择影响因子的范围即可。

这则讯息及其附件不为公开并可能享有法律特权。任何未经授权的报道、使用或传播是严行禁止的。如果你不是预定收受人，请立即告知发送人彻底删除讯息及其附件，并销毁复件。非常感谢您的合作！

合法的特权

英语否定句没则怎么改1、有系动词be（am,is,are,was,were）的,在后面加not;2、有情态动词can,may,must等的，在后面加not;3、已经有了助动词的，在后面加not;4、没有前面三种情况的，要用到助动词do,does,did加not，放在动词原形前面；5、特轿枯别情闭察洞况，有些句子本身有不定代词做主语或者宾语了的，可以把不定代词改为否定就可以。《sport.ecios.cn/article/546893.html》《sport.hzzyqz.cn/article/374820.html》《sport.xcuvrqn.cn/article/047358.html》《sport.onegrass.cn/article/825397.html》《sport.cskylog.cn/article/524187.html》《sport.93159.cn/article/710684.html》

E色 VS1 3EX 无荧光无奶咖 定制镶嵌 11000能做好，金重3.5-4克 ，无伴钻镶，秒杀你这颗裸石！

blue中的e发音与后两者不同。

当然是A啦

根据具体语境才能准确判断，一般不用

It is generally considered impolite to take a mobile phone call during a business dinner, as this can disrupt the flow of the dinner and suggest that the caller is more important than the person they are meeting with. However, there may be some exceptions to this rule. For example, it may be appropriate to take a call if it is urgent and cannot be postponed until after the dinner. In this situation, it is best to inform the person you are meeting with ahead of time and apologize for any disruption the call may cause. You can also ask for permission to take the call before doing so.Another exception may be if you are expecting an important call related to the business or deal being discussed at the dinner. In this case, it is best to inform the other party at the start of the dinner that you are expecting a call, and politely excuse yourself to take the call when it comes. It is important to keep the call brief and return to the dinner as soon as possible.Overall, taking a mobile phone call during a business dinner should be avoided whenever possible, as it is often seen as disrespectful to the person you are meeting with. Exceptions should be made sparingly and handled with care.

不一样，blue 不发音red 发欸(音标打不出来)yellow发E本身的音

【答案】A【解析】选项A的音标是[bluː]；选项B的音标是[red]；选项C的音标是[ˈjeloʊ] ，选项B和选项C中的字母e的发音都是[e] ，而选项A中的字母e不发音，因此本题发音不同的选项是选项A，故本题答案为A。

appropriated completely .

两种图形软件种被认为是学习与电力电子系统控制。第一个是在描述更多拨款电力系统，因为它是身体组成。第二个是多拨在描述数字控制信号流

歌曲名:Scar歌手:All Saints专辑:Studio 1Missy Higgins - Scar蓝色血影制作我们暧昧调情,为什么不可以相爱He left a card, a bar of soapAnd a scrubbing brush next to a noteThat said "use these down to your bones"And before I knew I had shiny skinAnd it felt easy being clean like himI thought "this one knows better than I do"A triangle trying to squeeze through a circleHe tried to cut me so I"d fitAnd doesn"t that sound familiar?Doesn"t that hit too close to home?Doesn"t that make you shiver;the way things could"ve gone?And doesn"t it feel peculiarWhen everyone wants a little more?So that I do rememberTo never go that far,Could you leave me with a scar?So the next one came with a bag of treats,She smelled like sugar and spoke like the seaShe told me don"t trust them, trust meThen she pulled at my stitches one by one,Looked at my insides clicking her tongueAnd said "This will all have to come undone"A triangle trying to squeeze through a circleShe tried to blunt me so I"d fitAnd doesn"t that sound familiar?Doesn"t that hit too close to home?Doesn"t that make you shiver;the way things could"ve gone?And doesn"t it feel peculiarWhen everyone wants a little more?So that I do rememberTo never go that far,Could you leave me with a scar?I think I realized just in time,Although my old self was hard to findYou can bathe me in your finest wine but I"ll never give you mine"Cos I"m a little bit tired of fearing that I"ll be the bad fruit nobodyTell me, did you think we"d all dream the same?And doesn"t that sound familiar?Doesn"t that hit too close to home?Doesn"t that make you shiver;the way things could"ve gone?And doesn"t it feel peculiarWhen everyone wants a little more?So that I do rememberTo never go that far,Could you leave me with a scar?Could you leave me with a scar?Could you leave me with a scar?http://music.baidu.com/song/3480228

In the end:在最后at last:终于(主要是时间）Finally：终于e.g:In the end,Mary won.He did it at last.Finally he did it.而且at last只能指时间位置，而不能指时间顺序，在语义上指经过，周折，等待，耽搁后的“最后，终于”得到所期待的结果finally 指一系列事物或论点的顺序，在列举事物时可以用来引出最后一项内容 ，既可指时间位置（相当于at last和in the end），也可指时间的先后顺序。 讲得不太明白~~~~~~~~汗~~~~~~~~~~~~~`可理解为finally最终 ， at last最后， in the end 结果

给DELL打电话800-858-0888 都是上门的..

选C sometime意为“某个时候”,可用于将来或过去来. sometimes 意为“有时”. some time 有两个用法：用作副词词组,意为“任何时候”或“某个时候”(= sometime)；用作名词词组,意为“一段时间”(不能写成 sometime). some times 几次 和 several times 在是一个意思的

歌曲名:Fullmoon歌手:Heavenly专辑:及时行乐Fullmoon -- HeavenlyAll alone late at nightYou never know who you meet in darknessHidden in a secret placeBeing watched from every sidesBe my guide before succombed to the desireHow to look away the fallen angelsCrawling here belowI still resist once and againFind the way out It"s not easyFind the way outThat"s what I am trying to doSlowly gain groundI"m strugging along one more and moreOne more and ~~Find the way outLife is a game (Slowly gain ground )Jus give me a reason to goIf I taste it, should I loveWhen it"s full moon at midnightTurning off the lightThey are screaming wildGive yourself over to absolute pleasureTo attain the highest topSo carry on holding my nameTonight you belong to meSo climbing higher all togetherAnd sailing the seven seasThere"s nothing better here on earthBeware addiction is nearFalling down or gloriousFaced with desire shall we kneelComing closer to myselfI see the devil in your eyesThe dark still remains (remains)A last temptation out of my mindRising up from the ashes of my own graveToo many ideas for you and IRelax or restrainTry to resist once and againFind the way out It"s not easy Slowly gain groundJus give me a reason to goIf I taste it, should I loveWhen it"s full moon at midnightTurning off the lightThey are screaming wildGive yourself over to absolute pleasureTo attain the highest topSo carry on holding my nameTonight you belong to meClimbing higher all togetherAnd sailing the seven seasNothing better here on earthBeware addiction is nearFalling down or gloriousFaced with desire shall we kneel（Solo: Lapauze / Corbiaux ）Find the way out( It"s not easy )Jus give me a reason ~(Slowly gain ground )Jus give me a reason to goIf I taste it, should I loveWhen it"s full moon at midnightTurning off the lightThey are screaming wildGive yourself over to absolute pleasureTo attain the highest topSo carry on holding my nameTonight you belong to meSo climbing higher all togetherAnd sailing the seven seasNothing better here on earthBeware addiction is nearFalling down or gloriousFaced with desire shall we kneelhttp://music.baidu.com/song/19113964

-How much do I owe you for lunch-（ ），It.s nothing.这里应该用Forget it.属于习惯用语。我欠你午餐多少钱呢？算了，没关系。

1、Cell-BasedELISA 的优点： Cell-Based ELISA（基于细胞的 ELISA）是一种全新的ELISA 技术，有两个最突出的优点： 1.1 不需要抽提蛋白、包被微孔板：细胞直接在微孔板里培养，待检测的时候，将细胞固定在微孔板上并 进行通透处理即可。这样就避免了抽提蛋白时，由于客观和主观上引起样品的损失而导致实验结果在一定 程度上偏离了实际情况。同时不用包被微孔板，简化了实验流程，有助于提高效率。科研人员在一个 96 酶联板上，便能检测目标细胞蛋白经刺激或抑制作用后的表现。由于省去抽提蛋白和裂解细胞的步骤，样本的损失也能降到最低，比起其他普通的 ELISA 测定方法，这项全新的 ELISA 技术能更快速、更方便 地一次检测大量的细胞内蛋白。 1.2 可同时检测两种不同蛋白：封闭后加入两种抗不同蛋白且来源于不同宿主的一抗，然后再加入不同的 二抗，加入两种荧光底物，检测两个波长。同时检测两种蛋白的好处是显而易见的，可以减少工作量，此 外还可以满足一些特殊的实验需要，例如，需要测定某个蛋白的磷酸化比例，就需要测定磷酸化蛋白的数 量和总蛋白的数量，这两个测定在同一次实验进行，有助于消除实验误差，得到更为精确的实验结果。 2、Cell-Based ELISA 的两种技术： 某些公司发展了双通道Cell-Based ELISA 技术； 双通道与单通道Cell-based ELISA 比较： 双通道cell-based ELISA，顾名思义，即一次可以同时检测两种目的蛋白，R&D Systems 提供的 Cell-based ELISA 就是双通道cell-based ELISA，原理略：（需要荧光检测方式和相应仪器）； 单通道cell-based ELISA，即一次只能检测一种目的蛋白，他和普通ELISA 的主要区别在于样品处理过 3、cell-basedELISA 的应用： 该产品，最多发展起来的是用于检测磷酸化和非磷酸化蛋白的相对含量； 4、有cell-based ELISA 产品的公司：目前，多家elisa 产品提供 提供cellbased elisa 试剂盒，如Rnd systems， raybiotech， ebioscience， millipore 等公司； 5、如何自己进行 cell based elisa 实验？ 事实上，根据单通道的cell based elisa 原理，可以自己建立cell based elisa 实验系统； 细胞加入培养板中； 加入刺激物或者抑制剂进行培养； 对细胞进行固定或者封闭； 加入第一抗体； 加入HRP 结合的二抗（二抗的选择，同western blot）； 加入底物进行显色；

MilliQ水是millipore 公司的主要产品,其生成的水属于超纯水,即其电阻率为18.2MΩ-cm,但MilliQ又分四种系列( Milli-Q Academic、Milli-Q Biocel、Milli-Q Gradient、Milli-Q Synthesis）,在TOC、热原等方面还是有差异...

【上海巴玖】提示您：一般的离心机达不到说明书上4000g的离心力。

一、Milli-Q water ：Milli-Q水（美国Milli-pore公司Mill-Q超纯水制备系统所生产的水）。例句Purification of Milli-Q water by sub-boiling distilled and employment of qualified FBS could increase the AKP staining positive level and total clone formation rate of mouse ES cells. Q 水经亚沸处理能显著提高ES细胞保持未分化状态的能力，优质胎牛血清能提高ES的AKP阳性度和克隆形成率。二、MilliQ水是millipore 公司的主要产品，其生成的水属于超纯水，即其电阻率为18.2MΩ-cm，但MilliQ又分四种系列( Milli-Q Academic、Milli-Q Biocel、Milli-Q Gradient、Milli-Q Synthesis），在TOC、热原等方面还是有差异的，适用于不同的实验。

angels calling me 天使在呼唤我angels calling me in the night 天使在这个夜里呼唤我something I must see 我必须看的东西something I must see in this night 在这个夜里必须看到的东西such a night 这样的夜晚stars in the sky 星星挂在天上universe is passing by freely I fly with angels...calling me angels calling me 宇宙万物轻轻掠过，我与天使自由地飞翔。天使在呼唤我，在呼唤我angels calling me 天使在呼唤我angels calling me ...in the night 天使在这个夜里呼唤我I am spiraling softly turning free...in the night 在这个夜里，我轻轻地起舞angels whispering as they sing to me on this night 在这个夜里，天使轻轻地在我耳边唱歌such a night 这样的夜晚Stars in the sky 星星挂在天上sister moon is passing by 月亮姐姐路过Freely I fly with the angels...calling me 我和天使自由地飞翔…呼唤我angels calling me天使在呼唤我angels calling me天使在呼唤我angels calling me...in the night天使在这个夜里呼唤我such a night这样的夜晚angels calling 天使在呼唤angels calling 天使在呼唤...... on this night I see... every part of me 在这个夜里，我看到了自己的每一个部分hold this light抓住光It‘s your heart I see 我看到了你的心you are part of me...you are light 你是我的一部分。。。你是光gentle light 温柔的光angels calling ...you are light 天使在呼唤。。。你是光angels calling..hold this light 天使在呼唤。。。抓住光angels calling...you are light 天使在呼唤。。。你是光angels calling ...from the light 天使在呼唤。。。来自光angels calling 天使在呼唤angels calling天使在呼唤angels calling天使在呼唤

原唱/The Surface专辑/周华健《Songs of Birds》编辑/酷狗You know I won"t forget the timeswe shared togetherholding hands and walking in the parkSometime we"ll have to do it all againWe were so happy thenI have no regretsCan"t you tell from the look on my facethat I love you more todayThe first time I looked into your eyes I cried.Do you remember the first time we fell in love?You looked into my eyeswiped the tears awayThe first time when we fell in loveAlthough some time has passedI still remember just like it was yesterday.But time is movin" fastThe love I have for youtime won"t ever changeI"ll always feel the samenow until the endMemories we share will live foreverDeep inside my heartI know I"ll never forgetThe first time I looked into your eyes I cried.Do you remember the first time we fell in love?You looked into my eyeswiped the tears awayThe first time when we fell in loveThe first time I looked into your eyes I cried.Do you remember the first time we fell in love?You looked into my eyeswiped the tears awayThe first time when we fell in loveThe first timehttp://music.baidu.com/song/2642006

We"ve got CD sets and videos, radio and TV shows我们有CD唱片集和影片，广播和电视节目Conferences, retreats and seminars会议，务虚会和研讨会We"ve got books and magazines to read on everything from A to Z我们有书籍和杂志阅读，一切的阅读都是从A到ZAnd a web to surf from anywhere we are通过Web冲浪我们随心所欲But I hope with all this information buzzing through our brains不过，我希望与所有这些信息通过我们的大脑That we will not let our hearts forget the most important thing我们不会忘了最重要的事情Is love, love, love, love, love是爱，爱，爱，爱，爱 It"s all about love, love, love, love, love这都是关于爱，爱，爱，爱，爱 Everything else comes down to this一切都归结于爱Nothing any higher on the list than love没有事情比爱更重要It"s all about love这都是关于爱Now they"re fighting in the middle east现在他们战斗在中东 And they"re fighting down on Seventh Street他们战斗在第七街 And there are fights in my own house on given days在上天赐予的日子里，战斗出现在我们的家园 It"s like something"s lurking deep inside这就像一些事情潜伏在内心深处That can"t seem to be satisfied这似乎无法得到满足 But life was not meant to be lived this way但是生活并不意味着以这种方式延续 "Cause it"s true for every man and woman对于每个男人和女人来说这是一定的 Every boy and girl每一个男孩和女孩 That our only hope for living here together in this world我们的唯一希望是一起生活在这个世界上Is love, love, love, love, love是爱，爱，爱，爱，爱 It"s all about love, love, love, love, love这都是关于爱，爱，爱，爱，爱 Everything else comes down to this一切都归结为这 Nothing any higher on the list than love没有事情比爱更重要的This is the reason we were made这就是我们所作的原因 To know the love of our Creator要明白爱造物主的爱And to give the love He"s given us away并给爱他给我们离开Yeah, the Maker, and the Father, and the God of everything是啊，生产，和父亲，和一切的上帝He says to love, love, love他说，爱，爱，爱He says love, love, love他说，爱，爱，爱Love, love, love爱，爱，爱"Cause after all, it"s all about love终究是关于爱Gods says love, love, love, love, love上帝说，爱，爱，爱，爱，爱It"s all about love, love, love, love, love这都是关于爱，爱，爱，爱，爱Everything else comes down to this一切都归结为爱Nothing any higher on the list than love没有比爱更重要的"Cause after all, it"s all about love终究是关于爱That"s right, yeah (laugh)没错，是的（笑） Woo!It"s all about, yea yea这都是关于爱，是啊是啊It"s all about这都是关于爱All about love, love, love, love, love所有都关于爱，爱，爱，爱，爱It"s all about love, love, love这都是关于爱，爱，爱After all don"t you know?难道你不知道吗？ That it really all comes down to love它确实都归结为爱After all don"t you know that难道你不知道吗？Gonna tell you what it"s all about, yeah要告诉你这一切，是啊Yeah, it"s all about love是的，这都是关于爱Yeah, it"s all about love, yeah是的，这都是关于爱，是的 It"s all about love, 这都是关于爱 Love, love, love爱，爱，爱

4~30度阴凉避光干燥处保管都可以，不可冷冻

rapidly, pushing the tw

超纯水

1、装量：0.5ML 4ML 15ML2、截留分子量3K 10K 30K 50K 100K

Milli-Q纯净水(Milli-Q water),

问：最近要做一些培养基，有一些糖类需要过滤除菌，但不知道怎么操作，还需要什么辅助设备？你只要有滤膜和配套的过滤器就可以了，一般在超净工作台上进行。滤膜用0.22微米或0.45微米两种规格，指的是滤膜的孔径大小吧。一般0.22就够了！如果溶液的量比较大，超过500ml，可以采用已灭菌的抽滤瓶，预先垫好0.22um 的滤膜。抽滤瓶灭菌前要检查封口用纱布或棉花堵牢，使用时，不用在无菌室中进行，只要保证收集瓶无菌状态就可以。抽滤后，过滤好的溶液在无菌条件下倒进已灭菌的空试剂瓶中。如果用小量的溶液过滤，无菌室内用一次性器和一次性0.22um滤膜（millipore或pall都有卖）就可以。 看过滤的量，如果量小，用器加滤器、0.22微米滤膜过滤，滤器加上滤膜后盖紧，稍旋松，饭盒密封100度蒸馏水煮沸30分钟灭菌，用来承接无菌糖水的瓶需高压。用时在无菌操作台上操作，并先旋紧滤器，注意滤器外流下去的哪怕一滴也会造成染菌；过滤应该是费力的，如果感觉很轻松，则滤膜已破。0.45微米的滤膜一般用于两次过滤的第一次，以防止0.22堵塞。器，若能高压更好些。如果量大，正压可用蠕动泵接皮管接不锈钢滤器大张滤膜，这些都是要高压的。负压可用抽滤。

既不是去离子水，也不是蒸馏水。应该是反渗纯水。通过膜处理的反渗水。应该比去离子水、蒸馏水更加纯净，水质决定于反渗膜的质量。millipore rios16对纯净度要求更高。反渗水克服了蒸馏水和去离子水的许多缺点，利用反渗透技术可以有效的去除水中的溶解盐、胶体，细菌、病毒、细菌内毒素和大部分有机物等杂质，电阻率要求更高。

【上海巴玖】提示您：一般的离心机达不到说明书上4000g的离心力。

millipore pure water filter

Millipore water是用 Millipore 公司的超纯水系统制备的去离子水

MilliQ水属于超纯水，DIwater是去离子水，不是一种水。MilliQ水是millipore公司的主要产品，其生成的水属于超纯水。是一种清除了钠，钙，铁，铜，氯化物和溴化物等矿物离子（盐）的纯净水形式。实验室各种水有什么区别。蒸馏水、去离子水、高纯水。

汗……这不是国家名称

1st Ladi - Never Be Replaced 试听下载：http://mp3.baidu.com/m?tn=baidump3&ct=134217728&lm=-1&word=never%20be%20replaced%201st%20LadiBaby I love you and i"ll never let you go 宝贝我爱你 我永远都不会让你离开我But if I have to boy I think that you should know 但是如果有一天不得不离开 我希望你能知道All the love we make can never be erase 我们的爱永远都不会被抹去And i promise you that you will never be replaced 我向你保证 永远都没有人可以取代你Baby I love you and I"ll never let you go 宝贝我爱你 我永远都不会让你离开我But if I have to boy I think that you should know 但是如果有一天不得不离开 我希望你能知道All the love we make can never be erase 我们的爱永远都不会被抹去And i promise you that you will never be replaced 我向你保证 永远没有人能取代你I love you yes i do 我爱你 是的 我真的爱你I"ll be with you as long as you want me too 不管多长时间 我都会永远陪在你身边 只要你希望 Until the end of time 直到时间的尽头From the day I met you I know we"ve be together 从我见到你的那天起我知道我们注定要在一起And now I know I wanna be with you forever 现在 我知道 我想要和你永远在一起I wanna marry you and i wanna have your kids我想和你结婚 我想有我们的孩子 Thinking never compare to feel enough to kisses 想象的感觉永远都无法和亲吻的感觉相比I can say i"m truly happy to the same我可以说 我一样开心 You"ve made me think I"ll die and live my life hesitate你使我意识到我总有一天会死去 我的生活踌躇不前 There"s never been no doubt in my mind 我从来没有过这么坚定不移的觉得That i"ll regret ever having you by my side如果没有你在我身边 我会多么遗憾 But if the day come that i"ll have to let you go但是 如果真的到了那一天 我只能放你走 I think that something I should probably let you know 我想有些事情 我应该让你知道With everything that i spent with you 那些和你一起度过的每一件事Then i will miss you cuz i"m happy that i have you at all我会想念你 因为我很开心 我毕竟曾经拥有过你 I feel for you yes i do我感觉着你的感觉 I"ll be with you as long as you want me to不管多长时间 我都会陪在你身边 只要你希望 Until the end of time 直到时间的尽头

“you say goodbye i say hello hello hello hello ……” 是个女声唱的 这首歌是 Jump5的 Hello Goodbye

coalition 联合执政的多党,团体联合会alliance 两个或多个国家的联盟federation 两个或多个州组成的联邦国家如果提到英联邦的话，特指用commonwealth

一个穿着讲究的人走进了。。。

直译：一个人想找到一个叫Chirton的村庄，他不能找到任何指示标记。于是他决定开车前往一个农舍问一个农民这个村庄的方向。很快他到达了农场的入口。大门上标示着：请随手关门。此人知道这意味着地里有牛，所以他非常小心的把身后的门关上。在他到达下一个门之前，同样的看到了一个标示：请随手关门。于是再一次他走出他的汽车，打开大门，开进去，停下车，下车，关上大门，回到车上继续向前开。很快他就到达了第三个大门。同样的，有请随手关门的标示。再一次这个人离开汽车，打开大门，开车过去，停下车，下车，关上大门，回到车上然后继续开车。在他到达农舍之前他一共做了五次同样的事情。非常幸运的，农夫在门口站着。“我能帮到你什么吗？”农夫问他。“我在找Chirton，请问你知道在哪吗？”"抱歉"，农夫说：“我刚搬过来几个星期而已，我不知道那个地方”。这个人感谢了农夫，然后开车回到路上。返回的时候不得不又开、关了五次农场的大门。当他到达大路上的时候，他回头看这个农场。他看到农夫向他挥手，“嗨！”他想，他一定是想起来Chirton在哪了。他马上返回，停下车，出去，开大门，开车进去，停车，下车，关大门，回到车上然后开车前行。在他到达农场时他不得不又做了四次这样的事情。“你记起来Chirton在哪了吗？”他问农夫。“没有。”农夫回答，“但是我问了我的妻子，她也不知道在哪。”

这种情况一般是刚提到这个人如： A man called Smith wants to see you. 一个叫斯密斯的人想要见你。请及时采纳，不懂继续问( 天天在线 ）(*^__^*) 祝学习进步! 谢谢！

您好，Copyright infringement.版权所有词典[法] all right reserved双语例句版权所有，翻印必究。All rights reserved; those responsible for unauthorized reproduction will be prosecuted.

themanwhoismakingallthatnoiseisrepairingtheroad.whoismakingallthatnoise为定语从句修饰主语theman

MontgomeryTullyMontgomeryTully是一名导演，编剧，代表作品有《外星人体实验》等。外文名：MontgomeryTully职业：导演，编剧代表作品：《外星人体实验》合作人物：MontgomeryTully主要作品人物关系

KateMiller-HeidkeKateMiller-Heidke，11月16日出生于布里斯班，澳大利亚歌手。她的音乐风格俏皮多变，造型也艳丽百变。作品有《TheLastDayOnEarth》等。中文名：凯特米勒外文名：KateMiller-Heidke国籍：澳大利亚星座：天蝎座出生地：布里斯班出生日期：11-16职业：歌手经纪公司：SonyAustralia代表作品：TheLastDayOnEarth主要成就：国际作曲比赛BestFemale；赫尔普曼奖演艺经历2014年，发布音乐专辑《OVertigo！》。音乐作品OVERTIGO!OVertigo！最新唱片：2014年3月14日曲目列表：1.OfferItUp2.YoursWastheBody3.OVertigo!4.ShareYourAir(feat.Passenger)5.RockThisBabyToSleep6.Jimmy7.WhatWasIToYou?8.SingToMe9.Drama(feat.Drapht)10.LoseMyShit11.Ghost(feat.MeganWashington)12.BlissNIGHTFLIGHT发行时间：2012年4月13日曲目列表：1.RideThisFeeling2.Sarah3.Nightflight4.TheTigerInsideWillEattheChild5.LetMeFade6.I"llChangeYourMind7.Humiliation8.IntheDark9.BeautifulDarling10.TheDevilWearsaSuit11.FireandIronFATTYGETSASTYLISTFattyGetsAStylist发行时间：2011年6月24日曲目列表：1.AreYouReady?2.TheDevil"sIntheDetails3.LibertyBell4.TheTigerInsideWillEattheChild5.HollowayPark6.LetMeFade7.YouGottheWorld8.BetterInSpain9.FattyGetsaStylist10.TheFlasher11.ThePlaneWentDownLIVEATTHEHIFI发行时间：2009年10月9日曲目列表：1.OutandIn2.LittleAdam3.ILikeYouBetterWhenYou"reNotAround4.Mama5.PoliticsInSpace6.SpaceTheyCannotTouch7.Dreams/ILoveYou8.AreYouF*ckingKiddingMe9.Motorscooter10.God"sGifttoWomen11.NoTruck12.Words13.You"retheVoice14.CaughtIntheCrowd15.Can"tShakeIt16.WalkingOnaDreamCURIOUSER发行时间：2008年10月18日曲目列表：1.TheOneThingIKnow2.God"sGifttoWomen3.CaughtIntheCrowd4.Can"tShakeIt5.TheLastDayOnEarth6.ILikeYouBetterWhenYou"reNotAround7.Motorscooter8.TheEndofSchool9.PoliticsInSpace10.Supergirl11.OurSong12.NoTruckLITTLEEVE发行时间：2007年6月15日曲目列表：1.IGottheWay2.MakeItLast3.Words4.Delay5.Mama6.SpaceTheyCannotTouch7.Shoebox8.Don"tLetGo9.LittleAdam10.DucksDon"tNeedSatellites11.ThanksforToday12.BoredWithMeCIRCULARBREATHING发行时间：2006年4月22日曲目列表：1.OutandIn2.Jamie3.Apartment4.CavemanDays5.FourSpareSeats6.RiverofDreamsTELEGRAM发行时间：2004年7月14日曲目列表：1.SpaceTheyCannotTouch2.Monster3.Hello4.MyPhotoIntheNewspaper5.BlahBlah6.TheTruth7.TheDayAfterChristmas

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with sth.作状语

①：be filled with侧重于动作和装的东西 The basketball is filled with apples by the old man. ②：be full of 侧重于状态 The bottle is full of water. 区别并不大，主要看你要写句字时的侧重点full是形容词；fill是动词。 full，fill二者均与“满”有关。 full是形容词，意思是“满的”，与of组成be full of短语。 fill做动词，意思是“装满”，与with组成be filled with,表示“用……把……装满”。另外没有 full of 和fill with